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Signal-enhanced visual strand exchange amplification detection of African swine fever virus by the introduction of multimeric G-quadruplex/hemin DNAzyme

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Abstract

African swine fever virus (ASFV) causes hemorrhagic infectious disease in pigs with a fatality rate of nearly 100%. In this study, we developed a visual strand exchange amplification detection assay for ASFV. In the presence of ASFV, DNA amplification products containing multimeric G-quadruplex sequences were amplified by strand exchange amplification. These G-quadruplexes, assembled with hemin to form DNAzyme, displayed enhanced significant “turned-on” colorimetric signals to indicate detection results. The results showed that dimeric DNAzyme had the best visualization effect. Under the optimal reaction parameters, there was a linear relationship between the absorbance of the reaction solution at 417 nm and the logarithm of ASFV concentration ranged from 1 × 101 to 1 × 103 copies/μL, and the detection limit was 2.7 copies/μL. We hoped this visual assay could be helpful in the rapid and sensitive detection of ASFV, and the results of multimeric G-quadruplex/hemin DNAzyme could be helpful for the development of better visual detection assays.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31972158).

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Author notes

  1. Xianyong Wu and Qiming Chen have contributed equally to this work.

Authors and Affiliations

  1. School of Life Sciences, Shanghai University, Shanghai, 200444, China

    Xianyong Wu, Qiming Chen, Yuhao Huang, Qiqi Ning, Yingying Wang, Yilu Wang & Zhanmin Liu

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  1. Xianyong Wu
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  2. Qiming Chen
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Correspondence to Zhanmin Liu.

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Wu, X., Chen, Q., Huang, Y. et al. Signal-enhanced visual strand exchange amplification detection of African swine fever virus by the introduction of multimeric G-quadruplex/hemin DNAzyme. ANAL. SCI. 38, 675–682 (2022). https://doi.org/10.1007/s44211-022-00087-6

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  • DOI: https://doi.org/10.1007/s44211-022-00087-6

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