Highlights

  • The crude methanol extract of Piper guineense leaves was partitioned into dichloromethane (DCM), ethyl acetate (EtOAc), n-butanol (nBuOH) and aqueous fractions

  • Each fraction of Piper guineense was tested for antidiabetic and antioxidant activities

  • The identification and quantification of phytochemicals in ethyl acetate and n-butanol fractions was based on GC-MS analysis

  • The ethyl acetate and n-butanol fractions were potent inhibitor of α-glucosidase while only n-butanol fraction exhibited appreciable inhibition against α-amylase

  • None of the four fractions showed significant inhibitory activity towards dipeptidyl-peptidase-IV.

  • The n-butanol fraction showed the highest total antioxidant capacity while ethyl acetate fraction had the highest ferric reducing antioxidant power

  • The GC-MS analysis identified a total of 71 and 34 phytochemicals in n-butanol and ethyl acetate fractions respectively

  • Tributyl acetylcitrate (10.95%) and phytol (9.11%) were the major components in the n-butanol fraction while ethyl piperonyl cyanoacetate (27.35%) and phytol (15.17%) were the major constituents in the ethyl acetate fraction

Introduction

Diabetes mellitus is one of the major global public health problems of the twenty-first century affecting about 347 million people worldwide [1]. This problem is expected to double by the year 2030, if necessary actions were not taken [2]. This health disease is a chronic metabolic disorder caused by the total (or relative) defect of insulin, which is manifested clinically as elevated blood glucose. The diabetic patients exhibited a higher risk in the development of several chronic health complications including obesity, atherosclerosis, dyslipidemia and renal failure worldwide [3]. International Diabetes Federation [4] classified diabetes into clinical categories as: Type 1 Diabetes (T1DM), caused by β-cells destruction and usually leading to absolute insulin deficiency, Type 2 Diabetes (T2DM), due to progressive insulin secretory defect on the background of insulin resistance, and others such as gestational diabetes and specific type as monogenic diabetes syndromes, exocrine pancreas diseases, and drug or chemical induced diabetes.

In 2015, diabetes was one of the leading causes of non-communicable diseases (NCD) death, contributing 1.5 million deaths globally [5] and 321, 100 deaths [4] in the African region. The majority of people with diabetes are affected by Type 2 diabetes which is used to occur nearly entirely among adults, but now occurs in children too [6]. The high prevalence of Type 2 diabetes is as a result of aging, urbanization, and life styles changes [7]. The increasing prevalence of T2DM has stimulated the development of many new approaches to treat hyperglycemia in order to maintain glucose concentrations as close to normal as possible and to prevent the development of complications [8]. To date, many antidiabetic drugs of different classes and mechanisms of action are available as therapeutics for oral administration, including biguanides, sulfonylureas, meglitinides, PPAR-γ agoinsts, α-glucosidase inhibitors, DPP-4 inhibitors, SGLT2 inhibitors, dopamine-2 agonist; injectable therapies include GLP-1 agonists, amylin analogs, insulin and insulin analogues [9].

Despite the number of drugs available to control hyperglycemia and diabetes complication, there are concern regarding the side effects, availability and the cost of the drug in the middle- and low-income countries [10]. Hence, the research into other drugs that could better manage the treatment of diabetes is important and considering the invaluable biodiversity and countless number of metabolites present in plants have been a rich source in the search of new drugs [11]. Therefore, herbal prescriptions have received considerable attention as an alternative way to compensate for perceived deficiencies in orthodox pharmacotherapy [12].

Medicinal plants provide a potential source of hypoglycemic drugs because many plants and plant derived compounds have been used in the treatment of diabetes. Among these medicinal plants is Piper guineense, a West African spice plant from the family Piperaceae and genus piper. It is commonly called Ashanti pepper [13] and locally known as Uziza in Igbo, Masoro in Hausa and Iyere in Yoruba. Other common names are Benin pepper, Guinea pepper and false cubeb [13]. It is a perennial climbing plant that can grow up to 20 m tall by means of adventitious roots. It is widely consumed in Nigeria and Ghana on account of its nutritional and medicinal properties [14]. It is used in Western African cuisine because it contains piperine which imparts” heat” (piquantness) and a spicy pungent aroma to West African soup [13]. The proximate analysis of the plant revealed the presence of crude protein, fat, carbohydrate [15, 16], vitamins (A, B1, B2, C and E) [14,15,16] and minerals (Ca, Mg, K, Na, P and Fe) [14,15,16].

The phytochemical analysis of P. guineense leaves showed the presence of alkaloids (wisamine, dihydrowisamine, piperine and dihydropiperine) [17], saponins, tannins, flavonoids, resins [18], essential oil (dillapiol, elemicine, myristicine and safrole) [19] and hydrogen cyanides [20] which have a lot of pharmacological properties in diseases such as diabetes mellitus [21]. The components of essential oil exhibit bactericidal and antimicrobial effects on some micro-organisms [22] while large amount of beta-carophyllene in P. guineense has been investigated as an anti-inflammatory agent [23]. Similar to P. umbellatum, it is used in herbal medicine to treat rheumatism and arterial hypertension [24, 25]. Almost every part of the plant is used in forklore medicine to treat different diseases; for instance, the leaves are used to treat wounds, respiratory infections and syphilis [26], female infertility and low sperm count in male [27]. In China, P. guineense fruit extract is used in the treatment of epilepsy [28]. The seed is used as an adjuvant for the treatment of asthma [29] and weight control [30] while the root is used as chewing stick for healthy teeth [31].

P. guineense has been reported to be of value in the control of hyperglycemia in Type II diabetic Wistar rats [32, 33]. However, it is pertinent to further investigate molecular targets of P. guineense in diabetes. Thus, the present study sought to evaluate the inhibitory effects of different fractions of 80% methanol extract of P. guineense leaves on carbohydrate hydrolyzing enzymes and to identify phytochemicals in different fractions of P. guineense leaves extract.

Materials and methods

Chemicals and reagents

Yeast α-glucosidase, acarbose, p-nitrophenyl phosphate (pNPP), p-nitrophenyl α-D-glucopyranoside (pNPG), Pro-4-nitroanilide, Ascorbic acid, 3, 5 - Dinitrosalicylic acid, Dipeptidyl petidase IV were purchased from Sigma-Aldrich (St. Louis, MO, USA). The other chemicals and reagents used were of analytical grade.

Collection and identification of plant material

The fresh leaves of Piper guineense were purchased from Lanfenwa Market, Abeokuta, Ogun State. The leaves were authenticated by Mr. A.S. Oyelakin (Plant Taxonomist) at the Herbarium of Department of Pure and Applied Botany, Federal University of Agriculture Abeokuta, Ogun State with voucher number FUNAAB A0061.

Extraction and fractionation

The leaves were washed, air dried for 7 days and grinded into fine powder with an electric blender. Fifty gram (50 g) of the leaves was extracted with 250 ml of 80% methanol for 24 h. The methanol extract was then filtered with Whatman No.1 filter paper [34], the filtrate was concentrated under reduced pressure to remove methanol. Methanol was preferred because of its greater potential for extracting medicinal phytochemicals from their crude source [34]. The 80% methanol extract was then suspended in distilled water and then partition with ethyl-acetate (EtOAc), n-butanol (BuOH), Dichloromethane (DCM) and aqueous successively as previously described by Akinpelu et al., [35]. All the fractions were concentrated in rotary evaporator, air dried and stored in universal sample bottles [34, 35].

In vitro α-amylase inhibitory assay

This assay was conducted using the fraction of the sample as described using a modified procedure [36]. Test sample of 1 ml in 0.02 M sodium phosphate buffer (pH 6.9 with 0.006 M Sodium chloride) containing 1 ml of α-amylase (from Aspergillus oryzae) were pre-incubated at 25 °C for 30 min, after which 1 ml of 1% starch solution in 0.02 M sodium phosphate buffer (pH 6.9) was added after incubation of the reaction mixture at 25 °C for 10 min. The reaction was terminated by adding 1 ml of 3, 5- dinitrosalicylic acid (DNS) colour reagent (1.0 g of DNS, 20 ml of 2 M of NaOH and 30 g of Sodium potassium tartarate in 100 ml of distilled water). The sample test tubes were then incubated in boiling water for 5 min and cooled to room temperature. The reaction mixture was then diluted with 5 mL distilled water and the absorbance was measured at 540 nm using spectrophotometer. A control was prepared using the same procedure replacing the extract with distilled water. The generation of reducing sugar was quantified by reduction of 3, 5-dinitrosalicyclic acid to 3-amino-5-nitrosalicyclic acid. Acarbose was used as a positive control.

The α-amylase inhibitory activity was calculated as percentage inhibition:

$$ \%\mathrm{Inhibition}=\left[\left({\mathrm{Abs}}_{\mathrm{control}}-{\mathrm{Abs}}_{\mathrm{fractions}}\right)/{\mathrm{Abs}}_{\mathrm{control}}\right]\times 100 $$

Concentrations of fractions resulting in 50% inhibition of enzyme activity (IC50) were determined graphically.

In vitro α-glucosidase inhibitory assay

This assay was conducted using the fractions of P. guineense according to the method described by [37]. The substrate solution, p-nitrophenyl glucopyranoside (pNPG) was prepared in 20 mM phosphate buffer, and pH 6.9. 20 µL of α- glucosidase (0.2 U/mL) was pre-incubated with 20 µL of the different concentrations of the fractions for 10 min. Then 20 µL of 10.0 mM (pNPG) as a substrate dissolved in 20 mM phosphate buffer (pH 6.9) was then added to start the reaction. The reaction mixture was incubated at 37 °C for 15 min and stopped by adding 80 μL of 0.2 m of Na2CO3. The α-glucosidase activity was determined by measuring the yellow-colored para-nitrophenol released from pNPG at 405 nm with microplate reader. Control contained the same reaction mixture and the same volume of phosphate was added instead of sample solution. Acarbose was dissolved in 10% Dimethyl sulfoxide (DMSO) and used as a positive control. The inhibition percentage was calculated as follow;

$$ \%\mathrm{inhibition}\kern0.5em =\kern0.5em \frac{\left[\mathrm{Abs}\ \mathrm{control}\kern0.5em -\kern0.5em \mathrm{Abs}\ \mathrm{sample}\right]}{\left[\mathrm{Abs}\ \mathrm{control}\right]}\kern0.5em \times \kern0.5em 100 $$

Concentrations of fractions resulting in 50% inhibition of enzyme activity (IC50) were determined graphically.

In vitro dipeptidyl peptidase IV (DPP -IV) inhibitory assay

The assay was performed based on the method described by [38]. The assay was performed in micro well plates with 96 holes and read using a microplate reader. Vitagliptin as a standard of inhibitor DPP- IV was made in various concentrations (5.0, 2.5, 1.25, 0.625 mg/dl) in a 50 mM Tris HCl buffer (pH 7.5), the volume used was 35ul, The substrate was chromogenic GPPN (0.2 mM in Tris HCl buffer (pH 7.5), cleaved by DPP-IV a serine protease that releases paranitroanlide (pNA) a yellow coloured product which is measured at 380 nm. The enzyme solution of DPP-IV (0.05 units/ml) 15ul was added in micro well plates, and 35ul of the sample fraction with varying concentrations were then incubated for 15 min at 37 °C. Glacial acetic acid 25% (25ul) was added to the mixture to stop the enzymatic reaction; the absorbance was measured at 380 nm using the microplate reader. The total volume of the solution in the micro- well plate is 125 ml. The results were then compared with negative control (without inhibitor). Tests were performed in triplicates. One unit of enzyme activity was defined as the amount of enzyme that catalyzed the release of 1 μmol of pNA from the substrate per minute in the experimental conditions.

The percentage of inhibition was calculated using the following formula:

$$ \%\mathrm{inhibition}\kern0.5em =\kern0.5em \frac{\left(\mathrm{absorbance}\ \mathrm{of}\ \mathrm{control}\kern0.5em -\kern0.5em \mathrm{absorbance}\ \mathrm{of}\ \mathrm{inhibitor}\right)}{\mathrm{Absorbance}\ \mathrm{of}\ \mathrm{control}}\kern0.5em \times \kern0.5em 100 $$

Total antioxidant capacity

The total antioxidant capacity was determined by phosphomolybdate method using ascorbic acid as standard [39]. An aliquot of 25ul of each (1 mg/ml) solution was mixed with 300ul of TAC reagent solution (0.6 M sulphuric acid, 28 mM sodium phosphate and 4 mM ammonium molybdate). The micro plate was incubated in a micro plate incubator for 90 min. The absorbance of the mixtures was measured at 630 nm in a micro plate reader. The antioxidant activity of the extract was expressed as the number of mg equivalents to ascorbic acid (AAE)/g extract. All the experiments were carried out in triplicates.

Ferric reducing antioxidant power

The ferric reducing antioxidant power of the fractions was evaluated according to the method of [40]. An aliquot of 25ul of each extract (1 mg/dl) was mixed with 300ul of FRAP reagents (300 mM Acetate buffer, 0.03 g of TPTZ (2,4,6-Tris(2-pyridyl) 3- triazine) in 1 ml of 40 mM HCL and 18.6ul of 20 mM ferric chloride in 5 ml of water in ratio 10:1:1). After incubation for 90 min at 37 °C, the absorbance was measured at 593 nm in a micro plate reader. The assay was carried out in triplicates. And the antioxidant activity of the extract was expressed as the number of mg equivalents of ascorbic acid (AAE)/g extract.

Gas chromatography - mass spectrometry (GC-MS) analysis

The leaves fractions were subjected to GC-MS analysis carried out at the Federal Institute of Industrial Research, Oshodi (FIIRO), Lagos State, Nigeria. This method was used for the identification of biologically active natural chemical constituents [41]. It was carried out by using GC-MS-5975C Agilent system composed of an auto sampler and a gas chromatograph interfaced with a mass spectrometer (GC-MS) instrument. It was worked with the following conditions using a fitted variant column Agilent JSW HP- 5 MS (30 m × 0.32 mm × 0.25 μM), sample was dissolved in hexane and 1 μL inject automatically into the column with the injector temperature set at 250 °C. Helium gas was used as the carrier gas at a flow rate of 1 ml/min. GC oven temperature was started at 50 °C held for 5 mins and then raised to 80 °C at the rate of 5 °C / min for 9 min. The temperature of injector and ion source were maintained at 250 °C and 280 °C respectively. For the GC-MS analysis an injector was done on a duplicate of the same operational conditions. The mass spectrum of compounds in sample was obtained by electron ionization at 7 eV, ion source temperature was 230 °C and interface temperature 250 °C and its detector was operated in scan mode from 45 to 450 amu (atomic mass units). A scan interval of 0.5 s and fragment from 45 to 450 amu was maintained. The total running time was 60 mins.

Identification of chemical constituents

The chemical compounds were identified by comparing the spectral data obtained on the Gas Chromatograph Mass Spectroscopy with the data base of National Institute Standard and Technology Library and that of Wiley GC-MS library and confirmed by comparison of their retention indices measured index (Ki) to a homologous series of n-alkanes. The name of the chemical component, molecular weight and the chemical structure of the fraction of the leaves of Piper guineense were identified.

Statistical analysis

All the assays were done in triplicate. Means, Standard Error of Means (SEM) and IC50 values were calculated using a Microsoft excel program and Graph Pad® prism 6.0. The test of significance was done using Student’s t-test (two-tailed). Replicate values for each treatment were compared with replicate values of the control; and values considered significant at p˂0.05.

Results

Percentage yield of different solvent fractions of P. guineense

The percentage yield of the different solvent fraction is as shown in Table 1. The highest yield was obtianed from the n-butanol fraction which gave value of 3.16%. This was followed by the DCM fraction with a yield of 2.22%. the aqueous fraction showed a yield of 0.68% while the least yield was obtained with the ethyl acetate fraction (0.66%).

Table 1 Percentage yield of different solvent fractions of 80% methanol extract of P. guineense
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The in vitro inhibitory effect of solvent fractions of P. guineense on α-amylase

The in vitro inhibitory effect of the solvent fractions from the 80% methanol leaf extract of P. guineense towards α-amylase activity is as shown in Table 2. The result indicated that the n-BuOH fraction exhibited a significant (p˂0.05) inhibition on the α-amylase activity with an IC50 value of 0.33 ± 0.09 as compared to acarbose that elicited an inhibitory activity of 1.09 ± 0.19 μg/ml. The other fractions exhibited activity significantly (p˂0.05) lower than acarbose.

Table 2 In vitro inhibitory aactivities of different solvent fractions of P. guineense on α amylase
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The α-glucosidase inhibitory effect of solvent fractions of P. guineense

The in vitro inhibitory effect of the solvent test fractions on the activity of α-glucosidase is as shown in Table 3. The highest inhibitory effect towards α-glucosidase was observed with the ethyl acetate fraction with an IC50 of 0.24 ± 0.07 μg/ml. This was followed by n-butanol with an IC50 of 0.83 ± 0.15. The IC50 values of ethyl acetate and n-butanol fractions were significantly (p˂0.05) lower than acarbose, the positive standard, which elicited an IC50 value of 1.46 ± 0.21 μg/ml. The DCM and aqueous fractions showed weak effect on α-glucosidase while it could be observed that ethyl acetate and n-BuOH fractions were more effective than the positive standard.

Table 3 In vitro inhibitory effects of different solvent fractions fractions of P. guineense on α- glucosidase activity
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The in vitro DPP-IV inhibition of DPP-IV by solvent fractions of P. guineense

The result of the inhibitory effects of the different solvent fractions of P.guineense on dipeptidyl peptidase –IV activity is as shown in Table 4. It was observed that none of the fractions elicited any significant activity when compared to the positive standard (vidagliptin) used as control which gave an IC50 value of 0.07 ± 0.01 μg/ml.

Table 4 In vitro inhibitory effects of different solvent fractions of P.guineense on the activity of dipeptidyl peptidase –IV (DPP-IV)
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Ferric reducing antioxidant power (FRAP) of different solvent fractions of P. guineense

The result of the ferric reducing antioxidant power (FRAP) of the four solvent fractions obtained from the 80% methanol extract is as shown in Table 5. It was observed that the ethyl acetate fraction elicited the highest antioxidant power with a value of 53.96 ± 3.40 mg AAE/g followed by the dichloromethane fraction (DCM) fraction with a value of 47.52 ± 0.63 mg AAE/g of P. guineense while the least FRAP activity was observed for the aqueous fraction with a value of 16.98 ± 2.10 AAE/g.

Table 5 Ferric reducing antioxidant power (FRAP) activities of different solvent fractions of P. guineense
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Total antioxidant capacity (TAC) of different solvent fractions of P. guineense

The result of the Total antioxidant capacity of the four solvent fractions obtained from the 80% methanol extract is as shown in Table 6. It was observed that the n-butanol fraction exhibited the highest total antioxidant capacity of 9.98 ± 0.15 mg AAE/g of P.guineense while the least TAC activity was observed for dichloromethane fraction with a value of 6.19 ± 0.10 AAE/g.

Table 6 Total antioxidant capacity (TAC) activities of different solvent fractions of P. guineense
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Chemical composition of the n-butanol fraction of P. guineense

The analysis by GC-MS of the n-butanol fraction obtained from the 80% methanol extract of P. guineense is as shown in Table 7. The analysis showed that Seventy-one (71) compounds were detected in the n-butanol fraction. The compound tributyl acetylcitrate with a composition of 10.95% was the major constituent. This was followed by phytol with a composition of 9.11% and 9,10-Dimethyltricyclo [4.2.1.1(2,5)] decane with a composition of 6.74%. Other compounds identified with compositions above 2% include; 2-Butenal, 2-methyl-4-(2,6,6-trimethyl-1-, (2.88%), 1-Cyclohexane-1-methanol alpha,2,6,6, (4.26%), 3-Buten-2-one, 4-(4-hydroxy-2,6,6-trimethyl (4.26%), dibutyl phthalate (3.48%), hexanedecanoic acid (3.09%). All the other compounds had compositions that were less than 2%.

Table 7 Chemical composition of the n-butanol fraction of P. guineense following GC-MS analysis
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Chemical composition of the ethyl acetate fraction of P. guineense

Analysis of the ethyl acetate fraction obtained from the 80% methanol extract of P. guineense by GC-MS is as shown in Table 8. The analysis shows that thirty-four (34) compounds were detected in the ethyl acetate fraction. Ethyl piperonyl cyanoacetate and phytol were identified as the major compounds in the fraction with percentage compositions of 27.35 and 15.17% respectively. Other constituents include piperine (5.36%), benzene 3-iodo-1-methoxy-1 methylpropyl (8.65%),(R)-(−)-14-Methyl-8-hexadecyn-1-ol (6.88%), Octacosane (4.08%). All the other compounds had compositions that were less than 2%.

Table 8 Chemical composition of the ethyl acetate fraction of P. guineense following GC-MS analysis
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Discussion

Considering the severity of diabetes and its complications, any new therapeutic innovation is of interest to prevent deleterious effects of hyperglycemia. Anti-diabetics agent from natural sources is vital research most especially considering the side effects and high cost of most of the available synthetic drugs. In this study, different soluble fractions of P. guineense were investigated against α-amylase, α-glucosidase and DPP-IV activities inhibitory assay. The P. guineense leaves were fractionated into Aqueous, Butanol (BuOH), ethyl acetate (EtOAc), dichloromethane (DCM) and the percentage yield were 0.68, 3.16, 0.66 and 2.22 respectively. The difference in polarities of the partition solvents might influence the solubility of the chemical constituents of the sample and its extraction yield; this might be the reason for the variation in percentage yield.

One of the therapeutic approaches for treating type II diabetes is to decrease postprandial hyperglycemia and mainly two carbohydrate hydrolyzing enzymes (α-amylase and α-glucosidase) are responsible for postprandial hyperglycemia. The enzyme, α-amylase, begins the process of carbohydrate digestion by hydrolyzing of 1, 4-glycosidic linkages of polysaccharides (starch, glycogen) to disaccharides and α-glucosidase catalyze the disaccharides to monosaccharide, which leads to postprandial hyperglycemia [42]. Hence, inhibitors of the carbohydrate hydrolyzing enzymes are useful in the control of hyperglycemia as they delay the carbohydrate digestion but do not prevent the absorption of ingested carbohydrates, reducing the postprandial glucose and insulin peak. Thus, the inhibitory effect of different fractions of Piper guineense on the carbohydrate hydrolyzing enzymes were investigated, all the fractions showed different level of inhibitory activities against α-amylase enzyme over the range of 0.15626–5 mg/ml and IC50 (fraction concentration that inhibits the 50% enzyme activity) values of 2.10, 0.33, 5.75, 6.70 mg/ml were obtained for aqueous, BuOH, EtOAc, DCM and EtOAc fractions, respectively. BuOH fraction had the strongest activities compared to others and its percentage inhibition increase as concentration increases (concentration dependent). The BuOH fraction showed three times more potent α-amylase inhibitory activity than the acarbose, a commercially used α-glucosidase inhibitor that has IC50 value of 1.09 mg/ml.

Moreover, the fractions showed different inhibitory activities against the α-glucosidase enzyme with the IC50 values of 2.90, 0.83, 1.91 and 0.24 mg/ml for aqueous, BuOH, DCM and EtAOc respectively. The EtAOc fractions showed six times more potent α-glucosidase inhibitory activity, while BuOH fraction showed two times inhibitory activity compare to the acarbose. The difference in strong inhibitory activities of BuOH and EtAOc fractions was resulted from the differential distributions of the active compounds in the fractions.

Preventing the degradation of incretin hormones by DPP-IV inhibition became the attractive therapeutic strategy because several DPP-IV inhibitors have consistently reduced blood glucose predominately postprandial and this appears to be associated with increases in active circulating GLP-1 [43]. The mechanism of DPP-IV inhibitors is to increase incretin level (GLP-1 and GIP), which inhibit glucagon release, which in turn increases insulin secretion, decreases gastric emptying [44]. In the DPP-IV inhibitory assay, all the fractions showed concentration dependent inhibition (the % inhibition increases as the concentration increases) activities against DPP-IV over the range of 0.15625-5 mg/ml with IC50 values of 4.60 ± 0.87, 3.22 ± 0.03, 1.83 ± 0.35, 1.45 ± 0.12 mg/mL obtained for Aqueous, BuOH, DCM and EtOAc respectively. The EtOAc fraction was the most potent among the fractions with moderate activity against the DPP-IV. The action of EtOAc fraction showed that it has the ability to protect the incretin from degradation by the DPP-IV to metabolites that are devoid of insulin releasing activity.

Fe (III) reduction is often used as an indicator of electron donating activity, which is an important mechanism of phenolic antioxidant action [45, 46]. The reducing power ability (FRAP) of a compound generally depends on the presence of the reductones (antioxidants), which exert the antioxidant activity by breaking the free radical chain by donating a hydrogen atom [47]. Table 4 depicts the reductive capabilities of various fractions of Piper guineense. The FRAP activities showed that the reducing power ability of the fraction decreased in the order EtAOc > DCM > BuOH > AQUEOUS with value of 53.96 ± 0.40, 47.52 ± 0.63, 33.47 ± 0.79 and 16.98 ± 0.06 mgAAE/g ± SEM respectively. The antioxidant present in the fraction caused reduction of Fe3+/ferricyanide complex to the ferrous form and thus proved the reducing power ability. The EtOAc fractions showed good reducing power capacity which served as a significant indicator of its antioxidant activity [48].

The TAC is based on the reduction of molybdenum hexavalent oxidation state Mo (VI) to molybdenum pentavalent Mo (V) by the effect of electron donor by the antioxidant and formation of molybdenum complex colored green. The TAC of the fractions decreased in the order BuOH>Aqueous, EtOAc>DCM with the values 9.98 ± 0.15, 9.72 ± 0.02, 7.68 ± 0.17 mg, 6.19 ± 0.10 AAE/g ± SEM respectively.

The significant inhibition of α-glucosidase and α-amylase by n-butanol fraction as well as strong inhibitory effect of ethyl acetate fraction against α-glucosidase indicates that these fractions of 80% methanol extract of P. guineense leaves could serve as therapeutic approach in the management of type II diabetes and some bioactive compounds in both fractions posses interesting structure-function benefits with promising potentials. Phytol, one of the major constituents in both n-butanol and ethyl acetate fractions of P. guineense leaves extract in the present study, has been reported to show antidiabetic and antioxidant activity in alloxan induced diabetic rats at low doses [49]. Antidiabetic activity of couramanone [50], phthalic acid and derivative of phthalate [51] present in both P. guineense fractions has also been reported. The antioxidant activity of P. guineense could be attributed to the presence of phenolic compounds [31] in n-butanol fraction as well as piperine and its derivatives in ethyl acetate fraction [52]. The present study corroborates the findings of Agbor et al., [53] who reported the antioxidant activity of P. guineense.

GCMS analysis of plant extract has been found to be one of the most powerful tools that are useful for identifying chemical constituents of plants [54]. The EtOAc and BuOH fractions of Piper guineense were analyzed by GC-MS to detect various compounds, a total of seventy-one and thirty-four compounds were identified in BuOH and EtOAc fractions of Piper guineense respectively. Recent study conducted by Usman et al., [55] indicated that the component extracted from plant parts depends on the type of solvent. The major constituents of n-butanol fraction of P. guineense are tributyl acetylcitrate, phytol, 9, 10-Dimethyltricyclo [4.2.1.1(2, 5)] decane, 2-Butenal, 2-methyl-4-(2,6,6-trimethyl-1-, 1-Cyclohexane-1-methanol alpha,2,6,6, 3-Buten-2-one, 4-(4-hydroxy-2,6,6-trimethyl, dibutyl phthalate, hexanedecanoic acid while ethyl piperonyl cyanoacetate, phytol, piperine, benzene 3-iodo-1-methoxy-1 methylpropyl, (R)-(−)-14-Methyl-8-hexadecyn-1-ol, Octacosane were majorly identified in ethyl acetate fraction of P. guineense leaves. The activity of the fractions might be attributed to the combined action of the major compounds identified in P. guineense leaves extract.

Ojinnaka et al., [56] identified 22 peaks from the GCMS spectrum of ethanol extract of Piper guineense leaves and found that the spectrum was dominated by acids and hydrocarbon while alcohol and ester were the least constituents. The composition of 2 samples of fruit oil of P. guineense from Cameroon was dominated by linalool, β-caryophyllene, limonene, and β-pinene [57]. Germacrene B and limonene were the most abundant compounds of the essential oil of P. guineense leaves [57, 58]. Similar components were reported in ethanol extract of P. guimeense leaves and seeds [16]. A sample of fruit oil of Nigerian origin contained linalool as major component [59]. In São Tomé, the essential oil from aerial parts mainly consists of phenyl propanoid derivatives: dillapiole and myristicine [60]. Imeh and Onyeije [61] identified 6-dimethyloxazolo(5,4-c)pyridazin-4-amine, 3-(1-methylethyl)-cyclohexene, 4-methoxy-N-(4-nitrobenzyl)-benzamide, alpha bisabolene, 1,2,3,4,4a,5,6,8a-octahydro-7-methyl-4-methylene-1-(1-methylethyl)-napthalene, 3,7-diacetamidophenoxathin, 1,3,3-trimethyl tricyclo{2,2,1]heptane, 3H-indazol-3-one, 1H-indene,octahydro-1,7a-dimethyl-4-(1-methylethenyl)-1,4-methano-1H-indene, piperidine, 2,4-disopropenyl-1-methyl-1-vinyl, n-hexadecanoic acid, eudesdma-4 [14],11-diene as the major components in acetone extract of P. guineense leaves. It is worth noting that n-butanol and ethyl acetate fractions of 80% methanol extract of P. guineense leaves in the present study contain constituents that are not visible with other solvents.

Conclusion

The present study investigated the antioxidant properties and antidiabetic potential of four different solvent fractions of P. guineense through α-amylase, α-glucosidase, and DPP-IV inhibitory activity. It can be inferred from the results of the study, that among the fractions, the ethyl acetate and n-butanol fractions exhibited the best antioxidant and antidiabetic potentials. Thus further investigation on the bioactive compounds eliciting these properties is imperative in search for lead compounds from natural products that could be developed into a drug for the management of diabetes mellitus. In this regard, the use of in silico approach to carry out molecular docking experiments on the compounds profiles from the two fractions against suitable molecular targets relevant in glucose metabolism can be suitable approach. Furthermore, in vitro studies using the appropriate cell lines /tissue culture investigation should also be carried out to further elucidate the probable mechanism of action of constituents of P.guineense.