Skip to main content
SpringerLink
Log in

Stability of insecticidal molecule aucubin and their toxicity on Anopheles stephensi, Aedes aegypti, Culex quinquefasciatus and Artemia salina

  • Original Research Article
  • Published:
International Journal of Tropical Insect Science Aims and scope Submit manuscript

Abstract

In the present study, aucubin molecule stability was tested in two different temperatures for 30 days. After incubation, the molecule toxicity was tested on Anopheles stephensi, Aedes aegypti, Culex quinquefasciatus and non-target organism Artemia salina and understands the aucubin molecule flexibility and intermolecular interaction mechanism with Acetylcholinesterase (AChE) and Odorant Binding Protein (OBP).After incubation, the aucubin molecules showed vigorous insecticidal activity against three mosquitoes as well as lower toxicity effects were observed on non-target organisms after 24 h post-treatment under laboratory conditions. Docking results suggested that aucubin has highly interacted with the Odorant Binding Protein (OBP) and Acetylcholinesterase (AChE) proteins. Further, aucubin is stable for 30 days and does not lose its efficacy in different thermal conditions, and is also cheaper, lower toxicity to non-target organisms and alternative synthetic chemical insecticides.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Abbott WS (1925) A method for computing the effectiveness of an insecticide. J Econ Entomol 18:265–267

    Article  CAS  Google Scholar 

  • Alkhaibari AM, Carolino AT, Bull JC, Samuels RI, Butt TM (2017) Differential pathogenicity of Metarhizium blastospores and conidia against larvae of three mosquito species. J Med Entomol 54:696–704

    Article  PubMed  CAS  Google Scholar 

  • Amiri-Besheli B, Khambay B, Cameron S, Deadman ML, Butt TM (2000) Inter-and intra-specific variation in destruction production by insect pathogenic Metarhizium spp., and its significance to pathogenesis. Mycol Res 104:447–452

    Article  CAS  Google Scholar 

  • Annapoorani CA, Manimegalai K (2013) Screening of medicinal plant Momordica charantia leaf for secondary metabolites. Int J Pharm Res Deve 5:1–6

    Google Scholar 

  • Baatrup ERIK, Bayley MARK (1993) Effects of the pyrethroid insecticide cypermethrin on the locomotor activity of the wolf spider Pardosa amentata: quantitative analysis employing computer-automated video tracking. Ecotoxicol Env Safe 26:138–152

    Article  CAS  Google Scholar 

  • Baleta A (2009) Insecticide resistance threatens malaria control in Africa. The Lancet 374:1581–1582

    Article  Google Scholar 

  • Benelli G, Duggan MF (2018) Management of arthropod vector data–social and ecological dynamics facing the One Health perspective. Act Trop 182:80–91

    Article  Google Scholar 

  • Brooijmans N, Kuntz ID (2003) Molecular recognition and docking algorithms. Ann Rev Biophys Biomol Struct 32:335–373

    Article  CAS  Google Scholar 

  • Charoy C, Janssen CR (1999) The swimming behavior of Brachionus calyciflorus (rotifer) under toxic stress: II. Comparative sensitivity of various behavioral criteria. Chemos 38:3247–3260

    Article  CAS  Google Scholar 

  • Charoy CP, Janssen CR, Persoone G, Clement P (1995) The swimming behavior of Brachionus calyciflorus (rotifer) under toxic stress. I. The use of automated trajectometry for determining sublethal effects of chemicals. Aqua Toxicol 32:271–282

    Article  CAS  Google Scholar 

  • Dogan YO, Hazir S, Yildiz A, Butt TM, Cakmak I (2017) Evaluation of entomopathogenic fungi for the control of Tetranychus urticae (Acari: Tetranychidae) and the effect of Metarhizium brunneum on the predatory mites (Acari: Phytoseiidae). Biol Control 111:6–12

    Article  Google Scholar 

  • Dowell D, Haegerich TM, Chou R (2016) CDC guideline for prescribing opioids for chronic pain—United States, 2016. JAMA 315(15):1624–1645

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Dumas C, Robert PH, Pais M, Vey A, Quiot JM (1994) Insecticidal and cytotoxic effects of natural and semisynthetic destruxins. Comp Biochem Physiol 108:195–203

    CAS  Google Scholar 

  • Gaddaguti V, Mounika SJ, Sowjanya K, Rao T, Chakravarthy MSRK, Allu R (2012) GC-MS analysis and in silico molecular docking studies of mosquito repellent compounds from Hyptis suaveolens. Int J Biomass 1:36–41

    Google Scholar 

  • Matowo J, Kulkarni MA, Mosha FW, Oxborough RM, Kitau JA, Tenu F, Rowland M (2010) Biochemical basis of permethrin resistance in A. arabiensis from Lower Moshi, North-eastern Tanzania. Malaria J 9:193

  • N’Guessan R, Boko P, Odjo A, Knols B, Akogbeto M, Rowland M (2009) Control of pyrethroid-resistant Anopheles gambiae and Culex quinquefasciatus mosquitoes with chlorfenapyr in Benin. Trop Med Int Heal 14:389–395

    Article  Google Scholar 

  • Nguta JM, Mbaria JM, Gakuya DW, Gathumbi PK, Kabasa JD, Kiama SG (2012) Evaluation of acute toxicity of crude plant extracts from Kenyan biodiversity using brine shrimp, artemia salina (artemiidae). The Open Conf Proc J 3:1

    Article  Google Scholar 

  • Pagadala NS, Syed K, Tuszynski J (2017) Software for molecular docking: a review. Biophys Rev 9(2):91–102

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Skrobek A, Butt TM (2005) Toxicity testing of destruxins and crude extracts from the insect-pathogenic fungus Metarhizium anisopliae. Microbiol Lett 25:23–28

    Article  Google Scholar 

  • Soonwera M, Phasomkusolsil S (2016) Effect of Cymbopogon citratus (lemongrass) and Syzygium aromaticum (clove) oils on the morphology and mortality of Aedes aegypti and Anopheles dirus larvae. Parasitol Res 115:1691–1703

    Article  PubMed  Google Scholar 

  • Suzuki A, Taguchi H, Tamura S (1970) Isolation and structure elucidation of three new insecticidal cyclodepsipeptides, destruxins C and D and desmethyldestruxin B, produced by Metarhizium anisopliae. Agric Biol Chem 34:813–816

    Article  CAS  Google Scholar 

  • Vivekanandhan P, Arunthirumeni M, Vengateswari G, Shivakumar MS (2018a) 5 Bioprospecting of novel fungal secondary metabolites for mosquito control. Microb Control Vector-Borne Dis

  • Vivekanandhan P, Kavitha T, Karthi S, Senthil-Nathan S, Shivakumar M (2018b) Toxicity of Beauveria bassiana-28 mycelial extracts on larvae of Culex quinquefasciatus mosquito (Diptera: Culicidae). Int J Env Res Pub Heal 15:440

    Article  Google Scholar 

  • Vivekanandhan P, Bedini S, Shivakumar MS (2020a) Isolation and identification of entomopathogenic fungus from Eastern Ghats of South Indian forest soil and their efficacy as biopesticide for mosquito control. J Parasitol Int 76:102099

    Article  CAS  Google Scholar 

  • Vivekanandhan P, Swathy K, Kalaimurugan D, Ramachandran M, Yuvaraj A, Kumar AN, Manikandan AT, Poovarasan N, Shivakumar MS, Kweka EJ (2020b) Larvicidal toxicity of Metarhizium anisopliae metabolites against three mosquito species and non-targeting organisms. PLoS One 15(5):e0232172. https://doi.org/10.1371/journal.pone.0232172

  • Vivekanandhan P, Swathy K, Thomas A, Kweka EJ, Rahman A, Pittarate S, Krutmuang P (2021) Insecticidal efficacy of microbial-mediated synthesized copper nano-pesticide against insect pests and non-target organisms. Int J Environ Res Public Health 18(19):10536

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • World Health Organization (WHO) (2005) Report of the Eighth WHOPES Working Group Meeting. Geneva. Guidelines for laboratory and field testing mosquito larvicides (WHO/CDS/ WHOPES/GCDPP/2005.13). Geneva, Switzerland

  • Zimmermann G (2007) Review on safety of the entomopathogenic fungus Metarhizium anisopliae. Biocon Sci Technol 17:879–920

    Article  Google Scholar 

Download references

Acknowledgements

P. Vivekanandhan thanks for providing financial supports under University Research Fellowship Scheme (Ref No. PU/AD-3/URF/2016) to Periyar University. We also thank the Institute of Vector Control and Zoonoses (IVCZ) Hosur for supplying eggs. We also express our thanks to the Department of Biotechnology, Periyar University, Salem, for successfully providing the infrastructural facility for carrying out our research.

Author information

Authors and Affiliations

  1. Molecular Entomology Laboratory, Department of Biotechnology, Periyar University, Salem, 636 011, Tamil Nadu, India

    P. Vivekanandhan, K. Swathy & M. S. Shivakumar

  2. Society for Research and Initiatives for Sustainable Technologies and Institutions, Grambharti, Amarapur, Gandhinagar, 382650, Gujarat, India

    P. Vivekanandhan & K. Swathy

Authors
  1. P. Vivekanandhan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Swathy
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. S. Shivakumar
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

P. Vivekanandhan designed the research; P. Vivekanandhan done the experiments and done the docking studies, interpreted the data, and P. Vivekanandhan wrote the manuscript, M. S. Shivakumar, K. Swathy and P. Vivekanandhan revised and approved the final draft.

Corresponding author

Correspondence to M. S. Shivakumar.

Ethics declarations

Ethics approval

The present research does not have any evaluation/test in humans performed by any of the authors in this article. We followed all national, international guidelines for the use of target and non-target organisms were followed.

Conflicts of interest

The authors declare that they do not have any conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOC 146 KB)

Rights and permissions

Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Vivekanandhan, P., Swathy, K. & Shivakumar, M.S. Stability of insecticidal molecule aucubin and their toxicity on Anopheles stephensi, Aedes aegypti, Culex quinquefasciatus and Artemia salina. Int J Trop Insect Sci 42, 3403–3417 (2022). https://doi.org/10.1007/s42690-022-00849-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s42690-022-00849-9

Keywords

Search

Navigation