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A Carrier Strategy for Mass Cytometry Analysis of Small Numbers of Cells

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Book cover T-Cell Receptor Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2111))

Abstract

The recent launch of mass cytometry or cytometry by time of flight (CyTOF) has revolutionized flow cytometry. Similar to fluorescence flow cytometry, a key challenge for CyTOF is to analyze samples of limited amount or very rare cell populations under various experimental settings. Here we describe a carrier strategy that significantly reduces the required sample amount without losing analytical resolution. We were able to detect as few as 5 × 104 human peripheral blood mononuclear cells (PBMCs) using this method. This simple method thus enables the maximal usage of valuable clinical samples.

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Acknowledgments

This work was supported by the Natural Science Foundation of Fujian Province of China No.2018 J05065, to LZ, and National Natural Science Foundation of China grants 31770952, 31570911, and 2017ZX10202203-003- 001 to G.F.

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Authors and Affiliations

  1. State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen, China

    Xian Jia, Xiaojuan Zhou, Haiping Zheng, Shan Jiang, Jiannan Weng, Lei Huang, Changchun Xiao, Lei Zhang, Xiao Lei Chen & Guo Fu

  2. Innovation Center, Shanghai Benemae Pharmaceutical Corporation, Shanghai, China

    Zhiqiang Du

  3. Cancer Research Center of Xiamen University, Xiamen, China

    Changchun Xiao & Guo Fu

Authors
  1. Xian Jia
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  2. Xiaojuan Zhou
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  3. Haiping Zheng
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  4. Shan Jiang
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  5. Jiannan Weng
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  6. Lei Huang
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  7. Zhiqiang Du
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  8. Changchun Xiao
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  9. Lei Zhang
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  10. Xiao Lei Chen
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  11. Guo Fu
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Corresponding authors

Correspondence to Lei Zhang , Xiao Lei Chen or Guo Fu .

Editor information

Editors and Affiliations

  1. Department of Pathogen Biology, Huazhong University of Science and Technology, Wuhan, China

    Chaohong Liu

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Jia, X. et al. (2020). A Carrier Strategy for Mass Cytometry Analysis of Small Numbers of Cells. In: Liu, C. (eds) T-Cell Receptor Signaling. Methods in Molecular Biology, vol 2111. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0266-9_2

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  • DOI: https://doi.org/10.1007/978-1-0716-0266-9_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0265-2

  • Online ISBN: 978-1-0716-0266-9

  • eBook Packages: Springer Protocols

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