Skip to main content
SpringerLink
Log in

Characterization of nanosized hydroxyapatite prepared by an aqueous precipitation method using eggshells and mulberry leaf extract

  • Original Article
  • Published:
Journal of the Korean Ceramic Society Aims and scope Submit manuscript

Abstract

Hydroxyapatite (HA) has been vigorously studied for orthopedic and dental applications due to its excellent bioactivity, osteoconductivity, and osteoinductivity. This study aims to present a simple room-temperature aqueous precipitation method for obtaining carbonated HA nanoparticles of high purity from biowaste chicken eggshells. Biowaste chicken eggshells were used for preparing HA through aqueous precipitation method. Moreover, mulberry leaf extract was used as a template to regulate the morphology, size, and crystallinity of HA. All produced nanocrystalline HA powders exhibited rod-like particle agglomerates of a size below 100 nm. The HA particles synthesized with the mulberry leaf extract showed higher crystallinity and slightly decreased crystallite sizes compared to the samples prepared without adding the extract. Carbonate peaks observed for the specimens closely matched those of A- and B-type carbonates, which can contribute to the low crystallinity. Notably, the HA synthesized from the eggshell powders contains several important trace elements such as Mg and Sr, which are beneficial to the overall biological performance as biomaterials. The morphology, size, structure, and composition of the obtained HA products are similar to those of natural bones, and consequently, the products show potential as a material for biomedical applications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. N. Kaneko, Y. Suzuki, R. Umeda, R. Namiki, C. Izawa, T.I. Fukazawa, M. Honda, T. Takei, T. Watanabe, M. Aizawa, J. Asian Ceram. Soc. 8, 130 (2020)

    Article  Google Scholar 

  2. L.L. Hench, J. Am. Ceram. Soc. 74, 1487 (1991)

    Article  CAS  Google Scholar 

  3. A.E. Khouri, A. Zegzouti, M. Elaatmani, F. Capitelli, Inorg. Chem. Commun. 110, 107568 (2019)

    Article  CAS  Google Scholar 

  4. S. Dasgupta, S. Tarafder, A. Bandyopadhyay, S. Bose, Mater. Sci. Eng. C 33, 2846 (2013)

    Article  CAS  Google Scholar 

  5. A.P. Shpak, V.L. Karbovskii, A.G. Vakh, J. Electron. Spectrosc. 585, 137 (2004)

    Google Scholar 

  6. S. Kannan, A. Rebelo, J.M.F. Ferreira, J. Inorg. Biochem. 100, 1692 (2006)

    Article  CAS  Google Scholar 

  7. W. Kong, K. Zhao, C. Gao, Mater. Lett. 255, 126552 (2019)

    Article  CAS  Google Scholar 

  8. G. Zuo, Y. Wan, L. Wang, C. Liu, F. He, H. Luo, Mater. Lett. 64, 2126 (2010)

    Article  CAS  Google Scholar 

  9. P. Zhang, Z. Hong, T. Yu, X. Chen, X. Jing, Biomaterials 30, 58 (2009)

    Article  CAS  Google Scholar 

  10. C.-H. Hou, S.-M. Hou, Y.-S. Hsueh, J. Lin, H.-C. Wu, F.-H. Lin, Biomaterials 30, 3956 (2009)

    Article  CAS  Google Scholar 

  11. S.C. Wu, H.C. Hsu, Y.N. Wu, W.F. Ho, Mater. Character. 62, 1180 (2011)

    Article  CAS  Google Scholar 

  12. W.F. Ho, H.C. Hsu, S.K. Hsu, C.W. Hung, S.C. Wu, Ceram. Inter. 39, 6467 (2013)

    Article  CAS  Google Scholar 

  13. S.C. Wu, H.C. Hsu, S.K. Hsu, Y.C. Chang, W.F. Ho, J. Asian Ceram. Soc. 4, 85 (2016)

    Article  Google Scholar 

  14. D. Liu, T. Troczynski, W.J. Tseng, Ceram. Inter. 22, 1721 (2001)

    CAS  Google Scholar 

  15. S.C. Wu, H.K. Tsou, H.C. Hsu, S.K. Hsu, S.P. Liou, W.F. Ho, Ceram. Inter. 39, 8183 (2013)

    Article  CAS  Google Scholar 

  16. N. Roveri, G. Falini, M.C. Sidoti, A. Tampieri, E. Landi, M. Sandri, B. Pama, Mater. Sci. Eng. C 23, 441 (2003)

    Article  CAS  Google Scholar 

  17. X. Liu, L.A. Smith, J. Hu, P.X. Ma, Biomaterials 30, 2252 (2009)

    Article  CAS  Google Scholar 

  18. M. Gungormus, M. Branco, H. Fong, J.P. Schneider, C. Tamerler, M. Sarikaya, Biomaterials 31, 7266 (2010)

    Article  CAS  Google Scholar 

  19. K. Doi, T. Kojima, Y. Fujimoto, Biol. Pharm. Bull. 23, 1066 (2000)

    Article  CAS  Google Scholar 

  20. Y. Shibata, N. Kume, H. Arai, K. Hayashida, A. Inui-Hayashida, M. Minami, E. Mukai, M. Toyohara, A. Harauma, T. Murayama, T. Kita, S. Hara, K. Kamei, M. Yokode, Atherosclerosis 193, 20 (2007)

    Article  CAS  Google Scholar 

  21. E. Landi, A. Tampieri, G. Celotti, S. Sprio, J. Eur. Ceram. Soc. 20, 2377 (2000)

    Article  CAS  Google Scholar 

  22. M.H. Fathia, A. Hanifia, V. Mortazavi, J. Mater. Process. Technol. 202, 536 (2008)

    Article  CAS  Google Scholar 

  23. H.M. Kim, Y. Kim, S.J. Park, C. Rey, H.M. Lee, M.J. Gimcher, J.S. Ko, Biomaterials 21, 1129 (2000)

    Article  CAS  Google Scholar 

  24. M.S. Sadjadi, M. Meskinfam, B. Sadeghi, H. Jazdarreh, K. Zare, Mater. Chem. Phys. 124, 217 (2010)

    Article  CAS  Google Scholar 

  25. B. Ebrahimi-Kahrizsangi, A. Nasiri-Tabrizi, Chami. Solid State Sci. 12, 1645 (2010)

    Article  CAS  Google Scholar 

  26. X. Zhu, Y. Masuda, K. Koumoto, Biomaterials 25, 3915 (2004)

    Article  CAS  Google Scholar 

  27. K.D. Son, Y.-J. Kim, Mater. Sci. Eng. C 33, 499 (2013)

    Article  CAS  Google Scholar 

  28. Y. Deng, Y. Sun, X. Chen, P. Zhu, S. Wei, Mater. Sci. Eng. C 33, 2905 (2013)

    Article  CAS  Google Scholar 

  29. S. Mollazadeh, J. Javadpour, A. Khavandi, Ceram. Inter. 33, 1579 (2007)

    Article  CAS  Google Scholar 

  30. G. Balasundaram, M. Sato, T.J. Webster, Biomaterials 27, 2798 (2006)

    Article  CAS  Google Scholar 

  31. R. Murugan, S. Ramakrishna, Acta Biomater. 2, 201 (2006)

    Article  CAS  Google Scholar 

  32. S. Cazalbou, C. Combes, D. Eichert, C. Rey, J. Mater. Chem. 14, 2148 (2004)

    Article  CAS  Google Scholar 

  33. W.L. Suchanek, K. Byrappa, P. Shuk, R.E. Riman, V.F. Janas, K.S. TenHuisen, Biomaterials 25, 4647 (2004)

    Article  CAS  Google Scholar 

  34. A. Hoppe, N.S. Güldal, A.R. Boccaccini, Biomaterials 32, 2757 (2011)

    Article  CAS  Google Scholar 

  35. J. Braux, F. Velard, C. Guillaume, S. Bouthors, E. Jallot, J.M. Nedelec, D. Laurent-Maquin, P. Laquerrière, Acta Biomater. 7, 2593 (2011)

    Article  CAS  Google Scholar 

  36. C. Capuccini, P. Torricelli, F. Sima, E. Boanini, C. Ristoscu, B. Bracci, G. Socol, M. Fini, I.N. Mihailescu, A. Bigi, Acta Biomater. 4, 1885 (2008)

    Article  CAS  Google Scholar 

  37. E. Gentleman, Y.C. Fredholm, G. Jell, N. Lotfibakhshaiesh, M.D. O’Donnell, R.G. Hill, M.M. Stevens, Biomaterials 31, 3949 (2010)

    Article  CAS  Google Scholar 

  38. K. Prabakaran, S. Rajeswari, Spectrochim. Acta A 74, 1127 (2009)

    Article  CAS  Google Scholar 

  39. S. Koutsopoulos, J. Biomed. Mater. Res. 62, 600 (2002)

    Article  CAS  Google Scholar 

  40. I.K. Januariyasa, I.D. Ana, Y. Yusuf, Mater. Sci. Eng. C 107, 110347 (2020)

    Article  CAS  Google Scholar 

  41. A. Matsuura, T. Kubo, K. Doi, K. Hayashi, K. Morita, R. Yokota, H. Hayashi, I. Hirata, M. Okazaki, Y. Akagawa, Dent. Mater. J. 28, 234 (2009)

    Article  CAS  Google Scholar 

  42. S.J. Kalita, S. Verma, Mater. Sci. Eng. C 30, 295 (2010)

    Article  CAS  Google Scholar 

  43. M. Tămăşan, L.S. Ozyegin, F.N. Oktar, V. Simon, Mater. Sci. Eng. C 33, 2569 (2013)

    Article  CAS  Google Scholar 

  44. J. Peña, M. Vallet-Regí, J. Eur. Ceram. Soc. 23, 1687 (2003)

    Article  CAS  Google Scholar 

  45. R. Enderle, F. Götz-Neunhoeffer, M. Göbbels, F.A. Müller, P. Greil, Biomaterials 26, 3379 (2005)

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors acknowledge the partial financial support of Ministry of Science and Technology of Taiwan (105-2218-E-390-002; 106-2218-E-390-001; 107-2218-E-390-001; 107-2218-E-390-007; 108-2218-E-390-002).

Author information

Authors and Affiliations

  1. Department of Dental Technology and Materials Science, Central Taiwan University of Science and Technology, Taichung, 40601, Taiwan

    Shih-Ching Wu & Hsueh-Chuan Hsu

  2. Department of Chemical and Materials Engineering, National University of Kaohsiung, 700 Kaohsiung University Rd., Nanzih District, Kaohsiung, 81148, Taiwan

    Mei-Yi Liu & Wen-Fu Ho

Authors
  1. Shih-Ching Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hsueh-Chuan Hsu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mei-Yi Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wen-Fu Ho
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wen-Fu Ho.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wu, SC., Hsu, HC., Liu, MY. et al. Characterization of nanosized hydroxyapatite prepared by an aqueous precipitation method using eggshells and mulberry leaf extract. J. Korean Ceram. Soc. 58, 116–122 (2021). https://doi.org/10.1007/s43207-020-00087-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s43207-020-00087-0

Keywords

Search

Navigation