Skip to main content
SpringerLink
Log in

The variation of reactive oxygen species scavenging enzymes and related gene expressions during occurrence and recovery of rubber tree tapping panel dryness

  • Original Paper
  • Published:
Journal of Rubber Research Aims and scope Submit manuscript

Abstract

Tapping panel dryness (TPD) in rubber tree (Hevea brasiliensis) is a complex physiological disorder which causes a serious loss of natural rubber yield. Many studies have been done to uncover the mechanism of TPD occurrence, but the mechanism remains unclear. Previous reports suggested that TPD occurrence might result from the over-generation of reactive oxygen species (ROS). To clarify the roles of ROS scavenging system in TPD response, rubber trees were stimulated by ethephon to induce TPD, and the resultant TPD trees were treated with 1-methylcyclopropene to induce their recovery. The latex samples were collected during the TPD occurrence and recovery to analyze the enzyme activities of catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione peroxidase (GPX) and peroxidase (POD), and the transcript expression of corresponding genes including HbCAT, HbCuZnSOD, HbAPX, HbGPX and HbPOD. The results showed that the activities of these ROS scavenging enzymes and expressions of the related genes got changed during the TPD occurrence and recovery, which suggests that the ROS scavenging genes and their corresponding enzymes were associated with TPD occurrence and recovery. Furthermore, these genes were also regulated by hydrogen peroxide (H2O2) and various hormone treatments, such as methyl jasmonate, salicylic acid, abscisic acid and so on. All these results suggest that the ROS scavenging genes and their corresponding enzymes are involved in responses to TPD, H2O2 and different hormones in rubber tree.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Zhu J, Zhang Z (2009) Ethylene stimulation of latex production in Hevea brasiliensis. Plant Signal Behav 4:1072–1074

    Article  CAS  Google Scholar 

  2. Yusof F, Siti Arija MA, Ghandimathi H, Hamzah Z, Sivakumaran S, Yeang HY (1995) Changes in some physiological latex parameters in relation to over exploitation and the onset of induced tapping panel dryness. J Nat Rubb Res 10:182–198

    CAS  Google Scholar 

  3. Dauzac J, Jacob JL, Chrestin H (1989) Biochical aspects of bark dryness induced by over-stimulation of rubber trees with ethrel. In: Chrestin H (ed) Physiology of rubber tree latex. CRC Press, Florida, pp 431–441

    Google Scholar 

  4. Li D, Deng Z, Chen C, Xia Z, Wu M, He P, Chen S (2010) Identification and characterization of genes associated with tapping panel dryness from Hevea brasiliensis latex using suppression subtractive hybridization. BMC Plant Biol 10:140

    Article  CAS  Google Scholar 

  5. Zhang Y, Leclercq J, Montoro P (2017) Reactive oxygen species in Hevea brasiliensis latex and relevance to tapping panel dryness. Tree Physiol 37:261–269

    Article  CAS  Google Scholar 

  6. Li D, Wu S, Dai L (2020) The rubber tree genome. In: Matsui M, Chow KS (eds) Current progress in transcriptomics and proteomics of latex physiology and metabolism in the Hevea brasiliensis rubber tree. Springer Press, Hoboken, pp 127–129

    Google Scholar 

  7. Das G, Raj S, Pothen J, Sethuraj MR, Sen-Mandi S (1998) Status of free radical and its scavenging system with stimulation in Hevea brasiliensis. Plant Physiol Bioch 25:47–50

    Google Scholar 

  8. Wang X, Wang D, Sun Y, Yang Q, Chang L, Wang L, Meng X, Huang Q, Jin X, Tong Z (2015) Comprehensive proteomics analysis of laticifer latex reveals new insights into ethylene stimulation of natural rubber production. Sci Rep 5:13778

    Article  Google Scholar 

  9. Yuan K, Zhou X, Wang Z, Yang L (2014) Identification and analysis of latex rubber particle proteins related with tapping panel dryness in Hevea brasiliensis. J Nanjing Forestry Univ (Natural Sci Ed) 38:36–40

    CAS  Google Scholar 

  10. Venkatachalam P, Thulaseedharan A, Raghothama KG (2007) Identification of expression profiles of tapping panel dryness (TPD) associated genes from the latex of rubber tree (Hevea brasiliensis Muell. Arg.). Planta 226:499–515

    Article  CAS  Google Scholar 

  11. Deng Z, Liu H, Wang YK, Li DJ (2014) Molecular cloning and expression analysis of a cytosolic glutathione reductase gene from Hevea brasiliensis. Zhiwu Shengli Xuebao 50:1699–1706

    CAS  Google Scholar 

  12. Deng Z, Zhao M, Liu H, Wang Y, Li D (2015) Molecular cloning, expression profiles and characterization of a glutathione reductase in Hevea brasiliensis. Plant Physiol Bioch 96:53–63

    Article  CAS  Google Scholar 

  13. Putranto RA, Herlinawati E, Rio M, Leclercq J, Piyatrakul P, Gohet E, Sanier C, Oktavia F, Pirrello J, Kuswanhadi MP (2015) Involvement of ethylene in the latex metabolism and tapping panel dryness of Hevea brasiliensis. Int J Mol Sci 16:17885–17908

    Article  CAS  Google Scholar 

  14. Yuan K, Wang Z, Zhou X, Zou Z, Yang L (2014) The identification of differentially expressed latex proteins in healthy and tapping panel dryness (TPD) Hevea brasiliensis trees by iTRAQ and 2D LC–MS/MS. Acta Agric Univ Jiangxiensis 36:650–655

    CAS  Google Scholar 

  15. Yuan K, Guo X, Feng C, Hu Y, Liu J, Wang Z (2019) Identification and analysis of a CPYC-type glutaredoxin associated with stress response in rubber trees. Forests 10:158

    Article  Google Scholar 

  16. Hu YY, Bai XQ, Feng CT, Yuan K, Liu H, Wang ZH (2020) Application of 1-MCP to tapping panel dryness of Hevea brasiliensis trees. J Northwest Forestry Univ 35:161–165

    Google Scholar 

  17. Tang C, Qi J, Li H, Zhang C, Wang Y (2007) A convenient and efficient protocol for isolating high-quality RNA from latex of Hevea brasiliensis (para rubber tree). J Bioch Bioph Meth 70:749–754

    Article  CAS  Google Scholar 

  18. Long X, He B, Wang C, Fang Y, Qi J, Tang C (2015) Molecular identification and characterization of the pyruvate decarboxylase gene family associated with latex regeneration and stress response in rubber tree. Plant Physiol Bioch 87:35–44

    Article  CAS  Google Scholar 

  19. Prasad TK, Anderson MD, Martin BA, Stewart CR (1994) Evidence for chilling-induced oxidative stress in maize seedlings and a regulatory role for hydrogen peroxide. Plant Cell 6:65–74

    Article  CAS  Google Scholar 

  20. Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399

    Article  CAS  Google Scholar 

  21. Camejo D, Guzmán-CedeñoÁ MA (2016) Reactive oxygen species, essential molecules, during plant-pathogen interactions. Plant Physiol Bioch 103:10–23

    Article  CAS  Google Scholar 

  22. Choudhury FK, Rivero RM, Blumwald E, Mittler R (2017) Reactive oxygen species, abiotic stress and stress combination. Plant J 90:856–867

    Article  CAS  Google Scholar 

  23. Segal LM, Wilson RA (2018) Reactive oxygen species metabolism and plant-fungal interactions. Fungal Genet Biol 110:1–9

    Article  CAS  Google Scholar 

  24. Mhamdi A, Van Breusegem F (2018) Reactive oxygen species in plant development. Development 145:15

    Article  CAS  Google Scholar 

  25. Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Bioch 48:909–930

    Article  CAS  Google Scholar 

  26. Choudhury S, Panda P, Sahoo L, Panda SK (2013) Reactive oxygen species signaling in plants under abiotic stress. Plant Signal Behav 8:e23681

    Article  CAS  Google Scholar 

  27. Hiraga S, Sasaki K, Ito H, Ohashi Y, Matsui H (2001) A large family of class III plant peroxidases. Plant Cell Physiol 42:462–468

    Article  CAS  Google Scholar 

  28. Wally O, Punja ZK (2010) Enhanced disease resistance in transgenic carrot (Daucus carota L.) plants over-expressing a rice cationic peroxidase. Planta 232:1229–1239

    Article  CAS  Google Scholar 

  29. Quiroga M, Guerrero C, Botella MA, Barcelo A, Amaya I, Medina MI, Alonso FJ (2000) A tomato peroxidase involved in the synthesis of lignin and suberin. Plant Physiol 122:1119–1127

    Article  CAS  Google Scholar 

  30. Kawaoka A, Matsunaga E, Endo S, Kondo S, Yoshida K, Shinmyo A, Ebinuma H (2003) Ectopic expression of a horseradish peroxidase enhances growth rate and increases oxidative stress resistance in hybrid aspen. Plant Physiol 132:1177–1185

    Article  CAS  Google Scholar 

  31. Passardi F, Cosio C, Penel C, Dunand C (2005) Peroxidases have more functions than a Swiss army knife. Plant Cell Rep 24:255–265

    Article  CAS  Google Scholar 

  32. Bindschedler LV, Dewdney J, Blee KA, Stone JM, Asai T, Plotnikov J, Denoux C, Hayes T, Gerrish C, Davies DR, Ausubel FM, Bolwell GP (2006) Peroxidase-dependent apoplastic oxidative burst in Arabidopsis required for pathogen resistance. Plant J 47:851–863

    Article  CAS  Google Scholar 

  33. Wang LF (2014) Physiological and molecular responses to drought stress in rubber tree (Hevea brasiliensis Muell. Arg.). Plant Physiol Bioch 83:243–249

    Article  CAS  Google Scholar 

  34. Wang LF (2014) Physiological and molecular responses to variation of light intensity in rubber tree (Hevea brasiliensis Muell. Arg.). PLoS ONE 9:e89514

    Article  CAS  Google Scholar 

  35. Zhang Y, Leclercq J, Wu S, Ortega-Abboud E, Pointet S, Tang C, Hu S, Montoro P (2019) Genome-wide analysis in Hevea brasiliensis laticifers revealed species-specific post-transcriptional regulations of several redox-related genes. Sci Rep 9:5701

    Article  CAS  Google Scholar 

  36. Montoro P, Wu S, Favreau B, Herlinawati E, Labrune C, Martin-Magniette ML, Pointet S, Rio M, Leclercq J, IsmawantoKuswanhadi SD (2018) Transcriptome analysis in Hevea brasiliensis latex revealed changes in hormone signalling pathways during ethephon stimulation and consequent Tapping Panel Dryness. Sci Rep 8:8483

    Article  CAS  Google Scholar 

  37. Leclercq J, Martin F, Sanier C, Clement-Vidal A, Fabre D, Oliver G, Lardet L, Ayar A, Peyramard M, Montoro P (2012) Overexpression of a cytosolic isoform of the HbCuZnSOD gene in Hevea brasiliensis changes its response to a water deficit. Plant Mol Biol 80:255–272

    Article  CAS  Google Scholar 

  38. Chao J, Zhang S, Chen Y, Tian WM (2015) Cloning, heterologous expression and characterization of ascorbate peroxidase (APX) gene in laticifer cells of rubber tree (Hevea brasiliensis Muell. Arg.). Plant Physiol Bioch 97:331–338

    Article  CAS  Google Scholar 

  39. Wang LF, Wang JK, An F, Xie GS (2016) Molecular cloning and characterization of a stress responsive peroxidase gene HbPRX42 from rubber tree. Braz J Bot 39:475–483

    Article  Google Scholar 

  40. Liu ZJ, Guo YK, Bai JG (2010) Exogenous hydrogen peroxide changes antioxidant enzyme activity and protects ultrastructure in leaves of two cucumber ecotypes under osmotic stress. J Plant Growth Regul 29:171–183

    Article  CAS  Google Scholar 

  41. Li JT, Qiu ZB, Zhang XW, Wang LS (2011) Exogenous hydrogen peroxide can enhance tolerance of wheat seedlings to salt stress. Acta Physiol Plant 33:835–842

    Article  CAS  Google Scholar 

  42. Neill S, Desikan R, Hancock J (2002) Hydrogen peroxide signalling. Curr Opin Plant Biol 5:388–395

    Article  CAS  Google Scholar 

  43. Polidoros AN, Scandalios JG (1999) Role of hydrogen peroxide and different classes of antioxidants in the regulation of catalase and glutathione S-transferase gene expression in maize (Zea mays L.). Physiol Plantarum 106:112–120

    Article  CAS  Google Scholar 

  44. Almeida JM, Fidalgo F, Confraria A, Santos A, Pires H, Santos I (2005) Effect of hydrogen peroxide on catalase gene expression, isoform activities and levels in leaves of potato sprayed with homobrassinolide and ultrastructural changes in mesophyll cells. Funct Plant Biol 32:707–720

    Article  CAS  Google Scholar 

  45. Morita S, Kaminaka H, Masumura T, Tanaka K (1999) Induction of rice cytosolic ascorbate peroxidase mRNA by oxidative stress; the involvement of hydrogen peroxide in oxidative stress signalling. Plant Cell Physiol 40:417–422

    Article  CAS  Google Scholar 

  46. Tsai YC, Hong CY, Liu LF, Kao CH (2005) Expression of ascorbate peroxidase and glutathione reductase in roots of rice seedlings in response to NaCl and H2O2. J Plant Physiol 162:291–299

    Article  CAS  Google Scholar 

  47. Qin YM, Hu CY, Zhu YX (2008) The ascorbate peroxidase regulated by H2O2 and ethylene is involved in cotton fiber cell elongation by modulating ROS homeostasis. Plant Signal Behav 3(3):194–196

    Article  Google Scholar 

  48. Levine A, Tenhaken R, Dixon R, Lamb C (1994) H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response. Cell 79:583–593

    Article  CAS  Google Scholar 

  49. Chen HJ, Wu SD, Huang GJ, Shen CY, Afiyanti M, Li WJ, Linc YH (2012) Expression of a cloned sweet potato catalase SPCAT1 alleviates ethephon-mediated leaf senescence and H2O2 elevation. J Plant Physiol 169:86–97

    Article  CAS  Google Scholar 

  50. Agrawal GK, Jwac NS, Iwahashid H, Rakwal R (2003) Importance of ascorbate peroxidases OsAPX1 and OsAPX2 in the rice pathogen response pathways and growth and reproduction revealed by their transcriptional profiling. Gene 322:93–103

    Article  CAS  Google Scholar 

  51. Hao BZ, Wu JL (2000) Laticifer differentiation in Hevea brasiliensis: induction by exogenous jasmonic acid and linolenic acid. Ann Bot 85:37–43

    Article  CAS  Google Scholar 

  52. Zeng RZ, Duan CF, Li XY, Tian WM, Nie ZY (2009) Vacuolar-type inorganic pyrophosphatase located on the rubber particle in the latex is an essential enzyme in regulation of the rubber biosynthesis in Hevea brasiliensis. Plant Sci 176:602–607

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We would like to thank Prof. Jiannan Zhou for his revision of the English.

Funding

This research was funded by the Ministry of Agriculture and Rural Affairs of People’s Republic of China (1630022020010) and the China Agriculture Research System-Natural Rubber (CARS-33-ZP1).

Author information

Authors and Affiliations

  1. Rubber Research Institute, Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Genetic Resources of Rubber Tree, Chinese Academy of Tropical Agricultural Sciences, No.4 Xueyuan Road, Haikou, 571101, Hainan, China

    Kun Yuan, Yiyu Hu, Chengtian Feng & Zhenhui Wang

  2. Institute of Tropical Agriculture and Forestry, Hainan University, No.58 Renmin Road, Haikou, 570228, Hainan, China

    Jing He

Authors
  1. Kun Yuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jing He
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yiyu Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chengtian Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zhenhui Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhenhui Wang.

Ethics declarations

Conflicts of interest

The authors declare no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yuan, K., He, J., Hu, Y. et al. The variation of reactive oxygen species scavenging enzymes and related gene expressions during occurrence and recovery of rubber tree tapping panel dryness. J Rubber Res 24, 391–402 (2021). https://doi.org/10.1007/s42464-021-00106-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s42464-021-00106-7

Keywords

Search

Navigation