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LncRNA H19 Overexpression in Endometriosis and its Utility as a Novel Biomarker for Predicting Recurrence

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Abstract

Endometriosis has a high recurrence rate after treatment, and there are no effective recurrence predictors. LncRNA H19 has been found to be a predictor of the poor prognosis of multiple diseases. Here, we aimed to investigate the expression and clinical implications of lncRNAH19 in endometriosis and explore the clinical application value of lncRNAH19 for recurrence prediction. LncRNA H19 expression was evaluated in 104 ectopic and eutopic endometrial samples from patients with endometriosis and 50 control endometrial samples from patients without endometriosis. The association between lncRNA H19 expression and the clinical characteristics of endometriosis as well as its value as a potential predictor of recurrence were analyzed. LncRNA H19 expression in the ectopic and eutopic endometria of endometriosis patients was significantly higher than that in the normal endometrium. LncRNA H19 expression in the ectopic endometrium was associated with infertility, recurrence, bilateral ovarian lesions, an increased CA125 level, and revised American Fertility Society (rAFS) stage. Multivariate logistic regression analysis showed that age less than 40 years and lncRNA H19 overexpression in the ectopic endometrium were independent prognostic factors of endometriosis recurrence, and the receiver operating characteristic (ROC) curve showed that the sensitivity and specificity for predicting recurrence were 90.9% and 61.0%, respectively, when the lncRNA H19 expression level in the ectopic endometrium was higher than 0.0277. LncRNA H19 may be involved in the pathogenesis of endometriosis especially in the mechanism of recurrence and is a novel potential predictor of the recurrence of endometriosis.

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Availability of Supporting Data

The supporting data used or analyzed during the current study are available from the corresponding author on reasonable request.

Funding

This study was supported by the Key Talents Project of Maternal and Child Health of Jiangsu (No. FRC201730; to Songping Liu­), Key Science and Technology (Social Development) Project of Zhenjiang (No. SH2019062; to Songping Liu­), Project of Jiangsu Commission of Health (No. LGY2019027; to Songping Liu­), Six Talent Peaks Project of Jiangsu (No. 2015‑WSW‑088; to Songping Liu­), the National Natural Science Foundation of China (No. 81971361; to Junjun Qiu), the Natural Science Foundation of Shanghai Science and Technology (No. 19ZR1406900; to Junjun Qiu), and shanghai “Rising Stars of Medical Talent” Youth Development Program to Junjun Qiu.

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Author notes

  1. Songping Liu, Weijuan Xin, Xiaoyan Tang and Junjun Qiu contributed equally to this work.

Authors and Affiliations

  1. Department of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, 128 Shenyang RD, Shanghai, 200090, China

    Songping Liu, Weijuan Xin, Xiaoyan Tang, Junjun Qiu & Keqin Hua

  2. Department of Obstetrics and Gynecology, Zhenjiang Maternal and Child Health Hospital, 20 Zhengdong RD, Zhenjiang, Jiangsu, 212001, China

    Yajiao Zhang

Authors
  1. Songping Liu
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  2. Weijuan Xin
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  4. Junjun Qiu
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  6. Keqin Hua
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Contributions

S.P.L. and K.Q.H. conceived and designed the study. S.P.L., X.Y.T., J.J.Q., and Y.J.Z. performed the experiments. S.P.L. and X.Y.T. wrote the manuscript. S.P.L. and W.J.X analyzed the data and revised the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Keqin Hua.

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Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Approval and Consent to Participate

Institutional review board approval was received from Zhenjiang Maternal and Child Health Hospital, and all patients provided informed consent for the investigation and experiments.

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Liu, S., Xin, W., Tang, X. et al. LncRNA H19 Overexpression in Endometriosis and its Utility as a Novel Biomarker for Predicting Recurrence. Reprod. Sci. 27, 1687–1697 (2020). https://doi.org/10.1007/s43032-019-00129-x

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  • DOI: https://doi.org/10.1007/s43032-019-00129-x

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