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Characterization of Plant Viruses pp 449–463Cite as

Development of Infectious Clone of Virus

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Abstract

An infectious clone is a full-length DNA clone from which infectious transcripts can be obtained in vitro or in vivo with a suitable promoter. An infectious clone is the basic need to study functional genomics, replication and expression of viral proteins and in understanding host–virus interactions. Infectious clones, considered as the pools of viral genes for designing antiviral strategies, are an essential source of material for the preparation of new viral vectors. In this chapter, production of in vitro and in vivo clones of viruses is discussed.

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Authors and Affiliations

  1. Division of Crop Protection, ICAR-Indian Institute of Spices Research, Kozhikode, Kerala, India

    Alangar Ishwara Bhat

  2. Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, India

    Govind Pratap Rao

Authors
  1. Alangar Ishwara Bhat
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  2. Govind Pratap Rao
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Bhat, A.I., Rao, G.P. (2020). Development of Infectious Clone of Virus. In: Characterization of Plant Viruses . Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0334-5_46

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  • DOI: https://doi.org/10.1007/978-1-0716-0334-5_46

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0333-8

  • Online ISBN: 978-1-0716-0334-5

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