Overview
- Provides an easily accessible reference volume for state-of-the-art experimentation by leading researchers in the field
- Covers a wide range of approaches for the isolation and characterization of macrophages and dendritic cells
- Provides detailed step-by-step protocols to modify gene expression in cells that are notoriously resistant to genetic manipulation
- Provides approaches for detailed quantitative functional analyses including activation states and lipid signaling
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 531)
Access this book
Tax calculation will be finalised at checkout
Other ways to access
Table of contents (21 protocols)
Keywords
About this book
In light of the critical contributions of macrophages and dendritic cells to diverse inflammatory diseases and to immunity and host defense, state-of-the-art approaches to the investigation of their behavior are essential. In Macrophages and Dendritic Cells: Methods and Protocols, expert researchers contribute laboratory protocols involving these two vital cell types functioning at the junction of the innate and acquired immune systems. The volume delves first into isolation and cell culturing then continues with topics such as phagocytosis, genetic manipulation, macrophage activation, and lipid signaling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Macrophages and Dendritic Cells: Methods and Protocols provides a timely and useful guide for both seasoned investigators and neophytes pursuing this imperative field of study.
Reviews
Bibliographic Information
Book Title: Macrophages and Dendritic Cells
Book Subtitle: Methods and Protocols
Editors: Neil E. Reiner
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-396-7
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-58829-972-7Published: 01 April 2009
Softcover ISBN: 978-1-62703-849-2Published: 26 November 2014
eBook ISBN: 978-1-59745-396-7Published: 05 April 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 368
Topics: Immunology, Cell Culture