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Immune Monitoring of Human Mucosal-Associated Invariant T Cells by Quantitative Proteomics

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MAIT Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2098))

Abstract

Molecular phenotypes of mucosal-associated invariant T (MAIT) cells are correlating with individual susceptibilities and outcomes in human diseases. Quantitative proteome strategies can examine such variations in the functional and druggable inventory of MAIT cells comprehensively, but protocols for the support of translational and clinical studies are still rare. Here, we describe a protocol in which MR1-restricted MAIT cells were isolated from blood donations by FACS and are then characterized by quantitative proteomics (iTRAQ-LC-MS/MS) to complement information about their unique effector phenotype and to investigate donor-/patient- or disease-specific variations in protein networks with high precision.

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Author information

Authors and Affiliations

  1. Cellular Proteomics, Helmholtz Centre for Infection Research, Braunschweig, Germany

    Isabel Bernal, Marco van Ham & Lothar Jänsch

  2. Institute of Medical Microbiology and Hospital Hygiene, Infection Immunology, Otto-von-Guericke University Magdeburg, Magdeburg, Germany

    Isabel Bernal

  3. ESF Graduate School ABINEP, Magdeburg, Germany

    Isabel Bernal

Authors
  1. Isabel Bernal
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  2. Marco van Ham
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  3. Lothar Jänsch
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Corresponding author

Correspondence to Lothar Jänsch .

Editor information

Editors and Affiliations

  1. Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden, Clinical Immunology and Transfusion Medicine, Karolinska University Hospital, Stockholm, Sweden

    Helen Kaipe

  2. Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden

    Isabelle Magalhaes

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Bernal, I., van Ham, M., Jänsch, L. (2020). Immune Monitoring of Human Mucosal-Associated Invariant T Cells by Quantitative Proteomics. In: Kaipe, H., Magalhaes, I. (eds) MAIT Cells. Methods in Molecular Biology, vol 2098. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0207-2_14

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  • DOI: https://doi.org/10.1007/978-1-0716-0207-2_14

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0206-5

  • Online ISBN: 978-1-0716-0207-2

  • eBook Packages: Springer Protocols

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