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Lipidomics

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Sanjoy K. Bhattacharya

    1. University of Miami, Miami, USA

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1609)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Lipid Sample Preparation for Biomedical Research

    • Ravin Sajnani, Katyayini Aribindi
    Pages 1-7

  3. Lipid Extraction Techniques for Stable Isotope Analysis and Ecological Assays

    • Kyle H. Elliott, James D. Roth, Kevin Crook
    Pages 9-24

  4. Isolation of Lipid Raft Proteins from CD133+ Cancer Stem Cells

    • Vineet K. Gupta, Sulagna Banerjee
    Pages 25-31

  5. Isolation of Neuronal Synaptic Membranes by Sucrose Gradient Centrifugation

    • Blake R. Hopiavuori, Dustin R. Masser, Joseph L. Wilkerson, Richard S. Brush, Nawajes A. Mandal, Robert E. Anderson et al.
    Pages 33-41

  6. Sample Preparation and Analysis for Imaging Mass Spectrometry

    • Genea Edwards, Annia Mesa, Robert I. Vazquez-Padron, Jane-Marie Kowalski, Sanjoy K. Bhattacharya
    Pages 43-50

  7. Direct Measurement of Free and Esterified Cholesterol Mass in Differentiated Human Podocytes: A TLC and Enzymatic Assay-Based Method

    • Christopher E. Pedigo, Sandra M. Merscher, Alessia Fornoni
    Pages 51-56

  8. High-Performance Chromatographic Separation of Cerebrosides

    • Renaud Sicard, Ralf Landgraf
    Pages 57-63

  9. Lipid Identification by Untargeted Tandem Mass Spectrometry Coupled with Ultra-High-Pressure Liquid Chromatography

    • Gabriel B. Gugiu
    Pages 65-82

  10. Utility of Moderate and High-Resolution Mass Spectrometry for Class-Specific Lipid Identification and Quantification

    • Maria del Carmen Piqueras
    Pages 83-90

  11. A Robust Lipidomics Workflow for Mammalian Cells, Plasma, and Tissue Using Liquid-Chromatography High-Resolution Tandem Mass Spectrometry

    • Candice Z. Ulmer, Rainey E. Patterson, Jeremy P. Koelmel, Timothy J. Garrett, Richard A. Yost
    Pages 91-106

  12. Combined Use of MALDI-TOF Mass Spectrometry and 31P NMR Spectroscopy for Analysis of Phospholipids

    • Jenny Schröter, Yulia Popkova, Rosmarie Süß, Jürgen Schiller
    Pages 107-122

  13. Global Monitoring of the Mammalian Lipidome by Quantitative Shotgun Lipidomics

    • Inger Ødum Nielsen, Kenji Maeda, Mesut Bilgin
    Pages 123-139

  14. Bioinformatics Pertinent to Lipid Analysis in Biological Samples

    • Justin Ma, Ulises Arbelo, Yenifer Guerra, Katyayini Aribindi, Sanjoy K. Bhattacharya, Daniel Pelaez
    Pages 141-147

  15. LC–MS-Based Lipidomics and Automated Identification of Lipids Using the LipidBlast In-Silico MS/MS Library

    • Tomas Cajka, Oliver Fiehn
    Pages 149-170

  16. Single-Step Capture and Targeted Metabolomics of Alkyl-Quinolones in Outer Membrane Vesicles of Pseudomonas aeruginosa

    • Pallavi Lahiri, Dipankar Ghosh
    Pages 171-184

  17. Analysis of Fatty Acid and Cholesterol Content from Detergent-Resistant and Detergent-Free Membrane Microdomains

    • Mark E. McClellan, Michael H. Elliott
    Pages 185-194

  18. Computational Functional Analysis of Lipid Metabolic Enzymes

    • Carolina Bagnato, Arjen Ten Have, María B. Prados, María V. Beligni
    Pages 195-216

  19. Isoprenylation of Monomeric GTPases in Human Trabecular Meshwork Cells

    • Evan B. Stubbs Jr.
    Pages 217-229

  20. Purification and Validation of Lipid Transfer Proteins

    • Matti A. Kjellberg, Anders P. E. Backman, Anna Möuts, Peter Mattjus
    Pages 231-239
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Keywords

About this book

This volume explores lipidomics through protocols that focus on areas of utility, techniques, and bioinformatics advancements. The protocols in this book cover topics such as isolation of specific membranes and specialized fractionation of subcellular compartments, and computational and functional analysis of lipid metabolizing enzymes. Each protocol also discusses methods of dealing with fractionated organelles—cells to whole organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Lipidomics: Methods and Protocols is a useful and valuable resource for biochemists, molecular biologists, and neuroscientists interested in neurology, ophthalmology and vision sciences, as well as massspectrometrists with interest in disease discovery.

 

Editors and Affiliations

  • University of Miami, Miami, USA

    Sanjoy K. Bhattacharya

Bibliographic Information

  • Book Title: Lipidomics

  • Book Subtitle: Methods and Protocols

  • Editors: Sanjoy K. Bhattacharya

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6996-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6995-1Published: 29 June 2017

  • Softcover ISBN: 978-1-4939-8362-9Published: 08 August 2018

  • eBook ISBN: 978-1-4939-6996-8Published: 28 June 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 281

  • Number of Illustrations: 33 b/w illustrations, 41 illustrations in colour

  • Topics: Biochemistry, general

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