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Green synthesis of gold nanoparticles using extracellular metabolites of fish gut microbes and their antimicrobial properties

  • Biotechnology and Industrial Microbiology - Research Paper
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Abstract

In the present study, we synthesis nanoparticles using biosynthesis methods because of the eco-friendly approach. Gold nanoparticles were synthesized using extracellular metabolites of marine bacteria (Rastrelliger kanagurta, Selachimorpha sp., and Panna microdon). After the synthesis gold nanoparticles checked their antibacterial and antimycobacterial activities. Here we have few techniques that have been used for characterizing the gold nanoparticles followed by ultraviolet (UV)-visible spectrophotometer analysis, Fourier transform-infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM), and transmission electron microscopy (TEM). We observed the formation of gold nanoparticles using UV-Vis spectroscopy (UV-Vis). FT-IR spectroscopy results of the extracellular metabolites showed that different characteristic functional groups are responsible for the bioreduction of gold ions. In the recent years, we used zebrafish for an animal model to estimate nanoparticle toxicity and biocompatibility. We tested toxicity of the gold nanoparticle using the zebrafish larvae that are growing exponentially. Sample 1 showed a good antimicrobial activity, and sample 5 showed a good antimycobacterial activity. Based on the UV spectrophotometer, sample 1 is used for further studies. Color change and UV spectrum confirmed gold nanoparticles. Based on the TEM and SEM particles, size was measured and ranged between 80 and 45 nm, and most of the particles are spherical and are in rod shape. XRD result showed the gold nanoparticles with crystalline nature. Toxicity studies in the zebrafish larvae showed that 50 μg ml−1 showed less toxicity. Based on the studies, gold nanoparticle has good antibacterial and antimycobacterial activities. The present was concluded that gold nanoparticles have potential biocompatibility and less toxicity. Gold nanoparticles will be used as a drug molecule in pharmaceutical company and biomedicine application.

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Funding

The authors are grateful to the Science and Engineering Research Board (SERB-Early Career Research Award ECR/2015/000460), Government of India, New Delhi, India for the financial support and also thankful to the Management of Sathyabama Institute of Science and Technology (Deemed University) Chennai, Tamil Nadu, India, for providing the Infrastructure facility.

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Authors and Affiliations

  1. Centre for Drug Discovery and Development, Sathyabama Institute of Science and Technology (Deemed to be University) Jeppiaar Nagar, Rajiv Gandhi Road, Chennai, Tamil Nadu, 600 119, India

    T. Rajasekar, A. Maryshamya, S. Anbarasu & K. Revathy

  2. Departmentof Microbiology, Centre of Excellence in Life Sciences, Bharathidasan University, Tiruchirappalli, Tami Nadu, 620 024, India

    K. Karthika, G. Muralitharan & S. Sabarika

  3. P. G. & Research Departments of Microbiology, Sri Sankara Arts and Science College (Autonomous), Kanchipuram, Tamil Nadu, India

    N. Prasannabalaji

  4. Department of Biotechnology, Sathyabama Institute of Science and Technology (Deemed to be University) Jeppiaar Nagar, Rajiv Gandhi Road, Chennai, Tamil Nadu, 600 119, India

    S. Kumaran

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  1. T. Rajasekar
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  2. K. Karthika
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Correspondence to G. Muralitharan.

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Rajasekar, T., Karthika, K., Muralitharan, G. et al. Green synthesis of gold nanoparticles using extracellular metabolites of fish gut microbes and their antimicrobial properties. Braz J Microbiol 51, 957–967 (2020). https://doi.org/10.1007/s42770-020-00263-8

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  • DOI: https://doi.org/10.1007/s42770-020-00263-8

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