Background

Two significant challenges face mankind in the near future. The first is to feed a human population that may soon exceed nine billion people [1]; the second is to deal with increasing medical and veterinary problems arising from the emergence of antimicrobial-resistant (AMR) pathogenic bacteria [2]. Ruminant livestock production is highly relevant to both issues. Ruminants consume feedstuffs, including forages and by-products that cannot be used by non-ruminants. Thus, ruminant products provide high-quality nutrients from materials that do not compete with human-edible food. However, the ruminant digestive tract contains AMR genes that may be a reservoir from which AMR develops and spreads [2,3,4]. The use of antimicrobial feed additives increases the abundance of AMR genes in the gut and faeces [4, 5], particularly in beef cattle [3, 6]. Indeed, more antimicrobials are used in livestock production than in human therapeutics [7]. AMR genes in the ruminant gut are not entirely due to antimicrobial use, but appear also in non-medicated animals [8, 9]. Given the overarching importance of diet in determining the composition of the ruminal microbiome [10, 11], we considered that the absence of knowledge about the influence of diet on the ruminal “resistome” (the collection of all identified antibiotic resistance genes) was a deficiency in understanding how AMR develops and spreads in livestock. A diet change (e.g. forage to concentrate) could generate a stress for the rumen microbiome community [12] as similarly found in humans by Brown et al. [13]. Thus, we analysed metagenomics data from beef cattle which had previously been used to explore links between the host animal, its microbiome/metagenome and methane emissions and feed efficiency.

Dietary changes could also potentially initiate a “bloom” of specific microbial populations, mostly Proteobacteria [14], or enhance the abundance of stress-response genes within the microbial community [15]. This unbalanced microbial community, also known as “dysbiosis”, is generally observed after diet changes and alterations in ruminal volatile fatty acid (VFA) composition also potentially associated with a lower ruminal pH, antibiotic treatment, presence of heavy metals or toxic substances or infection with pathogenic bacteria [13, 16]. For example, a low acetate to propionate (A:P < 2.2) ratio is associated with a lower ruminal pH in animals offered a high-concentrate diet [17]. All of these factors can have negative effects on the overall health and production performance of cattle [18, 19]. Proteobacteria is one of the four main phyla in the rumen microbiome together with Firmicutes, Bacteroidetes and Actinobacteria. This phylum is composed of many pathogenic bacteria such as Escherichia coli, and the abundance of some of these opportunistic pathogens is sensitive to dietary change [20]. Diet has been shown to have a dominant effect on the shedding of the zoonotic pathogen, E. coli O157, from beef cattle [21]. Thus, the combined effects of pathogen abundance and the abundance and diversity of AMR genes have fundamental implications for human health.

Finally, microbial mechanisms including quorum sensing and fucose sensing [22, 23] are recognised as inter-kingdom signalling pathways [24] and help to regulate bacterial colonisation and virulence within the host [25].

Our study design allowed us to test the impact of diet (“concentrate” versus “forage”) and host influence (e.g. breed, age or weight) on the ruminal microbiota and functional genes associated with the microbiome [26]. Metagenomics was already confirmed as a good strategy to identify links between the rumen microbiome and host response [20, 27].

Therefore, the overall aim of our work was to improve knowledge on the diversity and importance of resistance- and pathogenicity-related genes as well as microbiome-host interaction genes in the rumen microbial community affected by diet treatments and over three independent trials. The factors influencing the composition of these genes in the rumen microbiome were evaluated, and the genes showing significant differences were identified. We hypothesised that the microbiome of cattle offered a concentrate-based diet will favour stress associated with acidotic challenge and therefore enriched in microbial genes related to the resistome or pathogenicity activities, when compared to the cattle offered the forage-based diet. Rumen samples with higher Proteobacteria content, reaching a dysbiosis state, were of particular interest for understanding its association with diet treatments and how this can be explained by enrichment of the targeted genes. We also tried to identify a possible link between these genes and the control that the host has on its microbiome. This could be an important step towards identifying novel biomarkers of possible host-microbiome interactions involving beneficial and detrimental effects on the health of the host and related to diet change.

Methods

Animals, experimental design and diets

In our previous study [26, 28], data on feed efficiency and methane emissions (measured using respiration chambers) were obtained from a 2 × 2 factorial design experiment of breed types and diets using 72 steers from a two-breed rotational cross between Aberdeen Angus (AA) and Limousin (LIM) and completed in 2011. Similar experiments were repeated using purebred Luing (LU) and crossbred Charolais (CH) steers in 2013 and Aberdeen Angus (AA) and Limousin (LIM) rotational crossbred steers in 2014. Prior to start of the experiment, all animals received the same diet type (forage-based diet) and thereafter were adapted to the experimental diets over 5 weeks. During the same period, they were also acclimatised to the group-housed environment and the electronic feeders (HOKO, Insentec, Marknesse, The Netherlands). Due to EU legislation, the application of antibiotics is prohibited for enhancing growth. In exceptional cases, animals were treated with antibiotics and then excluded from the trial. Finally, there was no change in diets of the animals and was no fasting period before the animals went to slaughter.

The data in this study were samples from those experiments whereby animals with extreme high and low methane emissions (2011) or feed conversion efficiency (2013 and 2014) were selected for whole genome sequencing. The breed type of the sampled progenies were balanced within experiment comprising of 4 AA and LIM in 2011, 9 LU and CH in 2013 and 12 AA and LIM in 2014. The animals were offered two complete diets ad libitum containing (g/kg DM) approximately 500 forage to 500 concentrate or 80 forage to 920 concentrate subsequently referred to as forage and concentrate diets, respectively (Additional file 1: Table S1). Full details of diets are given in Rooke et al. [11] and Duthie et al. [29, 30]. Animals from the 2014 experiment were only offered the forage diet (see Additional file 1: Table S1), and this experiment was included in our analysis to increase observations for the forage diet, which is more heterogeneous in composition than the concentrate diet and to reinforce the microbiome results obtained with similar forage diets for the 2011 and 2013 experiments. A single sample of rumen fluid for VFA analysis (expressed as molar proportions) was taken by stomach tube within 1 h of cattle leaving the respiration chambers in the 2011 experiment [11]. VFA were determined for 2013 and 2014 in samples collected directly at the abattoir [11]. As recommended by Terré et al. [31], we compared VFA profiles between samples rather than total VFA concentrations because of the different methods for rumen sampling applied. The acetate-to-propionate ratio was calculated and considered as a proxy for rumen pH, accepting that whilst the relationship between the two is generally strong, it is not exactly linear. A total of 50 animals including the 8 animals already studied in Roehe et al. [26], selected and balanced for breed type and diet, were used for further analyses (Additional file 1: Table S1). Post-mortem digesta samples were used here, following our previous discovery that the abundance of archaea relative to bacteria was similar between live and slaughter cattle [32].

Genomic analysis

As previously described in Roehe et al. [26], the animals were slaughtered in a commercial abattoir where two rumen fluid samples (approximately 50 mL) were taken immediately after the rumen was opened to be drained. DNA was extracted from the rumen samples following the protocol described in Rooke et al. [11].

Illumina TruSeq libraries were prepared from genomic DNA and sequenced on Illumina HiSeq systems 2500 (2011) and 4000 (2013, 2014) by Edinburgh Genomics. Further analyses using the two sets of data followed the same procedure as previously described in Roehe et al. [26]. Briefly, microbial functional genes encoding for proteins and including the genes detailed in this study were identified using the KEGG genes database. Genes with a relative abundance of more than 0.001% were selected for further analysis.

A specific analysis was carried out on antimicrobial resistance genes and a comparison between the annotation obtained using KEGG genes and the genotyping tool SRST2 associated with the ARG-ANNOT database was performed; the latter was created specifically to study AMR genes [33, 34]. ARG-ANNOT targets all AMR genes including those on the chromosome and offers the possibility to identify putative new AMR genes in bacterial genomes as well as the genes involved in regulatory function associated with the AMR genes [35] and still showing high sensitivity and specificity for these genes, similar to other databases (e.g. ResFinder database) [36].

As for the analysis of metagenomics data using KEGG, a matrix was generated using the depth values and normalising the relative abundance of each AMR genes per sample. The length and coverage values for AMR genes were on average 1386 ± 26 bp and 99.31 ± 0.08% respectively.

For 16S rRNA gene analysis, the genomic reads were aligned to the GREENGENES database [37] using Novoalign (www.novocraft.com). The Proteobacteria ratio was calculated as the abundance of Proteobacteria sequences divided by the sum of the abundance of Firmicutes and Bacteroidetes sequences and is used as indicator for microbial disorder in both the rumen of cattle and human intestines [12, 14]. Values equivalent or above 0.19 were considered as an indication of the microbial disorder also known as dysbiosis. This cut-off value was calculated based on the abundance of the bacterial phyla identified in the core rumen microbiome of cattle fed forage or concentrate diets [12] and confirmed as a marker of microbial dysbiosis in the human gut [14].

Parameters were adjusted such that all hits were reported that were equal in quality to the best hit for each read, and allowing up to a 10% mismatch across the fragment. Further details are included in Roehe et al. [26].

Statistical analysis

Statistical analysis of the metagenomic data was based on the complete sample profiles as expressed by the pattern of metagenomic operational functional units (OFUs) and the relative abundance (percentage) of individual OFU in each profile. Principal coordinate analysis (PCoA) and canonical variate analysis (CVA) were carried out using Gen-Stat 16th edition (VSN International Ltd., UK) to identify the factor(s) explaining the difference observed in microbial functional genes between samples. In CVA, each factor was tested individually in order to get a more constrained analysis.

Microbial functional gene abundances were compared individually or within a functional group (e.g. all genes associated with antibiotic resistance) using a general linear model with adjustment for multiple comparisons using the Bonferroni correction (SPSS Statistics 22, IBM, USA). Spearman’s correlations were carried out using SPSS Statistics 22 (IBM, USA) to identify the correlation between the AMR gene groups and Proteobacteria phylum or ratio, as well as between the Proteobacteria ratio and 20 Proteobacteria families or genera. P values ≤ 0.05 were considered significant and tendencies were reported (P values < 0.1).

In a network analysis using BioLayout Express3D [38], we identified the distinct functional clusters of microbial genes for each experiment and the combination of the three experiments. These networks consist of nodes representing microbial genes and the connecting edges determining the correlations in abundance and functional linkages between these genes.

We used partial least squares (PLS) analysis (Version 9.1 for Windows, SAS Institute Inc., Cary, NC, USA) to identify the most important genes associated with the Proteobacteria:(Firmicutes + Bacteroidetes) ratio. The PLS analysis accounts for multiple testing and the correlation between microbial genes. In addition to microbial genes, the model included the effects of diet (potentially stressor) and breed type (host genetics effect). The model selections were based on the variable importance for projection (VIP) criterion [39], whereby microbial genes with a VIP < 0.8 contribute little to the prediction.

Results

Major factors influencing functional genes in the microbiome

Two hundred and four genes associated with resistance, colonisation, communication or pathogenicity functions were identified from 4966 metagenomic genes using KEGG identification. These genes represent on average 1.07% of the total microbial genes in the rumen samples and could be classified into 20 functional groups (Additional file 1: Figure S1). The total number of selected genes was significantly higher (P = 0.046) in rumen samples from concentrate-fed compared to forage-fed cattle (Additional file 1: Figure S2A), and diversity was also greater in concentrate-fed steers (Additional file 1: Figure S2B). Biofilm formation and antibiotic resistance genes, representing 46.76% of the 204 genes, were the dominant groups detected. Other genes such as those encoding for quorum sensing only represented 0.1% of the 204 genes initially selected. Differences in gene composition observed between rumen samples and their clustering due to diets were confirmed by PCoA that explained 68.6% over the first two axes (Fig. 1a). After grouping the samples, CVA confirmed that diet was the only factor that was significantly correlated (no overlapping within the 95% circles of confidence) with the functional gene composition and explaining this difference (Fig. 1b). Host breed effect and Proteobacteria ratio were not significantly correlated with functional gene composition (Additional file 1: Figure S3).

Fig. 1
figure 1

a Principal Coordinates analysis (PCoA) of the structure of 204 selected genes (number of animals, n = 50 samples). Black cross: concentrate samples from 2011 experiment, white cross: concentrate samples from 2013 experiment, black triangle: forage samples from 2011 experiment, dark grey triangle: forage samples from 2013 experiment, grey triangle: forage samples from 2014 experiment. b Canonical variate analysis (CVA) of the structure of 204 selected genes (n = 50) based on diet. Black cross: samples from concentrate-fed animals (all years), grey triangle: samples from forage-fed animals (all years). Circle: 95% confidence range

Full size image

The abundance of 50 genes, each representing at least 0.001% of total genes per sample, was identified as different between diets (P < 0.05; Fig. 2). A greater number of genes (n = 32) were more abundant in samples from animals offered the concentrate diet (n = 18). Opposite results were observed between genes with similar function like AMR genes (Fig. 3). For example, genes associated with resistance to macrolide (K08217) and β-lactamase (K01467, K07576) were significantly more abundant in samples from animals offered the concentrate diet (P < 0.001), whilst the sum of genes including some specific genes related to chloramphenicol (K00638) and microcin resistance were dominant in forage-fed samples (P < 0.001; Fig. 3). Finally, genes encoding for aminoglycoside (K00897) and streptomycin (K00984) resistance were more abundant in concentrate treatment samples (P < 0.01; Figs. 2 and 3). In general, similar results were confirmed when using the more specific AMR genes database. The only difference was the identification of genes encoding for tetracycline resistance instead of chloramphenicol resistance, which were in general significantly more abundant (P < 0.01) in forage-fed animals (Additional file 1: Table S2 and S3).

Fig. 2
figure 2

Relative abundance of genes significantly different between diet treatments (n = 50). Mean values with standard error are presented. Grey: samples from forage-fed animals, dark grey: samples from concentrate-fed animals. Arrow indicates the genes that are also detected in the network analysis

Full size image
Fig. 3
figure 3

Diversity of AMR genes between diets (n = 50). AMR genes with similar antibiotic resistance are grouped together into a final number of 8 groups

Full size image

The relative abundance of genes associated with stress responses including oxidative stress and cold shock protein (K03704) differed between diets. For example, K03704 was threefold more abundant with the concentrate diet. The concentrate diet enhanced the relative abundance of genes associated with motility, attachment to host cells, sensing (two-component signal transduction systems) and transporter for iron acquisition (K04758, K04759). The forage diet enhanced several genes - encoding for a type IV pilus assembly protein (K02652, K02662 to K02664 and K02666). Finally, type I and II secretion systems (K01993, K02456) were significantly more abundant in samples from forage-fed animals, whilst the gene associated with a type VI secretion system was enriched in concentrate-fed samples (K11907).

Identification of markers of rumen microbial disorder and their link with diet effects on the microbiome

Of the 24 most abundant archaeal and bacterial phyla, 12 phyla including Proteobacteria were more abundant in animals offered the concentrate diet (P < 0.1; Fig. 4 and Additional file 1: Figure S5). In contrast, Euryarchaeota, Planctomycetes, Fibrobacteres and Verrucomicrobia were more abundant in rumen samples from animals offered a forage diet (P < 0.01). Firmicutes, Bacteroidetes and 14 other phyla were not significantly affected by diet. A Proteobacteria ratio above 0.19, suggesting dysbiosis, was only detected in rumen samples from the concentrate diet treatment, and thus, this ratio was on average higher for rumen samples from animals offered the concentrate than the forage diet (P = 0.001; Fig. 5). In addition, this ratio was strongly correlated with diet change (P = 0.001), but not with other factors tested except methane emissions (Additional file 1: Figure S4). In addition, a significant negative correlation was found between the acetate to propionate ratio, a proxy for low ruminal pH (lower ratio) and the Proteobacteria ratio when analysing all samples (Fig. 6). In general, A:P ratio from rumen samples associated with forage grouped together with an average value of 3.77 ± 0.66 (Fig. 6c).

Fig. 4
figure 4

Relative abundance of microbial phyla between diet treatments (n = 50). Grey: samples from forage-fed animals, dark grey: samples from concentrate-fed animals. **P value < 0.01, *P value < 0.05, °P value < 0.1

Full size image
Fig. 5
figure 5

Calculated Proteobacteria ratio over the three experiments (n = 50). Cutoff: values above 0.19 are considered as an indicator of rumen dysbiosis. Grey: samples from forage-fed animals, dark grey: samples from concentrate-fed animals

Full size image
Fig. 6
figure 6

Linear regression for studying the impact of acetate to propionate ratio to Proteobacteria ratio. a All samples (n = 50). b Samples from concentrate-fed animals (n = 16). c Samples from forage-fed animals (n = 34). Equation for the linear regression was included in figure when the difference was significant (P value < 0.05)

Full size image

A high proportion of populations explaining this higher Proteobacteria ratio were composed of families (7/20) and genera (7/20) known to be pathogens in animals, humans or even in plants (Table S4) and were strongly correlated with the Proteobacteria ratio (P < 0.001). For example, Aeromonadaceae including the Aeromonas genus and Enterobacteriaceae were the most abundant Proteobacteria populations known to contain pathogens. Other populations were identified as gut commensals, or with antimicrobial activities, or linked with nutrients (N, S or Fe) or organic matter degradation capacities.

Over the three experiments, 43 genes identified by PLS analysis were considered important in predicting the impact of the Proteobacteria ratio on the microbial community (Additional file 1: Table S5). The relative abundance of the selected genes explained 48% of the factor fitted in the model and 81% of the Proteobacteria ratio. Of these genes, 22 genes were also affected by the dietary treatment—including genes encoding for beta-lactamase (K01467, K07576) and cold shock protein (K03704). For each experiment, a network was generated based on the total KEGG identified genes (n = 4966) including on average 56 genes from the 204 genes and representing about 4.36% of all genes constituting the network. Although these genes were distributed over several clusters, about half of the genes (54%) grouped into one or two dominant clusters when studied per experiment (Additional file 1: Table S5and S6) and still grouped in two dominant clusters when the network analysis combined all experiments (Fig. 7). The most abundant genes (relative abundance above 0.01%) within these two clusters encoded for flagellin (K02406), beta-lactamase (K07576) and type VI secretion system (K11907).

Fig. 7
figure 7

Functional clusters of AMR genes identified using network analysis combining the three independent experiments. Correlation analysis of microbial gene abundance was used to construct networks, where nodes represent microbial genes and edges the correlation in their abundance

Full size image

Discussion

In the present study, the results of three independent experiments were combined. This meta-analysis was used to highlight the importance of diet rather than the host on the structure of rumen microbial genes associated with resistance, colonisation, communication and pathogenicity activities. It is known that diet has a dominant effect on the microbial community composition [40, 41], and here, we first identified that diet influences functional genes associated with AMR and more interestingly microbial genes (e.g. genes encoding for cold shock protein, T6SS, motility, hooking and sensing) allowing the microbial populations to adapt to the new ruminal environment generated by concentrate diet. Information on the composition of the initial rumen microbiome may provide further information, but was not available for this study. However, all animals were offered the same forage-based diet prior to the trial and animals were randomly allocated to diets (balanced for breed) at the start of each experiment. Consequently, there is no reason to believe that the prevalence of antibiotic resistance genes differed at the start of each experiment. Therefore, we are confident in our results that concentrate diet significantly increased the relative abundance and diversity of AMR genes and other genes studied in this manuscript.

The greater abundance of genes encoding for sensing (two-component signal transduction systems) or secretion system (T6SS) in concentrate diet samples confers advantages for microorganisms to sense, respond and adapt to a wide range of environments [42] using different mechanisms [43,44,45]. T6SS which is more prevalent in Proteobacteria helps in colonising the gut [46] and controlling bacterial growth in a disturbed rumen [47]. Moreover, microorganisms carrying genes encoding flagellin, which are enhanced when a high-concentrate diet is fed, are known to develop strategies to evade innate immune system detection [48, 49] and therefore increase the risk of disease. Another host-microbiome interaction system involves fucose sensing, and one gene encoding for alpha-L-fucosidase was significantly enriched in concentrate-fed animals. This gene may enhance the abundance of commensals and also pathogenic bacteria carrying this gene and increase the risk of infection [22]. Finally, it is known that competition between host and pathogens occurs for iron resources. Genes associated with iron storage (ferritin) or transport were differently affected depending of the diet and tend to suggest a lower iron availability for microorganisms including pathogens in concentrate compared to forage-fed animals. It is known that iron serves as a signal for bacterial pathogens to recognise host cells in an iron-depleted environment, potentially leading to disease [50].

Henderson et al. [10] found similar results, demonstrating the predominant influence of diet over the host effect and explaining the differences in microbial community composition. The host effect was identified in the present study as a breed effect, which has been shown in our previous study to be of substantially less impact on the microbiome, than within-breed genetic effects.

In addition, feeding the concentrate diet significantly enhanced the diversity of these rumen microbial genes (higher evenness) including a broader spectrum of mechanisms of resistance compared to the forage diet. The increase in abundance and diversity of AMR and pathogenicity genes in rumen samples from concentrate-fed animals could also be the result of multi-resistant bacteria enrichment [51], or an increase in horizontal gene transfer (HGT) as a result of gut dysbiosis [52, 53] or rumen colonisation [54]. Therefore, the risk of spreading antimicrobial resistance genes between microbial populations belonging to the same or a different phylum and including human pathogens increases [55].

Microbial genes significantly abundant in rumen samples from animal offered the forage diet were mainly associated with activities known to be involved in beneficial host-microbiome interaction and its homeostasis involving non-pathogenic bacteria. For example, antimicrobial peptides like microcin are produced and released by gut epithelial cells and help to maintain the host-microbiome homeostasis [56]. Homeostasis helps to reduce pathogen colonisation [57] without causing collateral damage to host cells [49, 53]. Therefore, it is coherent to find more microcin-resistance genes mostly identified in commensal bacteria [58] and also carrying microcin synthesis genes providing a competitive advantage against enteric pathogens [57] when feeding a high-forage diet, which is known to promote rumen homeostasis [59]. This system is less aggressive and energy demanding than other systems like T6SS used by, e.g. pathogenic Proteobacteria [45]. Forage diets, which contain more fibre, are recognised to enhance gut health in different animal species by modulating the gut microbiome, its impact on the host mucosa and reducing the level of stress or disease compared to grain-fed animals [59, 60].

The results obtained with the concentrate diet can be explained by the necessity for the rumen microbiome to adapt to a new environment [61] and the increase in populations with broader adaptability and colonisation capacities. Interestingly, the relative abundance of Proteobacteria, one phylum containing bacterial populations associated with AMR and pathogenicity [27], was one of the bacterial phyla enriched when feeding the concentrate diet. We also identified bacterial genera within Proteobacteria (e.g. Tolumonas or Methylomonas) and including pathogens (e.g. Aeromonas or Moraxella) with a relative abundance above 0.001% and strongly correlated with the Proteobacteria ratio which may explain the high Proteobacteria ratio detected in 8 rumen samples from animal offered the concentrate diet. Interestingly, the plant pathogen Pantoea, belonging to Proteobacteria, was also significantly more abundant when feeding the concentrate diet and highly correlated with the Proteobacteria ratio, suggesting that microorganisms colonising plants and present in the diet might play a role in the rumen. Functional similarities between microorganisms inhabiting the gut or plant roots and sharing similar ecological niches were found, supporting a potential role of these plant-associated microbial populations during the acidotic challenge [62].

Proteobacteria populations partly explained the change in functional gene composition and diversity observed in samples from animals offered the concentrate diet. A high Proteobacteria ratio was also significantly negatively correlated with a low acetate to propionate ratio (a proxy for low ruminal pH) [12]. It is known that concentrate diets with a high grain content tend to increase propionate concentration and are associated with lower rumen pH, as well as an increase in the abundance of Proteobacteria [12]. The formulation of concentrate diets with respect to the proportion of starch to digestible fibre could be more important than the concentrate type for explaining the effect on rumen pH [63], and this information could be used as a strategy to reduce the detrimental effect of concentrate diet increasing the abundance and diversity of the genes targeted in this study.

We confirmed the relevance of using a Proteobacteria ratio instead of Proteobacteria abundance as a proxy of a microbiome dysbiosis and more pathogenic bacteria may increase the risk of host disease [12, 14]. This ratio was significantly correlated with a gene encoding for a cold shock protein known to have a wider role in bacterial stress tolerance and reinforcing the detrimental effect of the concentrate diet on the rumen microbiome [2].

Finally, the 43 rumen microbial genes identified by PLS analysis to be significantly associated with the Proteobacteria ratio include several AMR genes such as genes encoding for beta-lactamase. Although these genes were distributed over several clusters, most of them were grouped in one major cluster as shown by the network analysis. Therefore, it would be possible to apply the same strategy as Roehe et al. [26] to determine a baseline of these genes for risk assessment.

Conclusions

In conclusion, diets with high fibrous forage components would be recommended over those with high grain components to lower the abundance and diversity of AMR genes and to avoid rumen microbiome dysbiosis associated with pathogenic species among ruminal Proteobacteria. The results presented here will help to improve policies about antimicrobial treatments in beef cattle production and to help avoid AMR gene transmission to bovine-associated human pathogens. We describe AMR gene abundance/diversity in antibiotic-free cattle that complements published information on AMR genes in animals receiving antimicrobials. It will now be important to combine the investigation of AMR usage with dietary regime, to determine whether alterations in diet can lessen the threats associated with AMR in livestock production.