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In Vitro Generation of Vascular Wall–Typical Mesenchymal Stem Cells (VW-MSC) from Murine Induced Pluripotent Stem Cells Through VW-MSC–Specific Gene Transfer

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Stem Cells and Tissue Repair

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2155))

Abstract

Among the adult stem cells, multipotent mesenchymal stem cells (MSCs) turned out to be a promising option for cell-based therapies for the treatment of various diseases including autoimmune and cardiovascular disorders. MSCs bear a high proliferation and differentiation capability and exert immunomodulatory functions while being still clinically safe. As tissue-resident stem cells, MSCs can be isolated from various tissue including peripheral or umbilical cord blood, placenta, blood, fetal liver, lung, adipose tissue, and blood vessels, although the most commonly used source for MSCs is the bone marrow. However, the proportion of MSCs in primary isolates from adult tissue biopsies is rather low, and therefore MSCs must be intensively expanded in vitro before the MSCs find particular use in therapies that may require extensive and repetitive cell replacement. Therefore, more easily accessible sources of MSCs are needed. Here, we present a detailed protocol to generate tissue-typical MSCs by direct linage conversion using transcription factors defining target MSC identity from murine induced pluripotent stem cells (iPSCs).

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Acknowledgments

The Jürgen Manchot Stiftung (Düsseldorf, Germany) supported this work.

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Authors and Affiliations

  1. Institute for Cell Biology (Cancer Research), Medical Faculty, University of Duisburg-Essen, Essen, Germany

    Jennifer Steens

  2. Institute for Transfusion Medicine, Medical Faculty, University of Duisburg-Essen, Essen, Germany

    Hannes Klump

  3. Institute for Cell Biology (Cancer Research), Medical Faculty, University of Duisburg-Essen, Essen, Germany

    Diana Klein

Authors
  1. Jennifer Steens
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  2. Hannes Klump
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  3. Diana Klein
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Corresponding author

Correspondence to Diana Klein .

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Editors and Affiliations

  1. Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR, USA

    Chrissa Kioussi

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Steens, J., Klump, H., Klein, D. (2020). In Vitro Generation of Vascular Wall–Typical Mesenchymal Stem Cells (VW-MSC) from Murine Induced Pluripotent Stem Cells Through VW-MSC–Specific Gene Transfer. In: Kioussi, C. (eds) Stem Cells and Tissue Repair . Methods in Molecular Biology, vol 2155. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0655-1_7

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  • DOI: https://doi.org/10.1007/978-1-0716-0655-1_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0654-4

  • Online ISBN: 978-1-0716-0655-1

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