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Batch and fed-batch production of probiotic biomass and nisin in nutrient-supplemented whey media

  • Biotechnology and Industrial Microbiology – Research Paper
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Abstract

The production of a highly concentrated probiotic preparation of Lactococcus lactis CECT 539 was studied in both batch and realkalized fed-batch fermentations in diluted whey (DW) media (DW25, DW50, DW75, DW100, and DW125) supplemented with MRS broth nutrients (except glucose and Tween 80) at 25, 50, 75, 100, and 125% of their standard concentrations in the complex medium. The fed-batch culture using DW100 medium provided the highest concentrations of probiotic biomass (5.98 g/L) and nisin (258.47 BU/mL), which were obtained at lower production costs than those estimated for the fed-batch culture in DW medium. The batch and fed-batch cultures reduced the initial chemical oxygen demand (COD) of the media by 29.1–41.7% and 31.2–54.2%, respectively.

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Acknowledgments

This work was supported by the Cluster de Investigación y Transferencia Agroalimentaria del Campus del Auga (CITACA), University of Vigo, Spain.

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Authors and Affiliations

  1. Department of Analytical and Food Chemistry, Science Faculty, University of Vigo, Ourense Campus, As Lagoas s/n, 32004, Ourense, Spain

    Mónica Costas Malvido, Elisa Alonso González, Delicia L. Bazán Tantaleán & Nelson Pérez Guerra

  2. Department of Engineering, Materials, Structural Mechanics and Construction, Science Faculty, University of Vigo, Ourense Campus, As Lagoas s/n, 32004, Ourense, Spain

    Ricardo J. Bendaña Jácome

Authors
  1. Mónica Costas Malvido
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  2. Elisa Alonso González
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  3. Delicia L. Bazán Tantaleán
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  4. Ricardo J. Bendaña Jácome
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  5. Nelson Pérez Guerra
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Correspondence to Nelson Pérez Guerra.

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Responsible Editor: Rosane Freitas Schwan

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Highlights

• A concentrated probiotic culture was produced in nutrient-supplemented whey media.

• Probiotic biomass and nisin synthesis depended on nutrient availability.

• Fed-batch cultures reduced the chemical oxygen demand of the media by 31.0–54.2%.

• Fed-batch cultures provided an almost constant biomass and nisin cost in all media.

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Malvido, M.C., González, E.A., Bazán Tantaleán, D.L. et al. Batch and fed-batch production of probiotic biomass and nisin in nutrient-supplemented whey media. Braz J Microbiol 50, 915–925 (2019). https://doi.org/10.1007/s42770-019-00114-1

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  • DOI: https://doi.org/10.1007/s42770-019-00114-1

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