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Imaging and Tracking Stem Cells pp 113–120Cite as

Efficient Labeling of Human Mesenchymal Stem Cells Using Iron Oxide Nanoparticles

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2150))

Abstract

Stem cells have been used in multiple clinical trials. Tracking these transplanted cells in vivo will provide real-time information on the fate of these cells. Iron oxide labeling is one such uncomplicated noninvasive labeling method. These transformed nanocrystals can be used for varied applications including stem-cell tracking, magnetic resonance imaging, and theranostics. Here we elucidate the protocol for iron oxide nanoparticles synthesis (IONPS) and labeling of mesenchymal stem cells which can be used for imaging and tracking cells to understand their fate in in vivo studies.

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Acknowledgments

This work was supported by Department of Biotechnology, Government of India.

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Author notes

  1. Sonali Rawat and Suchi Gupta contributed equally to this work.

Authors and Affiliations

  1. Stem Cell Facility (DBT-Center of Excellence for Stem Cell Research), All India Institute of Medical Sciences, New Delhi, India

    Sonali Rawat, Suchi Gupta & Sujata Mohanty

  2. Department of Pathology, All India Institute of Medical Sciences, New Delhi, India

    Madhusudan Bhat & Amit Kumar Dinda

Authors
  1. Sonali Rawat
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  2. Suchi Gupta
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  3. Madhusudan Bhat
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  4. Amit Kumar Dinda
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  5. Sujata Mohanty
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Editor information

Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Rawat, S., Gupta, S., Bhat, M., Dinda, A.K., Mohanty, S. (2019). Efficient Labeling of Human Mesenchymal Stem Cells Using Iron Oxide Nanoparticles. In: Turksen, K. (eds) Imaging and Tracking Stem Cells. Methods in Molecular Biology, vol 2150. Humana, New York, NY. https://doi.org/10.1007/7651_2019_265

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  • DOI: https://doi.org/10.1007/7651_2019_265

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0626-1

  • Online ISBN: 978-1-0716-0627-8

  • eBook Packages: Springer Protocols

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