Skip to main content
SpringerLink
Log in

UV-induced skin’s green autofluorescence is a biomarker for both non-invasive evaluations of the dosages of UV exposures of the skin and non-invasive prediction of UV-induced skin damage

  • Original Papers
  • Published:
Photochemical & Photobiological Sciences Aims and scope Submit manuscript

Abstract

It is crucial to discover biomarkers for non-invasive evaluations of the dosages of UV exposures to a person during post-UV exposure period, and for non-invasive prediction of UV-induced skin damage. Our current study has obtained findings: UVB exposures produced dose-dependent increases in skin’s green autofluorescence (AF) intensity of mice, which were significantly associated with the UVB dosages. The UVC-induced green AF increases were dose dependent, which were highly associated with the UVC dosages. Moreover, both previous reports and our current study have collectively shown significant association between UVB/UVC dosages and UVB/UVC-induced skin damage. Collectively, our study has indicated that the UVB/UVC-induced skin’s AF are first biomarkers for both non-invasive evaluations of the dosages of UV exposures to a person during post-UV exposure period and non-invasive and label-free prediction of UVB/UVC-induced skin damage.

Graphical abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Chen, H., Weng, Q. Y., & Fisher, D. E. (2014). UV signaling pathways within the skin. The Journal of Investigative Dermatology, 134(8), 2080–2085. https://doi.org/10.1038/jid.2014.161

    Article  CAS  Google Scholar 

  2. D’Orazio, J., et al. (2013). UV radiation and the skin. International Journal of Molecular Sciences, 14(6), 12222–12248. https://doi.org/10.3390/ijms140612222

    Article  CAS  Google Scholar 

  3. Elwood, J. M., & Jopson, J. (1997). Melanoma and sun exposure: An overview of published studies. International Journal of Cancer., 73(2), 198–203.

    Article  CAS  Google Scholar 

  4. de Jager, T. L., Cockrell, A. E., & Du Plessis, S. S. (2017). Ultraviolet light induced generation of reactive oxygen species. Advances in Experimental Medicine and Biology, 996, 15–23. https://doi.org/10.1007/978-3-319-56017-5_2

    Article  CAS  Google Scholar 

  5. Mullenders, L. H. F. (2018). Solar UV damage to cellular DNA: From mechanisms to biological effects. Photochemical & Photobiological Sciences, 17(12), 1842–1852. https://doi.org/10.1039/c8pp00182k

    Article  CAS  Google Scholar 

  6. Burke, K. E. (2018). Mechanisms of aging and development-A new understanding of environmental damage to the skin and prevention with topical antioxidants. Mechanisms of Ageing and Development, 172, 123–130. https://doi.org/10.1016/j.mad.2017.12.003

    Article  Google Scholar 

  7. Elwood, J. M., & Jopson, J. (1997). Melanoma and sun exposure: An overview of published studies. International Journal of Cancer, 73(2), 198–203. https://doi.org/10.1002/(sici)1097-0215(19971009)73:2%3c198::aid-ijc6%3e3.0.co;2-r

    Article  CAS  Google Scholar 

  8. Mohania, D., et al. (2017). Ultraviolet radiations: Skin defense-damage mechanism. Advances in Experimental Medicine and Biology, 996, 71–87. https://doi.org/10.1007/978-3-319-56017-5_7

    Article  CAS  Google Scholar 

  9. Roy, S. (2017). Impact of UV radiation on genome stability and human health. Advances in Experimental Medicine and Biology, 996, 207–219. https://doi.org/10.1007/978-3-319-56017-5_17

    Article  CAS  Google Scholar 

  10. Guerra, K. C., Zafar, N., & Crane, J. S. (2021). Skin cancer prevention. StatPearls. Treasure Island.

    Google Scholar 

  11. Sproul, C. D., et al. (2014). Cyclobutane pyrimidine dimer density as a predictive biomarker of the biological effects of ultraviolet radiation in normal human fibroblast. Photochemistry and Photobiology, 90(1), 145–154. https://doi.org/10.1111/php.12194

    Article  CAS  Google Scholar 

  12. Ikehata, H., et al. (2018). Quantitative analysis of UV photolesions suggests that cyclobutane pyrimidine dimers produced in mouse skin by UVB are more mutagenic than those produced by UVC. Photochemical & Photobiological Sciences, 17(4), 404–413. https://doi.org/10.1039/c7pp00348j

    Article  CAS  Google Scholar 

  13. Khalil, C., & Shebaby, W. (2017). UVB damage onset and progression 24 h post exposure in human-derived skin cells. Toxicology Reports, 4, 441–449. https://doi.org/10.1016/j.toxrep.2017.07.008

    Article  CAS  Google Scholar 

  14. ten Berge, O., et al. (2011). Assessment of cyclobutane pyrimidine dimers by digital photography in human skin. Journal of Immunological Methods, 373(1–2), 240–246. https://doi.org/10.1016/j.jim.2011.07.014

    Article  CAS  Google Scholar 

  15. Moran, C., et al. (2015). Type 2 diabetes, skin autofluorescence, and brain atrophy. Diabetes, 64(1), 279–283. https://doi.org/10.2337/db14-0506

    Article  CAS  Google Scholar 

  16. Varikasuvu, S. R., Aloori, S., & Bhongir, A. V. (2021). Higher skin autofluorescence detection using AGE-Reader technology as a measure of increased tissue accumulation of advanced glycation end products in dialysis patients with diabetes: A meta-analysis. Journal of Artificial Organs, 24(1), 44–57. https://doi.org/10.1007/s10047-020-01189-6

    Article  CAS  Google Scholar 

  17. Bos, D. C., de Ranitz-Greven, W. L., & de Valk, H. W. (2011). Advanced glycation end products, measured as skin autofluorescence and diabetes complications: A systematic review. Diabetes Technology & Therapeutics, 13(7), 773–779. https://doi.org/10.1089/dia.2011.0034

    Article  CAS  Google Scholar 

  18. Pena, A., et al. (2005). Spectroscopic analysis of keratin endogenous signal for skin multiphoton microscopy. Optics Express, 13(16), 6268–6274.

    Article  CAS  Google Scholar 

  19. Bader, A. N., et al. (2011). Fast nonlinear spectral microscopy of in vivo human skin. Biomedical Optics Express, 2(2), 365–373. https://doi.org/10.1364/BOE.2.000365

    Article  Google Scholar 

  20. Sheng, C., et al. (2012). NAD+ administration significantly attenuates synchrotron radiation X-ray-induced DNA damage and structural alterations of rodent testes. International Journal of Physiology, Pathophysiology and Pharmacology, 4(1), 1–9.

    CAS  Google Scholar 

  21. Hennings, L., et al. (2009). Dead or alive? Autofluorescence distinguishes heat-fixed from viable cells. International Journal of Hyperthermia., 25(5), 355–363.

    Article  Google Scholar 

  22. Kozlova, A. A., et al. (2020). Changes in autofluorescence level of live and dead cells for mouse cell lines. Journal of Fluorescence., 30(6), 1–7.

    Article  Google Scholar 

  23. Kang, S. W., et al. (2000). Antisense oligonucleotide of clusterin mRNA induces apoptotic cell death and prevents adhesion of rat ASC-17D Sertoli cells. Molecules and Cells, 10(2), 193–198. https://doi.org/10.1007/s10059-000-0193-3

    Article  CAS  Google Scholar 

  24. Narayanan, D. L., Saladi, R. N., & Fox, J. L. (2010). Ultraviolet radiation and skin cancer. International Journal of Dermatology, 49(9), 978–986. https://doi.org/10.1111/j.1365-4632.2010.04474.x

    Article  Google Scholar 

  25. Zhou, Y., et al. (2021). Photoprotective effect of artemisia sieversiana ehrhart essential oil against UVB-induced photoaging in mice. Photochemistry and Photobiology. https://doi.org/10.1111/php.13561

    Article  Google Scholar 

  26. Park, H. S., et al. (2014). Toll-like receptor 2 mediates a cutaneous reaction induced by repetitive ultraviolet B irradiation in C57/BL6 mice in vivo. Experimental Dermatology., 23, 8.

    Article  Google Scholar 

  27. Dimitrios, et al. (2014). In-vivo imaging of psoriatic lesions with polarization multispectral dermoscopy and multiphoton microscopy. Biomedical Optics Express., 2, 2.

    Google Scholar 

  28. Beermann, F., et al. (1990). Rescue of the albino phenotype by introduction of a functional tyrosinase gene into mice. EMBO Journal, 9(9), 2819–2826.

    Article  CAS  Google Scholar 

  29. Patterson, G. H., et al. (2000). Separation of the glucose-stimulated cytoplasmic and mitochondrial NAD(P)H responses in pancreatic islet beta cells. Proceedings of the National Academy of Sciences of the United States of America, 97(10), 5203–5207. https://doi.org/10.1073/pnas.090098797

    Article  CAS  Google Scholar 

  30. Georgakoudi, I., et al. (2002). NAD(P)H and collagen as in vivo quantitative fluorescent biomarkers of epithelial precancerous changes. Cancer Research., 62(3), 682–687.

    CAS  Google Scholar 

  31. Nogueira, M.S. and C. Kurachi. Assessing the photoaging process at sun exposed and non-exposed skin using fluorescence lifetime spectroscopy. in Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis. 2016.

  32. Andrei, et al. (2008). Imaging of zinc oxide nanoparticle penetration in human skin in vitro and in vivo. Journal of Biomedical Optics., 13(6), 064031.

    Article  Google Scholar 

Download references

Acknowledgements

The authors would like to acknowledge the financial support by two research grants from a Major Special Program Grant of Shanghai Municipality (Grant # 2017SHZDZX01) (to W.Y.).

Author information

Authors and Affiliations

  1. School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai, 200030, People’s Republic of China

    Mingchao Zhang & Weihai Ying

  2. Multiscale Research Institute of Complex Systems, Fudan University, 220 Handan Road, Shanghai, People’s Republic of China

    Mingchao Zhang

  3. Collaborative Innovation Center for Genetics and Development, Shanghai, 200043, People’s Republic of China

    Weihai Ying

Authors
  1. Mingchao Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Weihai Ying
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Weihai Ying.

Supplementary Information

Rights and permissions

Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, M., Ying, W. UV-induced skin’s green autofluorescence is a biomarker for both non-invasive evaluations of the dosages of UV exposures of the skin and non-invasive prediction of UV-induced skin damage. Photochem Photobiol Sci 22, 159–168 (2023). https://doi.org/10.1007/s43630-022-00306-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s43630-022-00306-z

Keywords

Search

Navigation