Abstract
Over the past 20 years, classic genetic approaches have shown that the developmental program underlying flower formation involves a large number of transcriptional regulators. However, the target genes of these transcription factors, as well as the gene regulatory networks they control, remain largely unknown. Chromatin immunoprecipitation coupled to next-generation sequencing (ChIP-Seq), which allows the identification of transcription factor binding sites on a genome-wide scale, has been successfully applied to a number of transcription factors in Arabidopsis. The ChIP-Seq procedure involves chemical cross-linking of proteins to DNA, followed by chromatin fragmentation and immunoprecipitation of specific protein–DNA complexes. The regions of the genome bound by a specific transcription factor can then be identified after next-generation sequencing.
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Acknowledgements
Work in our laboratory is funded by grants from Science Foundation Ireland to F.W. and E.G.
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Graciet, E., Ó’Maoiléidigh, D.S., Wellmer, F. (2014). Next-Generation Sequencing Applied to Flower Development: ChIP-Seq. In: Riechmann, J., Wellmer, F. (eds) Flower Development. Methods in Molecular Biology, vol 1110. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-9408-9_24
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DOI: https://doi.org/10.1007/978-1-4614-9408-9_24
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