Abstract
An overall three-dimensional picture of the distribution of epidermal cells at a given time point is of importance to better characterize epidermal progenitors. We introduce a whole-mount immunofluorescent staining coupled to a multicolor lineage tracing model for analyzing the spatiotemporal organization of epidermal stem cells. Laser scanning confocal microscopy with multiple labelling allows for robust imaging of dorsal skin with excellent resolution.
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Acknowledgements
This work was supported by the Australian Research Council Discovery Project DP130104777 and the National Health and Medical Research Council project grant 1145350.
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Roy, E., Khosrotehrani, K. (2018). Whole-Mount Immunofluorescent Staining Coupled to Multicolor Lineage Tracing Model for Analyzing the Spatiotemporal Organization of Epidermal Stem Cells. In: Turksen, K. (eds) Skin Stem Cells. Methods in Molecular Biology, vol 1879. Humana Press, New York, NY. https://doi.org/10.1007/7651_2018_165
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DOI: https://doi.org/10.1007/7651_2018_165
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8869-3
Online ISBN: 978-1-4939-8870-9
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