Background

Vector mosquitoes inflict more human suffering than any other organism and spread diseases that kill more than one million people each year. Mosquito-borne illnesses, among the most complex infectious diseases to prevent and control, have resurged worldwide and pose threats for epidemic outbreaks in developed countries, including the United States. Given poor progress in vaccine development and distribution, mosquito control is the primary mechanism for disease control. However, the emergence of insecticide resistance and a lack of support for mosquito control programs compromise strategies for managing mosquitoes, resulting in a need for the development of new approaches to combat these insect vectors of human disease [1]. The mosquito genome projects have facilitated research in many new facets of mosquito biology [2–5]. However, despite substantial progress in mosquito genomic and genetic research, very few cis-regulatory elements (CREs), DNA sequences that control gene expression, have been identified in the mosquito genomes. CRE discovery is challenging because these elements are typically short sequences contained within vast stretches of intergenic DNA [6]. Although computational approaches resulted in the identification of a number of mosquito CREs [6], CRE discovery has not continued to progress, and regulatory regions for most mosquito genes remain unknown. This deficiency—a significant gap in our basic knowledge of mosquito genetics—has resulted in a lack of drivers to manipulate gene expression in selected tissues at specific times, the inability to properly dissect gene regulatory networks in mosquitoes, and difficulty in understanding the biological meaning of genetic variation that resides in non-coding regions.

FAIRE-seq, formaldehyde-assisted isolation of regulatory elements paired with DNA sequencing [7], is emerging as a powerful new approach for global biochemical isolation of CREs through their lack of association with nucleosome proteins. FAIRE, which exploits the ability of formaldehyde exposure to form crosslinks between interacting nucleosomal histones and DNA, has emerged as a powerful new approach for genome-wide identification of regulatory elements [7–9]. During the FAIRE process, chromatin is cross-linked with formaldehyde, sheared, and then phenol-chloroform extracted, permitting recovery of open chromatin DNA fragments that are not bound by nucleosomes, an evolutionarily conserved indicator of regulatory activity [10]. FAIRE has many advantages over alternative methods, one being that the recent pairing of this technique with next-generation sequencing, FAIRE-seq, permits straightforward and genome-wide high throughput detection of CREs [7]. FAIRE-seq is technically straightforward and involves fewer steps, reagents, and variables than alternative methodologies. Use of FAIRE eliminates the tedious titration of DNAse activity and limits concerns about variations in nuclei preparations that affect other protocols. Unlike chromatin immunoprecipitation, there is no dependence on antibody supply/quality or the production of tagged proteins. Also, unlike DNAse sensitivity assays, FAIRE requires no cellular treatments prior to crosslinking and better captures the endogenous chromatin state [8].

Here, we describe a FAIRE-seq investigation in the human disease vector mosquito Aedes aegypti. A. aegypti, a container-breeding mosquito that is closely associated with humans and their urban dwellings, transmits viruses that cause Zika, yellow fever, chikungunya, and dengue, the most widespread and significant arboviral disease in the world. A third of the world’s population is at risk for dengue virus (DENV) infection, a leading cause of illness and death in the tropics and subtropics. DENV is transmitted to as many as 400 million people each year when they are bitten by infected Aedes mosquitoes [1]. A. aegypti strains that are susceptible (Moyo-S) and refractory (Moyo-R) to DENV infection have been selected from a common genetic background [11, 12]. Genome-wide transcriptome comparisons suggest that modular expression of genes is significantly different in the two strains and that many genes (~2500) may contribute to susceptible/refractory responses of A. aegypti to DENV infection [13]. In a companion study, next generation sequencing identified >13,000 single nucleotide polymorphisms (SNPs) in the two strains [14–16]. In this investigation, FAIRE-seq analysis of open chromatin in A. aegypti permitted genome-wide discovery of cis-regulatory elements, which facilitated analysis of genetic variation in non-coding cis-regulatory elements that may contribute to mosquito susceptibility and responses to DENV infection.

Methods

Ethics

This study did not include human subjects, human data, or vertebrate animals.

Mosquito rearing and egg collection

The A. aegypti Liverpool-IB12 (LVP-IB12) strain, from which the genome sequence [3] was derived, was used in this investigation. Mosquitoes were maintained in an insectary at 26 °C, ~80 % humidity, under a 12 h light and 12 h dark cycle with 1 h crepuscular periods at the beginning and end of each light cycle. Larvae fed on a suspension of dried beef liver powder, and adults were provided cotton soaked with 10 % sugar solution. For blood feeding adult females, an artificial membrane feeding system was used in conjunction with sheep blood purchased from Hemo-Stat Laboratories (Dixon, CA). Females deposited eggs on paper toweling during two-hour eggs collections. Eggs were maintained in the insectary for an additional 49 h.

FAIRE

DNA was prepared using a modified version of the Drosophila melanogaster embryonic tissue protocol [17], which is based on Simon et al. [7] methodology. DNA processed in this manner will hereafter be referred to as FAIRE DNA. In summary, 100 mg of eggs were treated with 50 % bleach for 3 min. and then rinsed thoroughly with distilled water. Crosslinking was performed for 15 min using 10 mL 0.4 % formaldehyde in PEM buffer (100 mM PIPES disodium salt, 2 mM EGTA, 1 mM magnesium sulfate; pH 7) in a 60 °C water bath. Following crosslinking, which was quenched through addition of glycine, embryonic nuclei were pelleted through centrifugation at 1500 RCF at 4 °C for 2 min. The nuclei were resuspended in Buffer A (10 mM HEPES pH 8, 10 mM EDTA, 0.5 mM EGTA, 0.25 % Triton X-100) and homogenized with a pestle. The lysate was passed through Miracloth to remove debris. The nuclei were pelleted again, resuspended in FAIRE lysis buffer (2 % Triton X-100, 1 % SDS, 100 mM NaCl, 10 mM Tris-Cl pH 8, 1 mM EDTA), and subjected to six rounds of bead beating with 0.5 mm glass beads/vortexing at 4 °C. Chromatin was sonicated using a Branson 250 ultrasonifier outfitted with a microtip (6 × 30 s with 1 s pulse, 0.5 s stop, 22 % amplitude), generating a size range of 300–500 bp fragments. Soluble chromatin was then recovered following centrifugation at 15,000 G for 5 min at 4 °C. FAIRE DNA was recovered from the cell lysate through phenol chloroform extraction in which open-chromatin DNA was recovered from the aqueous phase. The recovered open chromatin was treated with RNAse A and proteinase K, and then purified through a Qiaquick spin-column (Qiagen, Valencia, CA). The yield of FAIRE DNA (~1 μg) was sufficient for sequencing and corresponded to ~1 % of total DNA, well within the acceptable yield recommended by Simon et al. [7].

Illumina library construction and sequencing

FAIRE DNA Illumina libraries were prepared by the University of Notre Dame Genomics and Bioinformatics Core facility using the TruSeq kit (Illumina, San Diego, CA) per the manufacturer’s guidelines. 1 μg of FAIRE-enriched DNA was processed following the Illumina TruSeq DNA LT sample preparation protocol. Briefly, samples were end repaired and 3’ adenylated. Illumina adapters were ligated to the template, then purified and size-selected (for 100–300 bp fragments) on an agarose gel. A 10-cycle PCR reaction enriched for ligation products containing both Illumina adapters. Sample concentration was measured on a Qubit fluorometer (Life Technologies, Grand Island, NY), and sample size distribution was assayed on an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA). Next-generation Illumina Sequencing (HiSeq 50 bp paired-end sequencing) with an Illumina HiSeq 2000 (BGI Americas Corporation, Cambridge, MA) was used to generate ~150 million reads for each of three replicate FAIRE DNA preparations.

Analysis of FAIRE sequencing data

Raw sequences were trimmed of adapters with Trimmomatic [18] version 0.32 and assessed for quality with FastQC [19] version v0.10.1. Trimmed sequences were aligned to version three of the A. aegypti scaffolds reference genome [20, 21] using the backtrack algorithm in BWA [22] version 0.5.9-r16 for three replicates. The third replicate, however, required further processing due to an unknown segmentation fault. A subset of fifty thousand reads containing one or more reads that could not be processed by BWA was located using Makeflow_BWA [23] and removed to enable successful alignment. Reads were further filtered with Samtools [22] before checking for reproducibility of replicates with the Irreproducible Discovery Rate (IDR) framework as described [24, 25] and calling peaks with MACS2 [26]. These peaks are hereafter referred to as FAIRE peaks (FPs). The maximum insert size itself was estimated empirically using BWA and resulted in calling peaks with an extent size of 550 bp.

Prediction of known transcription factor binding sites

Overrepresentation analysis of FPs for known transcription factor binding sites (JASPAR eukaryote, data downloaded in March 2014) was performed. The ‘Clover’ (Cis-eLement OVERrepresentation) tool [27] was used to determine motif incidences in the FPs. In the first step, the degree of motif [transcription factor (TF)–binding] incidences in the FPs was expressed by a score that was calculated. In the second step, the p‐value was obtained from the raw score, which is essentially the probability that the motif incidence score is either equal or greater to that of the threshold (user defined, set to a default value of 6) by chance. The background sequences were generated for Clover analysis as described previously [28].

Association of FPs with gene expression data in embryos

To determine if FP sequences identified in this study are associated with immediate (<1 kb) 5’ upstream regulatory regions of genes expressed during this embryonic stage, FPs were compared with available 48–52 h embryonic transcriptome data previously described by Akbari et al. [29].

Identification of SNPs in FPs

Single nucleotide polymorphisms (SNPs) within FP sequences were determined from a set of SNP data generated from a companion study (which will be described in its entirety elsewhere) in which Illumina sequencing of genomic DNA from two A. aegypti lab strains, Moyo-S and Moyo-R, was performed [14–16]. Both strains have been described previously [11, 12]. The SNP positions predicted within each supercontig were used to assess if they are present within the FP start and end coordinates within the same supercontigs.

Analysis of genomic distribution of FPs

The map locations of FPs were compared relative to the genomic coordinates of gene models (both protein coding and non-coding genes) to determine their associations. All 3’ and 5’ untranslated region (UTR) sequences of A. aegypti genes were obtained from VectorBase (VB) [20, 21], and the UTR regulatory elements present in those sequences were identified by using UTRScan [30].

Transgenic reporter generation and analysis

FP DNA sequences of interest were PCR-amplified from A. aegypti genomic DNA and cloned into plasmid pattBnucGFPs, a ϕC31-enabled Drosophila transformation vector containing enhanced green fluorescent protein (EGFP) under the control of a minimal hsp70 promoter (graciously provided by M.S. Halfon). Transgenic Drosophila were produced at Rainbow Transgenic Flies, Inc. (Camarillo, CA) by injection into line PBac{y[+]-attP-9A}VK00027 (Bloomington stock 9744 [31]). Tissue from transgenic animals was collected and fixed as described previously [32]. Tissues were mounted and imaged on a Zeiss 710 confocal microscope using Zen software or a Zeiss Axioimager equipped with a Spot Flex camera and Spot Digital Imaging software. Images were processed with Adobe Photoshop software.

Results and discussion

Gene-centric distribution of FPs

FAIRE-seq open chromatin profiling was performed to identify regulatory DNA in the A. aegypti genome. For these studies, embryos were collected 50 +/− 1 h after eggs were laid, a time point that coincides with the onset of axon pathfinding [33], a topic of interest to our laboratory [33–35], and a time period for which transcriptome data are available [29]. The FAIRE procedure was optimized using these A. aegypti embryos as input tissue until a 1 % FAIRE DNA yield (with respect to the total input DNA) was obtained, an amount that is within the optimal range noted by Simon et al. [7]. FAIRE-seq replicate experiments were highly reproducible, as evidenced by IDR [24, 25] analysis (Additional file 1) which detected no significant differences between three replicate experiments, the data from which were subsequently combined for downstream analyses. FAIRE-seq reads [36] were mapped to version three of the A. aegypti scaffolds reference v.4 [20, 21] and have been made accessible through the VB Genome Browser (Fig. 1). In total, FAIRE-seq open chromatin profiling resulted in the identification of ~121,600 FPs (Additional file 2) [36], a number which is consistent with results reported in other systems [7].

Fig. 1
figure 1

Visualization of FAIRE-seq data in the VB genome browser. FAIRE-seq reads [36] mapped to version three of the A. aegypti scaffolds reference v.4 [20, 21] are accessible through the VB Genome Browser. FPs in the supercontig 1.551: 476,900–630,100 bp region are shown. The peak marked with an asterisk corresponds to reporter A in Table 2. The gene accession numbers noted here and in all subsequent tables and supplementary materials correspond to VB [20, 21]

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FAIRE-seq identifies regulatory elements in the A. aegypti genome

Several lines of evidence indicate that FAIRE reliably identified regulatory elements in A. aegypti. In total, 5 % of the FPs were found to be located at proximal promoters (Additional file 3), a figure that is consistent with reports in other systems [37, 38]. These sequences can include both core promoters as well as regulatory elements adjacent to the promoters. However, one limitation of FAIRE is that other assays, including DNAse-seq, may be better for identification of nucleosome-depleted promoters of highly expressed genes [7, 38]. The aggregated FAIRE signal around all transcription start sites (TSSs) showed higher frequency within 100–200 bp upstream of TSSs (Fig. 2), which is also expected [7]. Furthermore, transcription factor binding sites are often associated with regulatory sequences in eukaryotes [39]. Known transcription factor binding sites are enriched in the FPs (Table 1) with respect to the rest of the genome. Among them, the top 20 TF-binding sites that are significantly enriched in FPs are shown in Fig. 3. Of these, the following consensus sequences are most abundant: FoxA1, Gfi, Hunchback, Klf4, MYB/ph3 and Sox9. Some of these elements, particularly Hunchback and FoxA1, were also abundant in FPs localized to the UTRs and intragenic regions (these regions are discussed further below).

Fig. 2
figure 2

FPs are enriched around TSSs. The aggregated FAIRE signal adjacent to transcription start sites (TSSs) was increased within 100–200 bp upstream of TSSs

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Table 1 Consensus binding sites enriched in FPs
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Fig. 3
figure 3

Top TF-binding sites significantly enriched in FPs. The top 20 TF-binding sites that are significantly enriched in the FPs are shown

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Next, the proximity of FPs with respect to genes known to be expressed in A. aegypti embryos was examined. The developmental transcriptome of A. aegypti was recently described [29]. RNA-seq data is available for 48–52 h embryos [29], which includes the time span analyzed in the FAIRE-seq study (49–51 h). Analysis of the FAIRE data with respect to RNA-seq data revealed that 78 % (4733) of genes expressed at this developmental stage have a 5’ flanking (within 1 kb upstream of the TSS) or intragenic FP. In the majority of cases (89 %), these FPs are located within 1 kb upstream of the TSS of the gene, and in the other 11 % of cases, they reside within the genes in question. For cases in which FPs localized to within 100 bp upstream of the TSS, the gene has a relatively higher expression level (1.4 fold) compared to genes for which FPs were localized to more distant locations. This suggests that positional proximity of regulatory sequences may influence expression of the downstream genes, at least during embryonic development in A. aegypti.

To further assess whether FAIRE-seq identified regulatory regions in the A. aegypti genome, a number of FP DNA sequences were tested for their ability to drive gene expression in vivo. Although transgenic technology is available in A. aegypti, transgenic generation in D. melanogaster is mature, quick, and inexpensive, and it is relatively easier and cheaper to maintain fruit fly stocks. Given the merits of Drosophila transgenics, 11 A. aegypti FP DNA sequences (Table 2) were cloned into a Drosophila transformation vector containing EGFP under the control of a minimal hsp70 promoter. 100 % of these elements were confirmed to drive EGFP reporter expression in D. melanogaster (Fig. 4). Although the FAIRE-seq study was performed in A. aegypti embryos, in addition to embryos (Fig. 4a, b), a number of the FP sequences identified were found to be capable of driving gene expression at later stages of the Drosophila life cycle, including the larval (Fig. 4c, e, f, i, j, k), pupal (Fig. 4d), and adult (Fig. 4g, h) stages. These findings suggest that some of the regulatory sequences that function in embryos are also active at later stages of the life cycle, including adults. However, a recent FAIRE-seq study in D. melanogaster showed that following cell-type specification in the appendages, open chromatin profiles changed as the appendages progressed toward terminal differentiation, suggesting that stage-specific functions also require opening of new regulatory sites or the closing of existing sites [17]. In the future, it will be interesting to generate A. aegypti transgenic reporters to examine the activity of the regulatory elements assessed here in Drosophila (Table 2, Fig. 4) at multiple stages of the mosquito life cycle.

Table 2 FAIRE transgenic reporters
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Fig. 4
figure 4

FP DNA sequences promote gene expression in vivo. GFP reporter expression (from the reporters indicated in Table 2) was detected in the following D. melanogaster tissues: embryo (reporter A in a; whole-mount is shown), embryonic midgut (reporter B in b), third instar larval salivary gland (reporter C in c), pupal eyes (reporter D in d), third instar larval gut (reporter E in e), third instar larval fat body (reporter F in f), adult antenna (reporter G in g, reporter H in h), third instar larval denticle belts (reporter I in i), third instar larval leg discs (reporter J in j), and the third instar larval brain (reporter K in k)

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This investigation identified regulatory elements that drive gene expression in a wide variety of D. melanogaster tissues, including the eye (Fig. 4d), gut (Fig. 4b, e), salivary gland (Fig. 4c), fat body (Fig. 4f), olfactory system (Fig. 4g, h), denticle belts (Fig. 4i), leg imaginal discs (Fig. 4j), and brain (Fig. 4k). These studies in transgenic fruit flies provide functional validation of the FAIRE-seq data, suggesting that this investigation has succeeded in the goal of identifying regulatory elements. The Drosophila reporter assays permitted high throughput analysis of A. aegypti FAIRE-seq data in a manner which is not presently possible in mosquitoes, but which will lay the groundwork for full characterization of select CREs of interest directly in A. aegypti in future studies. To this end, we are presently pursuing a screen in Drosophila that aims to identify FP sequences that promote gene expression in tissues of vector importance (i.e., the olfactory system, midgut, salivary gland, and fat bodies). The elements selected for transgenic reporter studies presented here (Table 2, Fig. 4) were chosen based on their potential for driving gene expression in these tissues, which was estimated largely through examination of available Drosophila or mosquito gene expression data. Following the Drosophila screen, a number of regulatory elements that are confirmed to promote gene expression in tissues of interest will be tested directly in A. aegypti transgenics. It will be interesting to determine if these drivers promote comparable gene expression patterns in both D. melanogaster and A. aegypti. It is anticipated that if A. aegypti CREs drive tissue-specific reporter expression in Drosophila that mimics the activity of these elements in A. aegypti, the CREs are likely to function similarly in other dipterans, including additional vector mosquito species that are more closely related to A. aegypti than are fruit flies.

FPs mapping to UTRs

The 5’ UTR, the mRNA leader sequence directly upstream of the translation initiation codon, plays critical roles in the regulation of transcription as well as translation in eukaryotes [40]. The regulatory roles of 3’ UTRs are also well documented [41]. Using the UTR-Scan tool [30], a total of 405 and 764 FPs were identified in the 5’- (Additional file 4) and 3’- (Additional file 5) UTRs of genes, respectively. Interestingly, UTR-linked FPs appear to have a biased composition of known regulatory elements. Sequences representing the upstream open reading frame (uORF), Musashi-binding element (MBE), polyadenylation signal (PAS), and internal ribosome entry site (IRES) represented the most frequent regulators based on rank order analysis of their frequencies (Fig. 5). Interestingly, approximately half of all the UTR regulatory elements identified by UTRScan represented uORF elements. These elements, which are found in the 5' region of a mRNA transcript, are capable of regulating protein production and impact organismal development and growth in fungi, plants, and animals, including insects [42].

Fig. 5
figure 5

Known regulatory elements in UTR-linked FPs. Lower pie chart: uORF, MBE, PAS, and IRES motifs are the most frequent regulatory sequences observed in UTR-linked FP sequences. Upper pie chart: Infrequently observed UTR elements contained in the “Others” category of the lower pie chart are shown

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Intragenic regulators

Although intragenic regulators are significant in mammalian genomes [43, 44], such regulatory elements remain poorly understood in arthropods. However, a recent genome-wide study in D. melanogaster has revealed that many enhancers are localized within genes [45]. To know if FPs in A.aegypti coincide with intragenic enhancers, FPs localized within genes (Additional file 6) were analyzed. 767 FPs were identified in the exons of 744 genes (Additional file 7), and a total of 4434 identified FPs were localized within the intron sequences of 3747 genes (Additional file 8). 3991 FPs were identified in the exon-intron boundaries of 3592 genes (Additional file 9). The aggregated percentage of intragenic FPs is about 7.5 % of all the FPs identified in the genome. The relative distribution of FPs in upstream vs. intragenic sites is similar to the distribution bias of enhancer elements in D. melanogaster genes [45], indicating possible evolutionary conservation of intragenic regulation of genes in dipterans.

Association of FPs with non-coding genes

Major classes of A. aegypti non-coding genes including tRNA, rRNA, snRNA, and microRNAs, also have FPs within 1 kb upstream of their TSSs (Fig. 6; Additional file 10). The precursor transcripts of miRNA genes are known to be regulated by cis-acting elements [46, 47], thus suggesting that FPs identified within 1 kb upstream of 43 A. aegypti miRNA genes may play a significant role in regulating synthesis of their precursors. Similarly, specific sequences upstream of tRNA genes are also known to regulate synthesis of tRNA molecules [48]. 287 FPs are associated with upstream sequences within 1 kb of ~30 % of A. aegypti tRNA genes, indicating possible cis-regulation of isoacceptor tRNAs. In addition to miRNA and tRNA, snRNA and rRNA genes have also been found to have association with cis-acting regulators [49, 50], and FPs were found to flank these genes in A. aegypti.

Fig. 6
figure 6

FPs upstream of non-coding A. aegypti genes. tRNA, rRNA, snRNA, and microRNAs have FPs within 1 kb upstream of their TSSs

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Genetic variation in the regulatory regions of DENV susceptible and refractory mosquito strains

Identifying and understanding genetic variation in non-coding regions, which is often challenging even in genetic model organisms, is particularly difficult in non-models. In a recent study, Illumina sequencing of A. aegypti Moyo-S and Moyo-R genomic DNA revealed genome-wide genetic variation between the two strains [14–16]. In total, 13,158 high quality SNPs distributed throughout the genome (1321 supercontigs) were identified. These data were used to map the SNPs to FP sequences identified in the present study. The mapping data revealed that 3365 SNPs reside in FP DNA sequences (Additional file 11). To determine if there was any assortment bias of FAIRE-associated SNPs, bootstrap randomized sampling was performed as described earlier [51] in which 10,000 FPs were randomly sampled to determine how many contained a SNP. Performing a 100,000 randomized sampling, it was determined that on average, 28 SNPs were detectable in every 1000 FPs (Additional file 12). This pattern was consistent in each repeat of randomization, clearly suggesting no bias in the assortment of SNPs in the FPs.

Although several intensive studies have assessed SNPs in A. aegypti in a genome-wide manner [52–54], our studies are to our knowledge the first attempt to map A. aegypti SNPs to regulatory sequences across the genome. Likewise, this investigation is to our knowledge the first to map genetic polymorphism data from pathogen susceptible and refractory host strains to non-coding regulatory regions across the genome of the host. FAIRE-seq could be used for comparable analyses in malaria vector mosquitoes or hosts for other disease-causing pathogens. As discussed by Meyer and Thye [55], the next steps will be to identify causative genetic variation and the functionality of associated factors. To this end, SNPs in putative A. aegypti regulatory sequences [14–16] that flank genes with differential transcriptome profiles in Moyo-S vs. Moyo-R strains are presently being functionally characterized.

Conclusions

The results of this investigation indicate that FAIRE-seq is a powerful tool for identification of regulatory DNA in the genomes of non-model or emerging model organisms, including human disease vector mosquitoes. In this study, FAIRE-seq analysis of open chromatin in A. aegypti embryos permitted genome-wide discovery of >121,000 regulatory elements throughout the genome of A. aegypti. Many of these sequences clustered in the 1 kb 5’ upstream flanking regions of genes known to be expressed at this stage. Known transcription factor consensus binding sites were enriched in the FPs, and all of the elements tested in vivo were confirmed to drive reporter gene expression in assays conducted in D. melanogaster transgenic animals. Of the >13,000 single nucleotide polymorphisms (SNPs) recently identified in DENV-susceptible and refractory mosquito strains, over one-quarter mapped to FPs, suggesting that genetic variation in regulatory sequences may contribute to the susceptibility/refractoriness of A. aegypti strains to DENV infection.

Supporting data

Data sets supporting the results of this article are available within the article and its additional files, in the Sequence Read Archive (SRA) repository [56] (accession numbers SRP063665: http://www.ncbi.nlm.nih.gov/sra/SRP063665%5Baccn%5D, SRX1046562: http://www.ncbi.nlm.nih.gov/sra/SRX1046562%5Baccn%5D, and SRX1046561: http://www.ncbi.nlm.nih.gov/sra/SRX1046561%5Baccn%5D), and in the VB Genome Browser. FPs can be visualized with the VB Genome Browser at the following link:http://www.vectorbase.org/Aedes_aegypti/Location/View?db=core;r=supercont1.114:1965092-2050094;contigviewbottom=PRJNA294762_FAIREseq_Scheel_AaegL3=tiling. Access to the A. aegypti scaffolds reference v.4 to which these FPs were mapped is available at: https://www.vectorbase.org/downloadinfo/aedes-aegypti-liverpoolscaffoldsaaegl3fagz.