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Measuring Redox Status of Melanoma Cells

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Part of the book series: Methods in Molecular Biology

Abstract

Redox homeostasis plays multiple roles in essentially all aspects of cellular function, and hence, reliable methods for measuring cellular or tissue redox status are key elements in understanding the redox related signal pathways. However, in the free radical biology field, there are many controversies on the methods to measure reactive oxygen species. In this chapter we describe our experience in measuring superoxide, hydrogen peroxide, and a general redox status using redox-sensitive green fluorescence proteins (roGFPs) in human melanoma cells.

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Acknowledgements

This study is supported by Oxnard Foundation and Waltmar Foundation to F.L.M., and Department of Medicine Allan Hubbell Education Funds (University of California Irvine) to F.L.S.

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Authors and Affiliations

  1. Department of Epidemiology, Chao Family Comprehensive Cancer Center, University of California Irvine School of Medicine, B200 Sprague Hall, 839 Health Science Road, Irvine, CA, 92697, USA

    Feng Liu-Smith

  2. Beckman Laser Institute and Medical Clinic, University of California Irvine, Irvine, CA, 92697, USA

    Tatiana B. Krasieva

  3. The State Key Laboratory of Medical Genetics and School of Life Sciences, Central South University, Changsha, Hunan Province, China

    Jing Liu

  4. Institute of Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Xi’an Jiaotong University School of Life Science and Technology, Xi’an, Shaangxi Province, China

    Jiankang Liu

  5. Department of Medicine, Chao Family Comprehensive Cancer Center, University of California Irvine School of Medicine, B200 Sprague Hall, 839 Health Science Road, Irvine, CA, 92697, USA

    Frank L. Meyskens Jr.

Authors
  1. Feng Liu-Smith
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  2. Tatiana B. Krasieva
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  3. Jing Liu
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  4. Jiankang Liu
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  5. Frank L. Meyskens Jr.
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Corresponding author

Correspondence to Feng Liu-Smith .

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© 2016 Springer Science+Business Media New York

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Liu-Smith, F., Krasieva, T.B., Liu, J., Liu, J., Meyskens, F.L. (2016). Measuring Redox Status of Melanoma Cells. In: Methods in Molecular Biology. Humana Press. https://doi.org/10.1007/7651_2016_352

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  • DOI: https://doi.org/10.1007/7651_2016_352

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  • Publisher Name: Humana Press

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