Abstract
A simple, efficient, and scalable manufacturing technique is required for developing siRNA-lipid nanoparticles (siRNA-LNP) for therapeutic applications. In this chapter we describe a novel microfluidic-based manufacturing process for the rapid manufacture of siRNA-LNP, together with protocols for characterizing the size, polydispersity, RNA encapsulation efficiency, RNA concentration, and total lipid concentration of the resultant nanoparticles.
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Acknowledgements
This work was supported by the National Science and Engineering Research Council of Canada (F09-04486), Canadian Institutes for Health Research (111627), and Genome British Columbia.
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Walsh, C. et al. (2014). Microfluidic-Based Manufacture of siRNA-Lipid Nanoparticles for Therapeutic Applications. In: Jain, K. (eds) Drug Delivery System. Methods in Molecular Biology, vol 1141. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0363-4_6
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DOI: https://doi.org/10.1007/978-1-4939-0363-4_6
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