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Development of a real-time nucleic acid sequence–based amplification assay for the rapid detection of Salmonella spp. from food

  • Food Microbiology - Research Paper
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Abstract

Salmonella spp. is one of the most common foodborne infectious pathogen. This study aimed to develop a real-time nucleic acid sequence–based amplification (NASBA) assay for detecting Salmonella in foods. Primers and a molecular beacon targeting the Salmonella-specific xcd gene were designed for mRNA transcription, and 48 Salmonella and 18 non-Salmonella strains were examined. The assay showed a high specificity and low detection limit for Salmonella (7 × 10−1 CFU/mL) after 12 h of pre-enrichment. Importantly, it could detect viable cells. Additionally, the efficacy of the NASBA assay was examined in the presence of pork background microbiota; it could detect Salmonella cells at 9.5 × 103 CFU/mL. Lastly, it was successfully used to detect Salmonella in pork, beef, and milk, and its detection limit was as low as 10 CFU/25 g (mL). The real-time NASBA assay developed in this study may be useful for rapid, specific, and sensitive detection of Salmonella in food of animal origin.

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Funding

This work was supported by grants from the National Science and Technology Support Program, the Social Development Program of Jiangsu Province, the Independent Innovation Program of Jiangsu Province, and the University Natura Science Key Project of Anhui Province (Grant Nos. 2012BAK08807, BE2012746, CX (12)3087, and KJ2016A182).

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Author notes

  1. Ligong Zhai

    Present address: College of Food and Drug, Anhui Science and Technology University, Fengyang, 233100, People’s Republic of China

Authors and Affiliations

  1. College of Food Science and Technology, Key Laboratory of Food Processing and Quality Control, Ministry of Agriculture of China, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Ligong Zhai, Hongxia Liu, Qiming Chen, Zhaoxin Lu, Chong Zhang, Fengxia Lv & Xiaomei Bie

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  1. Ligong Zhai
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  2. Hongxia Liu
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Correspondence to Xiaomei Bie.

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Zhai, L., Liu, H., Chen, Q. et al. Development of a real-time nucleic acid sequence–based amplification assay for the rapid detection of Salmonella spp. from food. Braz J Microbiol 50, 255–261 (2019). https://doi.org/10.1007/s42770-018-0002-9

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  • DOI: https://doi.org/10.1007/s42770-018-0002-9

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