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HDAC6 Is Involved in the Histone Deacetylation of In Vitro Maturation Oocytes and the Reprogramming of Nuclear Transplantation in Pig

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Abstract

It remained unknown whether HDAC6 affected the histone deacetylation of in vitro maturation oocytes and the reprogramming of nuclear transplantation in pig. Our results indicated that HDAC6 specific inhibition did not affect overall HDAC activity and meiosis process, which increased histone H3K9/K14 and H4K8 acetylation of porcine in vitro maturation oocytes and pseudo-pronucleus embryos. HDAC6 inhibition also significantly enhanced the cleavage and blastocyst of nuclear transfer embryos (0.81 ± 0.12 vs. 0.68 ± 0.12 and 0.46 ± 0.19; 0.73 ± 0.13 vs. 0.63 ± 0.18 and 0.40 ± 0.16, P<0.05). The inhibition of HDAC6 significantly enhanced histone H3K9/K14 and H4K8 acetylation, and upregulated the OCT4 and CDX2 expressions (1.83 ± 0.16 vs. 1.00 ± 0.00 %; 2.07 ± 0.09 vs. 1.00 ± 0.00; P<0.05) in porcine SCNT blastocysts. Interestingly, HDAC6 inhibition significantly increased the pseudo-pronucleus volume during somatic cell reprogramming. Thus, HDAC6 was required for porcine histone deacetylation during the in vitro maturation and pseudo-pronucleus stages. HDAC6 inhibition improved the in vitro development of nuclear transfer embryos. HDAC6 may restrict the reprogramming of somatic nuclear transfer by regulating pseudo-pronucleus expansion. We need further research to confirm this in the future.

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Acknowledgements

These experiments were supported from the China National High Technology Research and Development Program (863) Project (2011AA100607), National Natural Science Foundation Project (31260552 and 31401267), Guangxi Natural Science Foundation (Grant No. 2014GXNSFCB118003), and Guangxi Medical University Youth Science Foundation Project (GXMUYSF201829).

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Authors and Affiliations

  1. Laboratory Animal Center, Guangxi Medical University, Nanning, 530021, Guangxi, China

    JunMing Sun

  2. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, 530005, Guangxi, China

    QingYou Liu, Zhi Peng Li, Ben Huang, KuiQing Cui & DeShun Shi

  3. Guangxi Key Laboratory of Livestock Genetic Improvement, Guangxi Institute of Animal Sciences, Nanning, 530001, Guangxi, China

    LingYan Lv & RuYu Sun

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  1. JunMing Sun
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Correspondence to JunMing Sun, KuiQing Cui or DeShun Shi.

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All experiments were conducted in accordance with the guiding principles of the ethical review committee of Guangxi Medical University.

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Sun, J., Liu, Q., Lv, L. et al. HDAC6 Is Involved in the Histone Deacetylation of In Vitro Maturation Oocytes and the Reprogramming of Nuclear Transplantation in Pig. Reprod. Sci. 28, 2630–2640 (2021). https://doi.org/10.1007/s43032-021-00533-2

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  • DOI: https://doi.org/10.1007/s43032-021-00533-2

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