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Simultaneous hydrogen production and decolorization of denim textile wastewater: kinetics of decolorizing of indigo dye by bacterial and fungal strains

  • Environmental Microbiology - Research Paper
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Abstract

This study proposes the treatment and valorization of denim textile effluents through a fermentative hydrogen production process. Also, the study presents the decolorizing capabilities of bacterial and fungal isolates obtained from the fermented textile effluents. The maximum hydrogen production rate was 0.23 L H2/L-d, achieving at the same time color removal. A total of thirty-five bacteria and one fungal isolate were obtained from the fermented effluents and screened for their abilities to decolorize indigo dye, used as a model molecule. From them, isolates identified as Bacillus BT5, Bacillus BT9, Lactobacillus BT20, Lysinibacillus BT32, and Aspergillus H1T showed notable decolorizing capacities. Lactobacillus BT20 reached 90% of decolorization using glucose as co-substrate after 11 days of incubation producing colorless metabolites. Bacillus BT9 was able to utilize the indigo dye as the sole carbon source achieving a maximum decolorization of 60% after 9 days of incubation and producing a red-colored metabolite. In contrast, Bacillus BT5 and Lysinibacillus BT32 exhibited the lowest percentages of decolorization, barely 33% after 16 and 11 days of incubation, respectively. When Aspergillus H1T was grown in indigo dye supplemented with glucose, 96% of decolorization was reached after 2 days. This study demonstrates the valorization of denim textile effluents for the production of hydrogen via dark fermentation with concomitant color removal.

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Funding

This work was supported by the PROMEP/103.5/12/3680 and UGto-DAIP 000105/11. K.M. Muñoz-Páez acknowledges the support from CONACYT through the CÁTEDRAS program (Researcher ID 6407, Project 265).

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Author notes

  1. Idania Valdez-Vazquez

    Present address: Laboratory for Research on Advanced Processes for Water Treatment, Unidad Académica Juriquilla, Instituto de Ingeniería, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, 76230, Querétaro, Mexico

Authors and Affiliations

  1. DICIVA, Universidad de Guanajuato CIS, Ex Hacienda El Copal Km. 9, Carretera Irapuato-Silao, Irapuato, Guanajuato, Mexico

    Idania Valdez-Vazquez, Jhovana Gisela Robledo-Rizo, Marisol Pérez-Rangel & Graciela Ma. de la Luz Ruiz-Aguilar

  2. CONACYT - Instituto de Ingeniería, Unidad Académica Juriquilla, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, 76320 Querétaro, Mexico

    Karla María Muñoz-Páez

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  1. Idania Valdez-Vazquez
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  2. Jhovana Gisela Robledo-Rizo
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Correspondence to Idania Valdez-Vazquez.

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Highlights

- Hydrogen production and decolorization of denim effluents were achieved.

- Four bacteria and one fungus were isolated from the fermented effluent.

- Lactobacillus BT20 removed indigo dye completely using glucose as co-substrate.

- Bacillus BT9 used the dye as the sole carbon source and removed 60% of color.

- Aspergillus H1T decolorized indigo dye faster than the bacteria isolated.

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Valdez-Vazquez, I., Robledo-Rizo, J.G., Muñoz-Páez, K.M. et al. Simultaneous hydrogen production and decolorization of denim textile wastewater: kinetics of decolorizing of indigo dye by bacterial and fungal strains. Braz J Microbiol 51, 701–709 (2020). https://doi.org/10.1007/s42770-019-00157-4

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  • DOI: https://doi.org/10.1007/s42770-019-00157-4

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