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Cadmium-tolerant endophytic Pseudomonas rhodesiae strains isolated from Typha latifolia modify the root architecture of Arabidopsis thaliana Col-0 in presence and absence of Cd

  • Environmental Microbiology - Research Paper
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Abstract

In this work, we isolated four Cd-tolerant endophytic bacteria from Typha latifolia roots that grow at a Cd-contaminated site. Bacterial isolates GRC065, GRC066, GRC093, and GRC140 were identified as Pseudomonas rhodesiae. These bacterial isolates tolerate cadmium and have abilities for phosphate solubilization, siderophore production, indole acetic acid (IAA) synthesis, and ACC deaminase activity, suggesting that they are plant growth-promoting rhizobacteria. Bacterial inoculation in Arabidopsis thaliana seedlings showed that P. rhodesiae strains increase total fresh weight and number of lateral roots concerning non-inoculated plants. These results indicated that P. rhodesiae strains promote A. thaliana seedlings growth by modifying the root system. On the other hand, in A. thaliana seedlings exposed to 2.5 mg/l of Cd, P. rhodesiae strains increased the number and density of lateral roots concerning non-inoculated plants, indicating that they modify the root architecture of A. thaliana seedlings exposed to cadmium. The results showed that P. rhodesiae strains promote the development of lateral roots in A. thaliana seedlings cultivated in both conditions, with and without cadmium. These results suggest that P. rhodesiae strains could exert a similar role inside the roots of T. latifolia that grow in the Cd-contaminated environment.

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Funding

The work reported was funded by grants from CONACYT (Fondo Sectorial de Investigación para la Educación, CB2017-2018 A1-S-40454), Fondo de Apoyo a la Investigación (UASLP 2019 C19-FAI-05-40.40), and Fondos Concurrentes de la UASLP (FCR UASLP 210920280) to Alejandro Hernández-Morales. Gisela Adelina Rolón-Cárdenas (CVU 712240) thanks CONACYT-Mexico for the financial support given to carry out her Ph.D. studies.

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Authors and Affiliations

  1. Posgrado en Ciencias Químicas, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, San Luis Potosí, San Luis Potosí, Mexico

    Gisela Adelina Rolón-Cárdenas & Alejandro Hernández-Morales

  2. Facultad de Estudios Profesionales Zona Huasteca, Universidad Autónoma de San Luis Potosí, Romualdo del Campo 501, Fraccionamiento Rafael Curiel, CP 79060, Ciudad Valles, San Luis Potosí, Mexico

    Gisela Adelina Rolón-Cárdenas & Alejandro Hernández-Morales

  3. Secretaría de Investigación y Posgrado, Centro Nayarita de Innovación y Transferencia de Tecnología (CENITT), Universidad Autónoma de Nayarit, Tepic, Nayarit, Mexico

    Jackeline Lizzeta Arvizu-Gómez

  4. Facultad de Química, Universidad Autónoma de Querétaro, Querétaro, Querétaro, Mexico

    Juan Ramiro Pacheco-Aguilar

  5. Departamento de Ingeniería Bioquímica, Tecnológico Nacional de México (TecNM), Instituto Tecnológico Superior de Irapuato, Irapuato, Guanajuato, Mexico

    Juan Vázquez-Martínez

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  1. Gisela Adelina Rolón-Cárdenas
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  5. Alejandro Hernández-Morales
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Contributions

GARC (Ph. D. student) performed the research, analyzed data, and wrote the paper. JLAG, JRPA, and JVM participated in experimental design, contributed to bacterial characterization, and analyzed data. AHM conceived the study, analyzed data, and participated in the paper writing. All authors read and approved the final version of the manuscript.

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Correspondence to Alejandro Hernández-Morales.

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Rolón-Cárdenas, G.A., Arvizu-Gómez, J.L., Pacheco-Aguilar, J.R. et al. Cadmium-tolerant endophytic Pseudomonas rhodesiae strains isolated from Typha latifolia modify the root architecture of Arabidopsis thaliana Col-0 in presence and absence of Cd. Braz J Microbiol 52, 349–361 (2021). https://doi.org/10.1007/s42770-020-00408-9

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