Volume 3, issue 1, December 2003
23 articles in this issue
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A new reverse transcription-polymerase chain reaction method for accurate quantification
Authors
- Yih-Horng Shiao
- Content type: Methodology article
- Open Access
- Published: 09 December 2003
- Article: 22
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Expressing functional siRNAs in mammalian cells using convergent transcription
Authors (first, second and last of 4)
- Nham Tran
- Murray J Cairns
- Greg M Arndt
- Content type: Methodology article
- Open Access
- Published: 06 November 2003
- Article: 21
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Performance of high-throughput DNA quantification methods
Authors (first, second and last of 6)
- Kashif A Haque
- Ruth M Pfeiffer
- Andrew W Bergen
- Content type: Methodology article
- Open Access
- Published: 28 October 2003
- Article: 20
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Chemically defined medium and Caenorhabditis elegans
Authors
- Nathaniel J Szewczyk
- Elena Kozak
- Catharine A Conley
- Content type: Methodology article
- Open Access
- Published: 27 October 2003
- Article: 19
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Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions
Authors (first, second and last of 14)
- Frederique Ponchel
- Carmel Toomes
- Alex F Markham
- Content type: Methodology article
- Open Access
- Published: 13 October 2003
- Article: 18
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Quantitative Real Time Polymerase Chain Reaction for measurement of human Interleukin – 5 receptor alpha spliced isoforms mRNA
Authors (first, second and last of 8)
- Claudina Pérez
- Jo Vandesompele
- Claus Bachert
- Content type: Methodology article
- Open Access
- Published: 30 September 2003
- Article: 17
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In uterorecombinant adeno-associated virus gene transfer in mice, rats, and primates
Authors (first, second and last of 5)
- Deiadra J Garrett
- Janet E Larson
- J Craig Cohen
- Content type: Methodology article
- Open Access
- Published: 30 September 2003
- Article: 16
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In vivo mucosal delivery of bioactive human interleukin 1 receptor antagonist produced by Streptococcus gordonii
Authors (first, second and last of 11)
- Susanna Ricci
- Giovanni Macchia
- Diana Boraschi
- Content type: Research article
- Open Access
- Published: 17 September 2003
- Article: 15
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Coupling of importin beta binding peptide on plasmid DNA: transfection efficiency is increased by modification of lipoplex's physico-chemical properties
Authors (first, second and last of 4)
- Marie Carrière
- Virginie Escriou
- Daniel Scherman
- Content type: Research article
- Open Access
- Published: 12 September 2003
- Article: 14
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Gene expression analysis on small numbers of invasive cells collected by chemotaxis from primary mammary tumors of the mouse
Authors (first, second and last of 6)
- Weigang Wang
- Jeffrey B Wyckoff
- John S Condeelis
- Content type: Methodology article
- Open Access
- Published: 12 August 2003
- Article: 13
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An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography
Authors
- Christoph Scheich
- Volker Sievert
- Konrad Büssow
- Content type: Methodology article
- Open Access
- Published: 28 July 2003
- Article: 12
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Characterization of mutations and loss of heterozygosity of p53 and K-ras2 in pancreatic cancer cell lines by immobilized polymerase chain reaction
Authors
- James Butz
- Eric Wickstrom
- Jeremy Edwards
- Content type: Methodology article
- Open Access
- Published: 23 July 2003
- Article: 11
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Hydrodynamic guiding for addressing subsets of immobilized cells and molecules in microfluidic systems
Authors (first, second and last of 6)
- Thomas Brevig
- Ulrich Krühne
- Lars H Pedersen
- Content type: Methodology article
- Open Access
- Published: 23 July 2003
- Article: 10
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The Flp double cross system a simple efficient procedure for cloning DNA fragments
Authors
- Paul D Sadowski
- Content type: Methodology article
- Open Access
- Published: 18 July 2003
- Article: 9
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A fluorescent cassette-based strategy for engineering multiple domain fusion proteins
Authors
- Kevin Truong
- Ahmad Khorchid
- Mitsuhiko Ikura
- Content type: Methodology article
- Open Access
- Published: 15 July 2003
- Article: 8
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Expression of self-complementary hairpin RNA under the control of the rolCpromoter confers systemic disease resistance to plum pox virus without preventing local infection
Authors (first, second and last of 5)
- Tiziana Pandolfini
- Barbara Molesini
- Annalisa Polverari
- Content type: Research article
- Open Access
- Published: 25 June 2003
- Article: 7
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Oligodeoxyribonucleotide probe accessibility on a three-dimensional DNA microarray surface and the effect of hybridization time on the accuracy of expression ratios
Authors (first, second and last of 10)
- David R Dorris
- Allen Nguyen
- Abhijit Mazumder
- Content type: Research article
- Open Access
- Published: 11 June 2003
- Article: 6
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Efficiently folding and circularly permuted variants of the Sapphire mutant of GFP
Authors
- Otilia Zapata-Hommer
- Oliver Griesbeck
- Content type: Methodology article
- Open Access
- Published: 22 May 2003
- Article: 5
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In situtransduction of target cells on solid surfaces by immobilized viral vectors
Authors
- David A Hobson
- Mark W Pandori
- Takeshi Sano
- Content type: Methodology article
- Open Access
- Published: 09 May 2003
- Article: 4
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Crude extracts of bacterially expressed dsRNA can be used to protect plants against virus infections
Authors (first, second and last of 4)
- Francisco Tenllado
- Belén MartÃnez-GarcÃa
- José Ramón DÃaz-RuÃz
- Content type: Research article
- Open Access
- Published: 20 March 2003
- Article: 3
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Retrofitting BACs with G418 resistance, luciferase, and oriP and EBNA-1 – new vectors for in vitro and in vivodelivery
Authors (first, second and last of 5)
- Christine Magin-Lachmann
- George Kotzamanis
- Clare Huxley
- Content type: Research article
- Open Access
- Published: 03 February 2003
- Article: 2
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Disruption of vitellogenin gene function in adult honeybees by intra-abdominal injection of double-stranded RNA
Authors (first, second and last of 5)
- Gro V Amdam
- Zilá LP Simões
- Stig W Omholt
- Content type: Methodology article
- Open Access
- Published: 20 January 2003
- Article: 1