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Cell wall glycolipids from Corynebacterium pseudotuberculosis strains with different virulences differ in terms of composition and immune recognition

  • Veterinary Microbiology - Research Paper
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Abstract

Caseous lymphadenitis (CLA) is an infectious disease caused by Corynebacterium pseudotuberculosis in small ruminants and is characterized by the development of granulomas in the lymph nodes, spleen, liver, and lungs. Although little is known about the host-pathogen relationship of this bacterium, it was previously reported that the pathogen’s lipids are important for its taxonomic classification and survival inside macrophages. However, there are no studies regarding the composition of these molecules. In this study, cell wall glycolipids from two C. pseudotuberculosis strains presenting different virulence profiles were purified and its composition was characterized. A difference was observed between the electrophoretic and chromatogram profiles for cell wall components from the two strains, mainly among molecules with low molecular weights. IgM from sheep with acute CLA recognized antigens with an estimated molecular weight of 11 kDa of the low-pathogenicity strain, while low-molecular weight antigens from the high-pathogenicity strain presented a lower recognition by these antibodies. Mass spectrometry analysis showed that the cell wall of the high-pathogenicity strain contained glycolipids with high amounts of unsaturated fatty acids and glycerophosphoinositols, which may contribute to the capacity of this strain to cause severe disease. In conclusion, it is indicated that cell wall non-protein antigens can play a key role in C. pseudotuberculosis virulence.

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Acknowledgments

The authors are grateful to Francisca Soares (Labimuno-UFBA) for technical assistance.

Funding

This work was funded by Fundação de Apoio à Pesquisa do Estado da Bahia (FAPESB) grant number PPP 022/2009. VA is a research fellow from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). RWP is a Technological Development fellow from CNPq (Proc. 313350 / 2019-1).

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Authors and Affiliations

  1. Laboratory of Immunology and Molecular Biology, Health Sciences Institute, Federal University of Bahia, Salvador, Bahia, 40110-100, Brazil

    Miriam Flores Rebouças, Dan Loureiro, Thiago Doria Barral, José Tadeu Raynal, Roberto Meyer & Ricardo Wagner Portela

  2. Post-graduation Program in Microbiology, Institute of Biology, Federal University of Bahia, Salvador, Bahia, 40170-115, Brazil

    Nubia Seyffert

  3. Laboratory of Cellular and Molecular Genetics, Biological Sciences Institute, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil

    Thiago Jesus Sousa & Vasco Azevedo

  4. National Reference Laboratory for Aquatic Animal Diseases, Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

    Henrique Cesar Pereira Figueiredo

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  1. Miriam Flores Rebouças
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  2. Dan Loureiro
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  9. Roberto Meyer
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Contributions

MFR, DL, NS, JTR, TJS, HCPF, RWP: experimental procedures, data preparation and interpretation, and writing the manuscript. TDB: experimental procedures, reviewing the manuscript. MFR, TDB, RWP: writing the manuscript. VA, RM: manuscript preparation, funding acquisition. All authors read and approved the final manuscript.

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Correspondence to Ricardo Wagner Portela.

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Rebouças, M.F., Loureiro, D., Barral, T.D. et al. Cell wall glycolipids from Corynebacterium pseudotuberculosis strains with different virulences differ in terms of composition and immune recognition. Braz J Microbiol 51, 2101–2110 (2020). https://doi.org/10.1007/s42770-020-00343-9

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