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Differential Adipose Tissue Proteomics

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Tissue Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1788))

Abstract

Differential proteomic analysis (comparative quantitative proteomics) is a robust quantitative technique used to detect and identify the proteome of selected tissues. The expression levels (upregulated vs. downregulated) of proteins in tissue samples that differ by experimental design or anatomic location are determined by a series of assays including (1) 2D difference gel electrophoresis (2D-DiGE), (2) protein spot picking based on a priori thresholds, (3) Mass Spectrometry, and (4) follow-up Western Blot for antibody validation (Chen et al., Mol Cell Proteomics 14:2466–2478, 2015). Differential proteomic analysis is a perfect method for analyzing a heterogeneous tissue such as adipose tissue with a composition spectrum consisting of white to brown adipocytes along with a stromal vascular fraction dependent on anatomical location and inflammation. The adipose tissue proteomic protocol outlined here was successful in identifying differentially expressed proteins both significantly upregulated and downregulated between the experimental and control groups (Shields et al., Pulm Circ 6:586–596, 2016).

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Author information

Authors and Affiliations

  1. Department of Medicine, Lupus Center of Excellence – Autoimmunity Institute, Allegheny Health Network, Pittsburgh, PA, USA

    Kelly J. Shields

  2. Allegheny General Hospital, 320 E North Avenue, 8th FL South Tower, Pittsburgh, PA, 15212, USA

    Kelly J. Shields

  3. Center for Advanced Proteomics Research, New Jersey Medical School Rutgers, The State University of New Jersey, 185 south Orange Ave, Newark, NJ, 07103, USA

    Changgong Wu

Authors
  1. Kelly J. Shields
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  2. Changgong Wu
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Corresponding author

Correspondence to Kelly J. Shields .

Editor information

Editors and Affiliations

  1. Professor, Department of Surgery Medicine and Pediatrics Director, Kidney-Pancreas Transplant Program, University of California San Francisco, San Francisco, California, USA

    Minnie M. Sarwal

  2. Asst. Professor, Department of Surgery, University of California San Francisco, San Francisco, California, USA

    Tara K. Sigdel

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© 2017 Springer Science+Business Media New York

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Shields, K.J., Wu, C. (2017). Differential Adipose Tissue Proteomics. In: Sarwal, M., Sigdel, T. (eds) Tissue Proteomics. Methods in Molecular Biology, vol 1788. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_80

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  • DOI: https://doi.org/10.1007/7651_2017_80

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7852-6

  • Online ISBN: 978-1-4939-7854-0

  • eBook Packages: Springer Protocols

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