Volume 4, issue 1, December 2004
33 articles in this issue
-
-
Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression
Authors (first, second and last of 5)
- Michael R Dyson
- S Paul Shadbolt
- John McCafferty
- Content type: Research article
- Open Access
- Published: 14 December 2004
- Article: 32
-
A sugar beet chlorophyll a/b binding protein promoter void of G-box like elements confers strong and leaf specific reporter gene expression in transgenic sugar beet
Authors
- Dietmar J Stahl
- Dorothee U Kloos
- Reinhard Hehl
- Content type: Research article
- Open Access
- Published: 05 December 2004
- Article: 31
-
Identification of proteins in laser-microdissected small cell numbers by SELDI-TOF and Tandem MS
Authors (first, second and last of 7)
- Grazyna Kwapiszewska
- Markus Meyer
- Ludger Fink
- Content type: Methodology article
- Open Access
- Published: 03 December 2004
- Article: 30
-
Over expression of the selectable marker blasticidin S deaminase gene is toxic to human keratinocytes and murine BALB/MK cells
Authors (first, second and last of 7)
- Fernanda Mara Bento
- Daniela Takeshita
- Sang Won Han
- Content type: Research article
- Open Access
- Published: 02 December 2004
- Article: 29
-
Single-track sequencing for genotyping of multiple SNPs in the N-acetyltransferase 1 (NAT1) gene
Authors (first, second and last of 6)
- Pavel Soucek
- Camilla Furu Skjelbred
- Vessela N Kristensen
- Content type: Methodology article
- Open Access
- Published: 25 November 2004
- Article: 28
-
Mucosal delivery of anti-inflammatory IL-1Ra by sporulating recombinant bacteria
Authors (first, second and last of 5)
- Stefano Porzio
- Paola Bossù
- Aldo Tagliabue
- Content type: Research article
- Open Access
- Published: 30 October 2004
- Article: 27
-
Efficient activation of gene expression using a heat-shock inducible Gal4/Vp16-UAS system in medaka
Authors
- Clemens Grabher
- Joachim Wittbrodt
- Content type: Methodology article
- Open Access
- Published: 26 October 2004
- Article: 26
-
Enhanced cell-permeant Cre protein for site-specific recombination in cultured cells
Authors (first, second and last of 4)
- Qing Lin
- Daewoong Jo
- H Earl Ruley
- Content type: Methodology article
- Open Access
- Published: 22 October 2004
- Article: 25
-
Detecting imbalanced expression of SNP alleles by minisequencing on microarrays
Authors (first, second and last of 4)
- Ulrika Liljedahl
- Mona Fredriksson
- Ann-Christine Syvänen
- Content type: Methodology article
- Open Access
- Published: 22 October 2004
- Article: 24
-
Use of polyethyleneimine polymer in cell culture as attachment factor and lipofection enhancer
Authors (first, second and last of 6)
- Ajith R Vancha
- Suman Govindaraju
- Rafael P Ballestero
- Content type: Methodology article
- Open Access
- Published: 15 October 2004
- Article: 23
-
Reduction of arsenic content in a complex galena concentrate by Acidithiobacillus ferrooxidans
Authors (first, second and last of 5)
- Mario Makita
- Margarita Esperón
- Erasmo Orrantia
- Content type: Research article
- Open Access
- Published: 13 October 2004
- Article: 22
-
A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods
Authors (first, second and last of 4)
- Justin C Yarrow
- Zachary E Perlman
- Timothy J Mitchison
- Content type: Methodology article
- Open Access
- Published: 09 September 2004
- Article: 21
-
Rapid and reliable extraction of genomic DNA from various wild-type and transgenic plants
Authors
- Tae-Jin Kang
- Moon-Sik Yang
- Content type: Methodology article
- Open Access
- Published: 02 September 2004
- Article: 20
-
Multi-line split DNA synthesis: a novel combinatorial method to make high quality peptide libraries
Authors (first, second and last of 4)
- Ichiro Tabuchi
- Sayaka Soramoto
- Yuzuru Husimi
- Content type: Methodology article
- Open Access
- Published: 01 September 2004
- Article: 19
-
Heterologous expression of plant virus genes that suppress post-transcriptional gene silencing results in suppression of RNA interference in Drosophilacells
Authors (first, second and last of 4)
- Brian Reavy
- Sheila Dawson
- Stuart A MacFarlane
- Content type: Research article
- Open Access
- Published: 25 August 2004
- Article: 18
-
A faster way to make GFP-based biosensors: Two new transposons for creating multicolored libraries of fluorescent fusion proteins
Authors
- Douglas L Sheridan
- Thomas E Hughes
- Content type: Methodology article
- Open Access
- Published: 18 August 2004
- Article: 17
-
Use and comparison of different internal ribosomal entry sites (IRES) in tricistronic retroviral vectors
Authors (first, second and last of 7)
- Victorine Douin
- Stephanie Bornes
- Bettina Couderc
- Content type: Research article
- Open Access
- Published: 27 July 2004
- Article: 16
-
Investigating the utility of combining Φ29 whole genome amplification and highly multiplexed single nucleotide polymorphism BeadArray™ genotyping
Authors (first, second and last of 12)
- Rebecca Pask
- Helen E Rance
- John A Todd
- Content type: Methodology article
- Open Access
- Published: 27 July 2004
- Article: 15
-
Two-fold differences are the detection limit for determining transgene copy numbers in plants by real-time PCR
Authors
- Ben Bubner
- Klaus Gase
- Ian T Baldwin
- Content type: Methodology article
- Open Access
- Published: 13 July 2004
- Article: 14
-
Quantitative promoter analysis in Physcomitrella patens: a set of plant vectors activating gene expression within three orders of magnitude
Authors (first, second and last of 5)
- Verena Horstmann
- Claudia M Huether
- Eva L Decker
- Content type: Research article
- Open Access
- Published: 07 July 2004
- Article: 13
-
Miniaturized fluorescent RNA dot blot method for rapid quantitation of gene expression
Authors (first, second and last of 5)
- Fekadu Yadetie
- Arne K Sandvik
- Astrid Laegreid
- Content type: Methodology article
- Open Access
- Published: 10 June 2004
- Article: 12
-
Optimization of ectopic gene expression in skeletal muscle through DNA transfer by electroporation
Authors (first, second and last of 7)
- Jordan Taylor
- Charlie F Babbs
- Kevin Hannon
- Content type: Methodology article
- Open Access
- Published: 18 May 2004
- Article: 11
-
Activation of the control reporter plasmids pRL-TK and pRL-SV40 by multiple GATA transcription factors can lead to aberrant normalization of transfection efficiency
Authors
- Clement KM Ho
- Jerome F Strauss III
- Content type: Research article
- Open Access
- Published: 30 April 2004
- Article: 10
-
A gene expression system offering multiple levels of regulation: the Dual Drug Control (DDC) system
Authors (first, second and last of 5)
- Marina Sudomoina
- Ekaterina Latypova
- Ilya G Serebriiskii
- Content type: Methodology article
- Open Access
- Published: 29 April 2004
- Article: 9
-
Rapid isolation of yeast genomic DNA: Bust n' Grab
Authors
- Susanna Harju
- Halyna Fedosyuk
- Kenneth R Peterson
- Content type: Methodology article
- Open Access
- Published: 21 April 2004
- Article: 8
-
Site-specific genomic (SSG) and random domain-localized (RDL) mutagenesis in yeast
Authors
- Misa Gray
- Martin Kupiec
- Saul M Honigberg
- Content type: Methodology article
- Open Access
- Published: 16 April 2004
- Article: 7
-
A large semi-synthetic single-chain Fv phage display library based on chicken immunoglobulin genes
Authors (first, second and last of 7)
- Wouter van Wyngaardt
- Teresiah Malatji
- Dion H du Plessis
- Content type: Research article
- Open Access
- Published: 01 April 2004
- Article: 6
-
Cell-based assay for the detection of chemically induced cellular stress by immortalized untransformed transgenic hepatocytes
Authors (first, second and last of 6)
- Maria Grazia Sacco
- Laura Amicone
- Marco Tripodi
- Content type: Methodology article
- Open Access
- Published: 19 March 2004
- Article: 5
-
The defH9-iaaM auxin-synthesizing gene increases plant fecundity and fruit production in strawberry and raspberry
Authors (first, second and last of 4)
- Bruno Mezzetti
- Lucia Landi
- Angelo Spena
- Content type: Research article
- Open Access
- Published: 15 March 2004
- Article: 4
-
The limits of log-ratios
Authors (first, second and last of 9)
- Vasily Sharov
- Ka Yin Kwong
- John Quackenbush
- Content type: Methodology article
- Open Access
- Published: 08 March 2004
- Article: 3
-
A new approach to 'megaprimer' polymerase chain reaction mutagenesis without an intermediate gel purification step
Authors
- Rajiv Tyagi
- Richard Lai
- Ronald G Duggleby
- Content type: Methodology article
- Open Access
- Published: 26 February 2004
- Article: 2
-
Recombining overlapping BACs into a single larger BAC
Authors
- George Kotzamanis
- Clare Huxley
- Content type: Methodology article
- Open Access
- Published: 06 January 2004
- Article: 1