Introduction

Jangkanghwan (JKH) is a Korean traditional food that composed of 12 ingredients, of which some are food ingredients and others are traditional Korean medicine (TKM) ingredients. Among them, radish leaves (Mucheong; Brassica rapa L.), Plantago asiatica L. (Chajeonyeop), Cirsium japonicum var. ussuriense (Regel) Kitam. (Daegye), Saururus chinensis (Lour.) Baill. (Sambaekcho), and Atractylodes macrocephala Koidzumi (Baekchul) are the most abundant ingredients [1]. According to ancient medicinal works and modern Korean medicine studies, all of the above components exert anti-inflammatory [2], anti-bacterial [3], anti-oxidant effects [4], and some also have anti-cancer and anti-tumor effects [5].

It has been previously demonstrated that TKM has a regulatory effect on the mucosal barrier function of the intestine [6]. TKM is usually used clinically by decoction [7]. After entering the intestinal tract, most of its active ingredients are metabolized and transformed with the intestinal flora to change their natural prodrug components, which are then absorbed through the intestines to exert their clinical effects [8]. A few components are directly absorbed in their original form, and others are absorbed by the intestine after they undergo metabolic reactions such as binding and lysis with the intestinal microbiota [9]. Relevant clinical studies have confirmed that TKM has an advantageous effect on promoting the proliferation of probiotics, inhibiting the growth of pathogenic bacteria, and restoring the diversity and improving the balance of the intestinal flora [10].

Traditional herbal medicines consist of plants that are rich in a variety of alkaloids, polysaccharides, glycosides, proteins, amino acids, and other ingredients [11]. The effective ingredients and their metabolites can be used by intestinal probiotics, but not by pathogenic bacteria, thereby selectively promoting probiotic proliferation and inhibiting the growth of pathogenic bacteria [12]. The organic acids in traditional Korean medicines can also acidify the intestinal environment, lower the pH of the intestine, and provide an adapted environment for the growth of probiotics [13].

In addition to normal digestion, absorption, and excretion, the intestine also has an important barrier function [14]. The intestinal mucosal barrier is a relatively complete functional isolation zone of the normal intestine, and it prevents harmful pathogens from entering human tissue cells, organs, and blood circulation through the intestinal mucosa, thereby preventing a series of pathological reactions [15]. There are many bacteria, viruses, and their toxins in the normal human intestinal tract. The intestinal barrier separates the intestinal cavity from the internal environment of the body to maintain a relatively stable environment in the body and ensure the normal life activities of the body [16]. However, under the action of factors such as severe trauma, infection, and stress, the intestinal mucosal barrier can be damaged to varying degrees, which can cause bacteria and endotoxins in the intestine to enter the blood circulation through the intestinal barrier, causing intestinal endotoxemia or sepsis [17].

In recent years, TKM has made great progress in the treatment of impaired intestinal mucosal barrier function, and its advantages have become increasingly prominent. The TKM ingredients in JKH give it the character of a pharmaceutical product, but the food ingredients contained in it also allow JKH to be used as a health food. Therefore, in the current study, we examined the protective effect of JKH as a traditional functional food and medicine on the mouse colonic epithelial barrier and the preventive effect of inflammation through gavage of LPS-induced colonic epithelial barrier damage mice with JKH.

Materials and methods

Introduction of JKH

Experimental JKH was kindly provided by Gahwa Well Food Co., Ltd. (Jincheon, Chungcheongbuk-do, Korea). Information regarding JKH has been previously described. JKH is composed of 12 substances: Mucheong (radish leaves, Brassica rapa L.) (19.2%), Chajeonyeop (Plantago asiatica L.) (12.4%), Daegye (Cirsium japonicum var. ussuriense (Regel) Kitam.) (11.1%), Sambaekcho (Saururus chinensis (Lour.) Baill.) (10.2%), Baekchul (Atractylodes macrocephala Koidzumi) (10.2%), Gokgisaeng (Viscum album var. coloratum) (9.5%), Baekgang (Zingiber officinale Roscoe) (9.5%), Gonpo (Saccharina japonica) (8.7%), Nabok (dried radish, Raphanus sativus L.) (2.6%), Chaga mushroom (Inonotus obliquus (Ach. ex Pers.) Pilát) (2.6%), Nalgye (Cinnamomum cassia Blume) (2.6%), and Jukyeom (bamboo salt) (1.4%) [1].

Animal experimental design

Forty 7-week-old BALB/C mice weighing 20.0 ± 2.0 g were randomly divided into four groups after 1 week of adaptive feeding, with 10 mice in each group. All mice had free access to AIN-93G diet food and tap water during the 17-day experiment. The normal group and LPS group mice were gavaged with tap water every day, and the JKH Low-dose (JKHL) group (700 mg/kg BW) and JKH High-group (JKHH) group (2100 mg/kg BW) [1] mice were gavaged daily according to the concentration of JKH solution. On days 15, the mice in the normal group were intraperitoneal injected with physiological saline, and the remaining 30 mice were intraperitoneal injected with 30 mg/kg BW LPS solution. The food intake of the mice was weighed daily, and feces were collected [18].

After all experiments were completed, CO2 treatment was used to euthanize the mice, and then, orbital venous blood collection was performed. The liver, kidney, and intestinal lymph nodes were removed while the mouse was on a sterile operating table, and then, the organs were weighed. The colon was completely removed, its length was measured, it was photographically recorded, and then, it was stored at − 80 °C for later use (Fig. 1). The animal study was reviewed and approved by the protocol for these experiments was approved by the Ethics Committee of Chongqing Collaborative Innovation Center for Functional Food (202101005B), Chongqing, China. The experimental process was in accordance with 2010/63/EU directive.

Fig. 1
figure 1

Animal experimental design of LPS-induced colonic epithelial injury in BALB/C mice

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Diarrhea after LPS administration

The mouse feces collected daily were weighed and recorded, dried in an oven at 55 °C for 48 h, placed in a drying dish to equilibrate to room temperature, and the dried feces were then weighed. The above operation was repeated until the stool weight difference was less than 1%, and then, the dry weight of the stool was recorded.

$${\text{Fecal}}\,{\text{moisture}}\,{\text{content}}\,(\% ) = {{{\text{Dry}}\,{\text{weight}}\,{\text{of}}\,{\text{feces}}\,({\text{g}})} \mathord{\left/ {\vphantom {{{\text{Dry}}\,{\text{weight}}\,{\text{of}}\,{\text{feces}}\,({\text{g}})} {{\text{Wet}}\,{\text{weight}}\,{\text{of}}\,{\text{feces}}\,({\text{g}}) \times 100}}} \right. \kern-\nulldelimiterspace} {{\text{Wet}}\,{\text{weight}}\,{\text{of}}\,{\text{feces}}\,({\text{g}}) \times 100}}.$$

Bacterial translocation assay

The liver, kidney, and mesenteric lymph nodes (MLN) were weighed and then homogenized. Each of the tissue homogenates was spread (0.1 mL) on plate count agar (PCA). After anaerobic incubation at 37 °C for 24–48 h, the number of colonies was counted according to the colony dilution factor, subsequently converted to the amount of bacteria per gram of tissue, and expressed in colony-forming units (CFUs). Greater than 100 CFU/g was recorded as a positive plate, and less than 100 CFU/g was recorded as a negative plate [19].

Determination of the intestinal mucosa permeability

Food and water were withheld from the mice for 4 h before they were killed. In the meantime, fluorescein isothiocyanate-dextran (FITC-D, MW 4000) was used to intragastrically (60 mg FITC-D/100 g) the mice. After 4 h, the serum was collected, and the fluorescence density of each sample was measured with a microplate reader at an excitation wavelength of 485 nm and the emission wavelength of 528 nm to determine the concentration of FITC-D in the serum [20].

Serum inflammatory cytokine test

The collected mouse blood was centrifuged at 3000×g and 4 °C for 10 min, and then, the serum was collected. According to the ELISA kit instructions, the levels of IL-1β, IL-6, TNF-α, and IFN-γ in mouse serum were measured.

H&E staining of colon tissue

Colon tissues were prepared for histopathological analysis after fixation in neutral buffered formalin (buffered 10% formaldehyde, pH 7.2). Paraffin sections of 5-μm thickness were processed for hematoxylin and eosin (H&E) staining. The slices were observed, and photographs were obtained via optical microscope (BX43, OLYMPUS, Tokyo, Japan).

qPCR

For qRT-PCR, 100 mg colon tissue was homogenized with 1 mL TRIzol reagent (Invitrogen, Carlsbad, CA, USA) to extract RNA from the tissue, and the RNA concentration was adjusted to 1 μg/μL. A cDNA reverse transcription kit (Thermo Fisher Scientific, USA) was used to reverse transcribe the RNA to cDNA. The synthesized cDNA was then mixed with 10  µL SYBR Green PCR Master Mix (Thermo Fisher Scientific, USA), 2  µL primers (Table 1), and distilled water. Quantitative PCR was performed in an automatic qPCR thermocycler at 95 °C for 60 s; 40 cycles at 95 °C for 15 s, 55 °C for 30 s, and 72 °C for 35 s; and a final step of 95 °C for 30 s and 55 °C for 35 s. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the internal reference gene, and the 2−ΔΔCt formula was used to calculate the relative mRNA transcription level.

Table 1 Primer sequences of colon tight junctions (TJs) in BALB/C mice that underwent LPS-induced colonic epithelial injury
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Western blot

For the Western blot, 100 mg of colon tissue was homogenized with 1 mL radioimmunoprecipitation assay (RIPA, Thermo Fisher Scientific, USA) and 10 μL phenylmethylsulfonyl fluoride (PMSF, Thermo Fisher Scientific, USA), and then centrifuged at 12,000×g for 5 min at 4 °C. The protein was quantified using a bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific, USA). The protein samples were mixed with sample buffer (Thermo Fisher) 4:1, heated at 95 °C for 5 min, and then, the samples were loaded into the wells of a sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gel, and the gel was electrophoresed at 100 V. The bands on the SDS-PAGE gel were transferred to a polyvinylidene difluoride (PVDF) membrane. Then, the PVDF membrane was blocked with 5% skimmed milk for 1 h and incubated with primary antibody (JAM-1: BS-3651R; ZO-1: 40-2200; Claudin-1: 71-7800; Occludin: 40-4700; Cingulin: 651122; beta-actin: MA5-11869, Thermo Fisher Scientific, USA) overnight at 4 °C. The membrane was washed, secondary antibody (Thermo Fisher Scientific, USA) was added, and the blot was incubated for 1 h. Western enhanced chemiluminescence (ECL) substrate (Thermo Fisher Scientific, USA) was used to perform chemiluminescence visualization, and images were obtained using iBright (Thermo Fisher Scientific, USA) for subsequent analysis.

Statistical analysis

Three measurements of serum and tissue samples were performed in parallel, and the average value was calculated. SPSS software (SPSS v.25 for Windows, IBM Software Group, Chicago, IL, USA) was used to average and analyze the data. The Duncan multiple range test was used to evaluate the difference between the mean values for each group by one-way analysis of variance (ANOVA). Differences with p < 0.05 were considered statistically significant.

Results

Food intake analysis

The administration of LPS by injection can cause pain in mice, which leads to decreased appetite and food intake. During the 17-day experiment, the dietary intake of all mice in the first 15 days was nearly the same, 5.0 ± 0.16 g. However, after the LPS injections were initiated on the 15th day, all mice except those in the normal group showed a decrease in food intake that continued until the end of the experiment. Among them, the decrease in food intake of the LPS group mice was the greatest, with consumption measured at 3.7 ± 0.15 g on the 17th day. The food intake of the JKHH group and JKHL group mice on the 17th day was 4.7 ± 0.21 g and 4.0 ± 0.11 g, respectively. It can be clearly seen that JKH can alleviate the decreased food intake in mice (Fig. 2a).

Fig. 2
figure 2

Daily food intake (a) and fecal moisture content (b) of BALB/C mice that underwent LPS-induced colonic epithelial injury. a−dMean values with different letters in the different polyline are significantly different (p < 0.05) according to Duncan’s multiple range test. LPS: 30 mg/kg BW injection on 15 days; JKHL, low dose of JKH (700 mg/kg BW) + LPS 30 mg/kg BW injection on 15 days; JKHH, high dose of JKH (2100 mg/kg BW) + LPS 30 mg/kg BW injection on 15 days

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Organ index analysis

The weight of mice and organ weights indicate that LPS causes weight loss in mice, but it will cause the liver, kidneys, and MLN to increase in weight. Therefore, the liver, kidney, and MLN indexes for all LPS-induced mice all increased by different degrees compared with the normal group mice. Among them, the greatest increase was for the LPS group mice, and they exhibited an organ index that was approximately twice that of the normal group. JKH relieved the edema of organs in mice, with a stronger effect at higher concentrations (Table 2).

Table 2 Organ indexes of BALB/C mice that underwent LPS-induced colonic epithelial injury
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Diarrhea after LPS administration

The water content of mouse feces was measured to express the severity of diarrhea caused by LPS in mice. Through experimentation (Fig. 2b), in the first 15 days, the average fecal water content of mice was 56.19 ± 0.53 g. After the injection of LPS on the 15th day, the mouse feces became soft and thinner on the 16th day. The water content of feces significantly increased on day 16–17, especially on day 17. The fecal water content of mice in the LPS-induced group increased to 121.23% of the average fecal water content of the previous 15 days. The fecal water content of the JKHL and JKHH group mice was lower than that of the LPS-induced group, which was 115.30% and 107.37% of the average fecal water content during the previous 15 days, respectively.

Serum inflammatory cytokine analysis

IL-1β, IL-6, TNF-α, and IFN-γ are four typical pro-inflammatory cytokines, and the serum content of these four cytokines can indicate the degree of inflammation in the mouse body. The expression of pro-inflammatory cytokines in the four groups in Fig. 3 shows that the inflammation level of the normal group is the lowest of the four groups, and the inflammation level in the LPS-induced group is the highest of the four groups, with a value that is at least 1.7 times higher than the normal group, and the highest is six times greater. Gavage of JKH can alleviate the body's inflammatory response caused by LPS. Compared with the normal group, the inflammation level of the JKHH group was generally decreased to no more than twice that of the normal group.

Fig. 3
figure 3

Cytokine indexes in the serum of BALB/C mice that underwent LPS-induced colonic epithelial injury. a−dMean values with different letters in the different bar are significantly different (p < 0.05) according to Duncan’s multiple range test. LPS: 30 mg/kg BW injection on 15 days; JKHL, low dose of JKH (700 mg/kg BW) + LPS 30 mg/kg BW injection on 15 days; JKHH, high dose of JKH (2100 mg/kg BW) + LPS 30 mg/kg BW injection on 15 days

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Colon bacteria translocation analysis

When the intestinal mucosal barrier is damaged, the microorganisms and toxins in the intestine can break through the intestinal mucosal barrier and enter the portal vein and lymphatic system. Thereby, this will cause bacterial translocation and can even develop into systemic inflammatory response syndrome (SIRS) or multiple organ failure syndrome (MODS). Measuring the bacterial translocation in mice can reflect the degree of intestinal mucosal permeability. No bacterial translocation was detected in the liver, kidney, or MLN of mice in the normal group, but the positive rate of bacterial translocation in the liver, kidney, and MLN of mice in the LPS group exceeded 86%. The intestinal permeability in JKH intervention mice was significantly decreased, and the positive rate of bacterial translocation in the liver, kidney, and MLN of the JKHH group mice was only 20% (Table 3).

Table 3 Bacterial translocation in the liver, spleen, and mesenteric lymph node
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Colonic permeability analysis

The permeability of the intestinal mucosa in the normal group to fluorescent macromolecules was very small, at only 4.14 ± 0.51 μg/mL. The injection of LPS significantly increased the permeability of the intestinal mucosa to molecular substances. The concentration of fluorescent substances detected in the serum of the LPS-induced group mice was 69.88 ± 1.79 μg/mL, which was approximately 16.8 times that of the normal group mice. The concentration of fluorescent substances in the serum of the JKHL and JKHH group mice was 7.8 and 4.3 times that of the normal group, respectively, which was significantly lower than that of the LPS-induced group (Fig. 4).

Fig. 4
figure 4

Colon FITC-D permeability in BALB/C mice that underwent LPS-induced colonic epithelial injury. a−dMean values with different letters in the different bar are significantly different (p < 0.05) according to Duncan’s multiple range test. LPS: 30 mg/kg BW injection on 15 days; JKHL, low dose of JKH (700 mg/kg BW) + LPS 30 mg/kg BW injection on 15 days; JKHH, high dose of JKH (2100 mg/kg BW) + LPS 30 mg/kg BW injection on 15 days

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Colon histopathological observation

In the normal group, the colon structure was clear, the mucosal epithelial cells were neatly arranged, the intestinal glands were regular, and the goblet cells were abundant. In the LPS group, the colonic mucosa of the mice was shedding and necrotic, forming ulcers of varying degrees, a large number of inflammatory cells infiltrated, mucosal glands were damaged, and the arrangement was sparse and disordered, with varying degrees of expansion. There was no obvious ulcer formation in the colonic mucosa of mice in the JKH group, and the mucosal edema and congestion and inflammatory cell infiltration were reduced. There was less damage to the high-dose and lower-dose groups (Fig. 5).

Fig. 5
figure 5

Pathological observation by H&E staining of colon tissue from BALB/C mice that were subject to LPS-induced colonic epithelial injury. The arrow in the picture indicates the inflammatory and damaged parts of the colon. LPS: 30 mg/kg BW injection on 15 days; JKHL, low dose of JKH (700 mg/kg BW) + LPS 30 mg/kg BW injection on 15 days; JKHH, high dose of JKH (2100 mg/kg BW) + LPS 30 mg/kg BW injection on 15 days

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mRNA and protein expression analysis of colon tissue

In this experiment, the mRNA and protein expression of the five most important genes in tight junctions (TJs) were measured. The levels of mRNA and protein expressed by the five genes in the colon of the normal group of mice were the highest among the four groups of mice. The mRNA and protein expression of the five genes in the colon of mice from the LPS group was the lowest. JKH can alleviate the damage to TJs caused by LPS, and enhance the expression of mRNA and the proteins produced by the expression of the JAM-1, ZO-1, claudin-1, occludin, and cingulin genes (Fig. 6).

Fig. 6
figure 6

TJ mRNA (a) and protein (b) expression in the colon tissue of BALB/C mice that underwent LPS-induced colonic epithelial injury. a−dMean values with different letters in the different bar are significantly different (p < 0.05) according to Duncan’s multiple range test. LPS: 30 mg/kg BW injection on 15 days; JKHL, low dose of JKH (700 mg/kg BW) + LPS 30 mg/kg BW injection on 15 days; JKHH, high dose of JKH (2100 mg/kg BW) + LPS 30 mg/kg BW injection on 15 days

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Discussion

JKH contains numerous herbal and food ingredients, among which radish leaves, ginger, and bamboo salt are food ingredients; plantain, Asian lizard’s tail, white atractylodes, mistletoe, and cinnamon are considered as traditional Korean medicine ingredients; and Korean milk thistle, ginger, royal kombu, and Chaga mushroom are considered both medicine and food homology ingredients. In previous analysis and research, it was found that JKH contains a large amount of active ingredients with anti-inflammatory effects that also have a certain preventive effect on ulcerative colitis [1].

The intestine is not only an important organ for digestion and absorption, but it is also the largest immune organ in the body. It plays an important role in the body's non-specific anti-infection defense system [21]. The intestinal mucosa can monitor and remove intestinal bacteria, toxins, potentially harmful biological peptides, and food antigens to ensure the integrity of the body [22]. A complete intestinal mucosal barrier is the basis for maintaining the multiple functions of the intestine [23].

The intestinal mucosal barrier is mainly composed of four parts: the biological barrier, chemical barrier, mechanical barrier, and immune barrier [24]. The biological barrier consists of the normal intestinal flora, such as Lactobacillus and Bifidobacterium [25]; the chemical barrier is mainly composed of mucus secreted by epithelial cells, as well as intestinal symbiotic bacteria, bile, lysozyme, probiotics, and substances secreted by various digestive enzymes [26]; the mechanical barrier is composed of TJs of intestinal epithelial cells and intestinal mucus [27]; and the immune barrier is mainly composed of secretory immunoglobulins, related lymphoid tissues of the digestive tract, and defensins [28]. These four barriers constitute a huge and complex three-dimensional protective system for the intestines, in which the synergy between the mechanical barrier and the immune barrier is the core of maintaining the homeostasis of the intestinal environment [29]. Therefore, the protection of the intestinal mucosal barrier function is very important, and the use of natural food and drug ingredients for intestinal mucosal protection has become a heavily researched area.

As the main component of the cell wall of gram-negative bacteria, LPS itself has no toxic effects, but when LPS enters the body’s circulatory system, interaction with host cells occurs that results in stimulation of immune cells and subsequent synthesis and release of inflammatory factors, thereby exhibiting biological activity [30,31,32]. LPS usually acts on systemic inflammation, but may also cause gastrointestinal mucosal injury, the intestine is one of the target organs of LPS [33,34,35]. LPS can directly trigger the inflammatory response of intestinal epithelial cells, destroy the integrity of the intestinal mucosal barrier, and cause mucosal barrier dysfunction [36,37,38]. It is clear from our experimental data that the intervention of JKH increases the food intake of mice, reduces the severity of mouse diarrhea, reduces the level of inflammatory factors in the serum of mice, increases the permeability of the intestinal mucosa, prevents bacteria and other macromolecular substances from entering the lumen circulation, and maintains the integrity of intestinal epithelial cells.

In the intestinal mucosal barrier, the most important barrier is the mechanical barrier, which is composed of intact intestinal epithelial cells and TJs between adjacent intestinal epithelial cells [39]. The TJ is a protein complex composed of a group of transmembrane proteins (including occludin and claudin), zonula occludens (ZO) proteins, and junction adhesion molecules (JAM) [40]. It is a barrier that selectively allows the diffusion of molecules and ions between cells [41]. Its opening and closing are regulated by various physiological and pathological stimuli such as intestinal lumen materials and cytokines, and it plays an important role in maintaining normal intestinal mucosal epithelial permeability [42]. It allows the required dietary nutrients, electrolytes, and water to enter the cavity to circulate, and prevents the entry of foreign antigens, microorganisms, and their toxins [43]. If the intestinal mucosal barrier is damaged, it will cause the destruction of the TJ between epithelial cells and increase the permeability [44]. Due to the increased permeability, the intestinal mucosa is more susceptible to constant stimulation by pathogens, bacterial toxins, microorganisms, and other factors, thereby promoting the recruitment of neutrophils, leading to the release of excessive inflammatory mediators and causing further damage to the intestinal tissues [45, 46].

Studies have confirmed that the claudin and occludin proteins are important components of TJs [47]. Under normal conditions, occludin is located on the top of the sidewall of epithelial cells, separating the top and basal sides of the cells, and preventing the material in the epithelial intercellular space from overflowing and macromolecules from passing through the intercellular space [48]. The claudin protein is considered to be the backbone of TJs and plays an important role in the ability of TJs to seal cell spaces [49]. The JAM-1 protein is located at the top of the intercellular space and plays an important role in the inflammation process [50]. The expression of JAM protein is usually related to the recruitment of TJ components, and it also supports the TJ assembly [51]. ZO-1 is also one of the important components of TJs. It is involved in regulating cell material transport and maintaining epithelial polarity, and is also related to information transmission and regulation of processes such as cell proliferation and differentiation, tumor cell metastasis, and gene transcription [52]. Cingulin binds to bundle actin filaments and forms a bundle that interacts with myosin II and several TJ proteins, including ZO-1, ZO-2, ZO-3, and occludin [53]. In addition, cingulin forms a complex with the TJ membrane protein JAM-1 that acts on TJs together [54].

In patients with active inflammatory bowel disease with impaired intestinal epithelial barrier function, the expression of mRNA and TJ proteins, such as occludin, claudin-1, ZO-1, cingulin, and JAM-1, was significantly reduced in the lamina propria of the colonic mucosa. The mRNA and protein expression of occludin, claudin-1, ZO-1, and JAM-1, which are important components of TJs, significantly increased in the colon tissue of mice treated with JKH. This indicates that JKH can maintain the composition of the TJ and the shape of the intestinal epithelial barrier, so as to protect the intestinal mucosal barrier.

Our experiments have confirmed that the traditional Korean medicinal food JKH alleviates the functional damage of mouse colonic epithelial cells caused by LPS, effectively reduces the inflammation in the mouse intestine, and also protects the normal function and state of the mouse colon. It also can prevent bacterial displacement caused by colon damage. Additionally, JKH alleviated the weight loss and decreased food intake, and also protected the TJ tissues of the mouse colon and the tissues of other body organs. In future research, we will further determine the effective ingredients in JKH and the mechanism used by each ingredient to protect the colon. Our data indicate that there is great potential for the use of JKH as a supplementary food in the treatment of patients with colon injury so that they can quickly restore their health