Skip to main content
SpringerLink
Log in

Mass Spectrometric Monitoring of Plant Hormone Cross Talk During Biotic Stress Responses in Potato (Solanum tuberosum L.)

  • Protocol
  • First Online:
Solanum tuberosum

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2354))

Abstract

The potato is among the most important food crops in the world and of incalculable value for global food security. In 2012, the crop area for potato in Northern and Western Europe reached almost 1 million ha and a production of over 37 million tons with an average yield between 18 and 45 tons/ha. However, current potato production is put in jeopardy by a number of important biotic stress factors including late blight (Phytophthora infestans), which was responsible for the disastrous Irish potato famine during 1843–1845. P. infestans shows a remarkable capacity for adaptation with respect to host genotype and applied fungicides. This has made disease management to become more and more difficult and put substantial emphasis on gaining more detailed insight into the molecular bases of plant pathogen interactions, in order to find more sophisticated ways for biological pest control. The plant hormones jasmonic acid (JA) and salicylic acid (SA) play central roles in the regulation of plant responses to biotic foes. In addition, other phytohormones including auxins and abscisic acid (ABA) have also been associated with plant defense responses. For this reason, the parallel analysis of multiple plant hormones in small tissue amounts represents an important field of research in contemporary plant sciences. Here, we describe a highly sensitive and accurate method for the quantitative analysis of ABA, JA, SA, and indole-3-acetic acid in potato plants by gas chromatography-coupled tandem mass spectrometry (GC-MS/MS).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Council NR (1989) Potatoes. In: Lost crops of the Incas: little-known plants of the Andes with promise for worldwide cultivation. The National Academies Press, Washington, DC, pp 92–103

    Google Scholar 

  2. Spooner DM, McLean K, Ramsay G et al (2005) A single domestication for potato based on multilocus amplified fragment length polymorphism genotyping. Proc Natl Acad Sci U S A 102:14694–14699

    Article  CAS  Google Scholar 

  3. Andre CM, Legay S, Iammarino C et al (2014) The potato in the human diet: a complex matrix with potential health benefits. Potato Res 57:201–214

    Article  CAS  Google Scholar 

  4. Davies PJ (2010) Plant hormones. Biosynthesis, signal transduction, action! 3rd edn. Springer, Dordrecht

    Google Scholar 

  5. Dong X (1998) SA, JA, ethylene, and disease resistance in plants. Curr Opin Plant Biol 1:316–323

    Article  CAS  Google Scholar 

  6. Kazan K, Manners JM (2009) Linking development to defense: auxin in plant-pathogen interactions. Trends Plant Sci 14:373–382

    Article  CAS  Google Scholar 

  7. Robert-Seilaniantz A, Grant M, Jones JDG (2011) Hormone crosstalk in plant disease and defense: more than just Jasmonate-Salicylate antagonism. Annu Rev Phytopathol 49:317–343

    Article  CAS  Google Scholar 

  8. Denancé N, Sánchez-Vallet A, Goffner D et al (2013) Disease resistance or growth: the role of plant hormones in balancing immune responses and fitness costs. Front Plant Sci 4:155

    Article  Google Scholar 

  9. Kazan K, Manners JM (2008) Jasmonate signaling: toward an integrated view. Plant Physiol 146:1459–1468

    Article  CAS  Google Scholar 

  10. Wolters H, Jürgens G (2009) Survival of the flexible: hormonal growth control and adaptation in plant development. Nat Rev Genet 10:305–317

    Article  CAS  Google Scholar 

  11. Hoffmann M, Hentrich M, Pollmann S (2011) Auxin-oxylipin crosstalk: relationship of antagonists. J Integr Plant Biol 53:429–445

    Article  CAS  Google Scholar 

  12. Tiryaki I, Staswick PE (2002) An Arabidopsis mutant defective in jasmonate response is allelic to the auxin-signaling mutant axr1. Plant Physiol 130:887–894

    Article  CAS  Google Scholar 

  13. Ren C, Pan J, Peng W et al (2005) Point mutations in Arabidopsis Cullin1 reveal its essential role in jasmonate response. Plant J 42:514–524

    Article  CAS  Google Scholar 

  14. Szemenyei H, Hannon M, Long JA (2008) TOPLESS mediates auxin-dependent transcriptional repression during Arabidopsis embryogenesis. Science 319:1384–1386

    Article  CAS  Google Scholar 

  15. Dombrecht B, Xue GP, Sprague SJ et al (2007) MYC2 differentially modulates diverse jasmonate-dependent functions in Arabidopsis. Plant Cell 19:2225–2245

    Article  CAS  Google Scholar 

  16. Hentrich M, Böttcher C, Düchting P et al (2013) The jasmonic acid signaling pathway is linked to auxin homeostasis through the modulation of YUCCA8 and YUCCA9 gene expression. Plant J 74:626–637

    Article  CAS  Google Scholar 

  17. Sun J, Chen Q, Qi L et al (2011) Jasmonate modulates endocytosis and plasma membrane accumulation of the Arabidopsis PIN2 protein. New Phytol 191:360–375

    Article  CAS  Google Scholar 

  18. Sun J, Xu Y, Ye S et al (2009) Arabidopsis ASA1 is important for jasmonate-mediated regulation of auxin biosynthesis and transport during lateral root formation. Plant Cell 21:1495–1511

    Article  CAS  Google Scholar 

  19. Navarro L, Dunoyer P, Jay F et al (2006) A plant miRNA contributes to antibacterial resistance by repressing auxin signaling. Science 312:436–439

    Article  CAS  Google Scholar 

  20. Müller A, Düchting P, Weiler EW (2002) A multiplex GC-MS/MS technique for the sensitive and quantitative single-run analysis of acidic phytohormones and related compounds, and its application to Arabidopsis thaliana. Planta 216:44–56

    Article  Google Scholar 

  21. Schmelz EA, Engelberth J, Alborn HT et al (2003) Simultaneous analysis of phytohormones, phytotoxins, and volatile organic compounds in plants. Proc Natl Acad Sci U S A 100:10552–10557

    Article  CAS  Google Scholar 

  22. Birkemeyer C, Kolasa A, Kopka J (2003) Comprehensive chemical derivatization for gas chromatography-mass spectrometry-based multi-targeted profiling of the major phytohormones. J Chromatogr A 993:89–102

    Article  CAS  Google Scholar 

  23. Chiwocha SD, Abrams SR, Ambrose SJ et al (2003) A method for profiling classes of plant hormones and their metabolites using liquid chromatography-electrospray ionization tandem mass spectrometry: an analysis of hormone regulation of thermodormancy of lettuce (Lactuca sativa L.) seeds. Plant J 35:405–417

    Article  CAS  Google Scholar 

  24. Ziegler J, Qwegwer J, Schubert M et al (2014) Simultaneous analysis of apolar phytohormones and 1-aminocyclopropane-1-carboxylic acid by high performance liquid chromatography/electrospray negative ion tandem mass spectrometry via 9-fluorenylmethoxycarbonyl chloride derivatization. J Chromatogr A 1362:102–109

    Article  CAS  Google Scholar 

  25. Strehmel N, Böttcher C, Schmidt S et al (2014) Profiling of secondary metabolites in root exudates of Arabidopsis thaliana. Phytochemistry 108:35–46

    Article  CAS  Google Scholar 

  26. Wang X, Zhao P, Liu X et al (2014) Quantitative profiling method for phytohormones and betaines in algae by liquid chromatography electrospray ionization tandem mass spectrometry. Biomed Chromatogr 28:275–280

    Article  Google Scholar 

  27. Novák O, Hényková E, Sairanen I et al (2012) Tissue-specific profiling of the Arabidopsis thaliana auxin metabolome. Plant J 72:523–536

    Article  Google Scholar 

  28. Åstot C, Dolezal K, Moritz T et al (1998) Precolumn derivatization and capillary liquid chromatographic/frit-fast atom bombardment mass spectrometric analysis of cytokinins in Arabidopsis thaliana. J Mass Spectrom 33:892–902

    Article  Google Scholar 

  29. Jameson PE, Zhang H, Lewis DH (2000) Cytokinins. Extraction, separation, and analysis. Methods Mol Biol 141:101–121

    CAS  PubMed  Google Scholar 

  30. Farrow S, Emery RN (2012) Concurrent profiling of indole-3-acetic acid, abscisic acid, and cytokinins and structurally related purines by high-performance-liquid-chromatography tandem electrospray mass spectrometry. Plant Methods 8:42

    Article  CAS  Google Scholar 

  31. Kojima M, Sakakibara H (2012) Highly sensitive high-throughput profiling of six phytohormones using MS-probe modification and liquid chromatography–tandem mass spectrometry. In: Normanly J (ed) High-throughput phenotyping in plants, Methods in molecular biology, vol 918. Humana Press, Totowa, NJ, pp 151–164

    Chapter  Google Scholar 

  32. Takei K, Yamaya T, Sakakibara H (2003) A method for separation and determination of cytokinin nucleotides from plant tissues. J Plant Res 116:265–269

    Article  CAS  Google Scholar 

  33. Novák O, Hauserová E, Amakorová P et al (2008) Cytokinin profiling in plant tissues using ultra-performance liquid chromatography–electrospray tandem mass spectrometry. Phytochemistry 69:2214–2224

    Article  Google Scholar 

Download references

Acknowledgments

The authors are thankful for the received financial support from the Ministry of Economy and Competitiveness (MINECO) of Spain, grant BFU2017-82826-R to SP. J.M. was supported by the “Severo Ochoa Program for Centers of Excellence in R&D” from the Agencia Estatal de Investigación of Spain, grant SEV-2016-0672 (2017–2021) to the CBGP.

Author information

Authors and Affiliations

  1. Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)—Instituto Nacional de Investigación y Tecnología Agraria y Alimentación (INIA), Madrid, Spain

    Marta-Marina Pérez-Alonso, Paloma Ortiz-García, José Moya-Cuevas & Stephan Pollmann

  2. Departamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid (UPM), Madrid, Spain

    Stephan Pollmann

Authors
  1. Marta-Marina Pérez-Alonso
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Paloma Ortiz-García
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. José Moya-Cuevas
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Stephan Pollmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Stephan Pollmann .

Editor information

Editors and Affiliations

  1. Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia

    David Dobnik

  2. Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia

    Kristina Gruden

  3. Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia

    Živa Ramšak

  4. Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia

    Anna Coll

Rights and permissions

Reprints and permissions

Copyright information

© 2021 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Pérez-Alonso, MM., Ortiz-García, P., Moya-Cuevas, J., Pollmann, S. (2021). Mass Spectrometric Monitoring of Plant Hormone Cross Talk During Biotic Stress Responses in Potato (Solanum tuberosum L.). In: Dobnik, D., Gruden, K., Ramšak, Ž., Coll, A. (eds) Solanum tuberosum. Methods in Molecular Biology, vol 2354. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1609-3_7

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-1609-3_7

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1608-6

  • Online ISBN: 978-1-0716-1609-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation