Abstract
Immunohistochemistry (IHC) is a frequently used technique in life science and in clinic diagnostic. IHC is a high precision method to localize different cell types or their expression in tissue. Over the years, different approaches of IHC have emerged, and the technique has become more and more sophisticated. However, the principles still remains: the inherent and spontaneous non-covalent interaction between an antibody and (hypothetical) any target of interest. That means, using this technique allows you to analyze a wide range of histological tissues (muscles, organs, neurons, etc.) from humans or animals under the microscope. Literally, IHC makes the invisible to the human eye clearly visible. In this chapter, we present an approach how to analyze human skeletal muscle tissue for content and activity of muscle stem cells, termed satellite cells.
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Abbreviations
- BSA:
-
Bovine serum albumin
- DAPI:
-
4′,6-Diamidino-2-phenylindole
- FBS:
-
Fetal bovine serum
- GS:
-
Goat serum
- Pax7:
-
Paired box protein-7
- SC:
-
Satellite cell
- PBS:
-
Phosphate buffered saline
- MHC-I:
-
Myosin heavy chain I
- nMHC:
-
Neonatal myosin heavy chain
References
Duraiyan J, Govindarajan R, Kaliyappan K, Palanisamy M (2012) Applications of immunohistochemistry. J Pharm Bioallied Sci 4(Suppl 2):S307–S309. https://doi.org/10.4103/0975-7406.100281
Boutrup RJ, Farup J, Vissing K, Kjaer M, Mikkelsen UR (2018) Skeletal muscle stem cell characteristics and myonuclei content in patients with rheumatoid arthritis: a cross-sectional study. Rheumatol Int 38(6):1031–1041. https://doi.org/10.1007/s00296-018-4028-y
Kalyuzhny AE (2016) Immunohistochemistry. Essential elements and beyond, vol 36(6), Springer International, Cham, p 3226. doi:https://doi.org/10.1007/978-3-319-30893-7
Ramos-Vara JA, Miller MA (2014) When tissue antigens and antibodies get along: revisiting the technical aspects of immunohistochemistry—the red, brown, and blue technique. Vet Pathol 51(1):42–87. https://doi.org/10.1177/0300985813505879
Acknowledgments
I would like to thank Jean Farup for introducing me to the amazing world of immunohistochemistry and for sharing all his great knowledge about muscle and satellite cells. I would also thank my colleague on VIA University College, Malene Munk Jørgensen, for her critical review of this manuscript.
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Boutrup, R.J. (2019). Analysis of Stem Cells and Their Activity in Human Skeletal Muscles by Immunohistochemistry. In: Turksen, K. (eds) Stem Cells and Aging . Methods in Molecular Biology, vol 2045. Humana, New York, NY. https://doi.org/10.1007/7651_2019_215
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DOI: https://doi.org/10.1007/7651_2019_215
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9712-1
Online ISBN: 978-1-4939-9713-8
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