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Characterization of biosurfactant produced in response to petroleum crude oil stress by Bacillus sp. WD22 in marine environment

  • Environmental Microbiology - Research Paper
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Abstract

Bacillus sp. WD22, previously isolated from refinery effluent, degraded 71% of C8 hydrocarbons present in 1.0% v/v PCO in seawater (control medium), which reduced to 16.3%, on addition of yeast extract. The bacteria produced a biosurfactant in both media, whose surface was observed to be amorphous in nature under FESEM-EDAX analysis. The biosurfactant was characterized as a linear surfactin by LCMS and FT-IR analysis. The critical micelle concentration was observed as 50 mg/L and 60 mg/L at which the surface tension of water was reduced to 30 mN/m. Purified biosurfactant could emulsify petroleum-based oils and vegetable oils effectively and was stable at all tested conditions of pH, salinity and temperature up to 80 °C. The biosurfactant production was found to be mixed growth associated in control medium, while it was strictly growth associated in medium with yeast extract as studied by the Leudeking-Piret model.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

LCG thanks the Vision Group of Science and Technology, Govt. of Karnataka (VGST-GoK) for funding this research work under RGS-F grant (GRD No 846/315).

Funding

This work is funded by the Vision Group of Science and Technology, Govt. of Karnataka (VGST-GoK) RGS-F grant (GRD No 846/315).

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Authors and Affiliations

  1. Department of Biotechnology Engineering, NMAM Institute of Technology-Affiliated to NITTE (Deemed to Be University), Nitte, Karnataka, 574110, India

    Louella Concepta Goveas & Shyama Prasad Sajankila

  2. Department of Chemical Engineering, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India

    Raja Selvaraj

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  1. Louella Concepta Goveas
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  2. Raja Selvaraj
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  3. Shyama Prasad Sajankila
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Contributions

Louella Concepta Goveas: Conceptualization, methodology, validation, investigation, writing—original draft, writing—review and editing. Shyama Prasad Sajankila: Methodology, supervision. Raja Selvaraj: Validation, supervision.

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Correspondence to Louella Concepta Goveas.

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Goveas, L.C., Selvaraj, R. & Sajankila, S.P. Characterization of biosurfactant produced in response to petroleum crude oil stress by Bacillus sp. WD22 in marine environment. Braz J Microbiol 53, 2015–2025 (2022). https://doi.org/10.1007/s42770-022-00811-4

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