Abstract
The bacterial agglutination test (BAT) and the hemolysis inhibition test (HIT) were employed and evaluated.
The threshold value between positive and negative antibody titres in BAT and HIT was determined on the basis of titration results for sera from 25 adult goats infected with Corynebacterium pseudotuber-culosis (positive), and sera from 25 adult goats from a herd in which caseous lymphadenitis did not occur (negative).
Antibody titres were expressed as logio to the reciprocal value of the highest positive serum dilution in both tests. Positive titre (T) in BAT was stipulated as T ≥ 2.1 and in HIT as T > 0.6. The sensitivity and specificity was 0.96 in both tests in the material used. Twenty-three of the 25 goats infected with G. pseudotuberculosis were positive in both BAT and HIT.
The reproducibility in both tests was described by the use of correlation coefficient and coefficient of variation. The values were estimated using duplicate determination of titres of 155 serum samples. Correlation coefficients were 0.87 for BAT and 0.95 for HIT. Coef-ficients of variation were 26.4 % for BAT and 14.1 % for HIT. The coefficient of variation was related to titres. It was highest for BAT at low titres.
It was concluded that both tests can be used for seroepidemio-logical investigations of C. pseudotuberculosis infection in goats.
Sammendrag
Bakterieagglutinasjonstest (BAT) og antihemolysintest (AHT) ble etablert og anvendbarheten av testene vurdert.
Serumprøver fra 25 voksne geiter som med sikkerhet var infisert med Corynebacterium pseudotuberculosis ble benyttet som „sikre“ positive sera, og serumprøver fra 25 voksne geiter fra en besetning antatt fri for kaseøs lymfadenitt ble benyttet som „sikre“ negative sera for å fastsette grensen mellom positive og negative titer ved testene. Titeret ble angitt som logl0 til den resiproke verdi av høyeste seramfortynning som ga agglutinasjon ved BAT og høyeste serumfortynning uten hemolyse ved AHT. Titer lik 2.1 eller høyere ved BAT og lik 0.6 eller høyere ved AHT ble fastsatt som positive.
Sensitiviteten og spesifisiteten ved begge testene var lik 0.96 i det benyttede materialet.
Av 25 dyr hvorfra Corynebacterium pseudotuberculosis ble påvist var 23 positive ved bade BAT og AHT.
Som mal på reproduserbarheten til testene ble korrelasjonskoeffisienten og variasjonskoeffisienten benyttet. Parvise målinger av titeret i 155 sera ble foretatt. Korrelasjonskoeffisienten var 0.87 for BAT og 0.95 for AHT. Variasjonskoeffisienten var 26.4 % for BAT og 14.1 % for AHT. Variasjonskoeffisienten varierte med titeret. Den var høyest for BAT ved lave titer.
Det konkluderes med at begge testene kan brukes ved seroepidemiologiske undersøkelser av kaseøs lymfadenitt hos geit.
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Holstad, G. Corynebacterium Pseudotuberculosis Infection in Goats I.. Acta Vet Scand 27, 575–583 (1986). https://doi.org/10.1186/BF03548137
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DOI: https://doi.org/10.1186/BF03548137