Abstract
The study of skin pigmentation requires determining the rate of melanin production in melanocytes and quantifying the rate of melanosome transfer to keratinocytes. Here, we describe a method to quantify melanosome transfer using immunofluorescence microscopy coupled with automated image analysis of in vitro human melanocytes and keratinocytes in co-culture. In this method, the number of melanin capped keratinocyte nuclei is quantified.
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References
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Sridharan, A., John Lim, S.Y., Wright, G.D., Vardy, L.A. (2019). Quantification of Melanosome Transfer Using Immunofluorescence Microscopy and Automated Image Analysis. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology, vol 2109. Humana, New York, NY. https://doi.org/10.1007/7651_2019_241
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DOI: https://doi.org/10.1007/7651_2019_241
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0250-8
Online ISBN: 978-1-0716-0251-5
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