Skip to main content
SpringerLink
Log in

Characterization of 16SrII group phytoplasma associated with sesame phyllody disease in different cropping seasons

  • Short Communication
  • Published:
Indian Phytopathology Aims and scope Submit manuscript

Abstract

A survey was conducted in the sesame fields of Karnataka, India during April–June 2010 (summer) and in Telangana State, Maharashtra and Karnataka during July–September 2010 (Kharif). In summer, disease incidence of 18.57–31.6% was recorded, whereas, in Kharif, incidence of 44.66–68%, 13–47.14% and 32.14% was recorded in Telangana State, Karnataka and Maharashtra respectively. Three symptomatic and two non-symptomatic samples from summer crop and sixteen symptomatic and two non symptomatic samples from Kharif season fields were collected. The disease etiology was confirmed using nested polymerase chain reaction (PCR) with phytoplasma universal primers P1/P7 followed by R16F2n/R16R2 which amplified ~ 1.8 and ~ 1.25 kb in the 16SrDNA gene. The BLAST and phylogenetic analysis of 16S rDNA gene further revealed that phytoplasma associated with phyllody disease in both the season were belonged to peanut witches’ broom group (16SrII). Further, iPhyClassifier analysis of 1.25 kb product of 16S rDNA sequence classified the isolates into 16SrII-A and 16SrII-D subgroups. All summer crop isolates were classified under 16SrII-D subgroup whereas; Kharif season isolates were grouped under 16SrII-A and 16SrII-D group. Phylogenetic analysis revealed the distinct subgroup-wise as well as season-wise clustering pattern where subgroup 16SrII-A and 16SrII-D clustered separately and summer and Kharif season isolates clustered separately. Our study reports that the sesame phyllody disease in Kharif season is caused by the taxonomically distinct strains (16SrII-A and 16SrII-D) whereas, in the summer crop, it is associated with 16SrII-D subgroup alone.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  • Abraham EV, Natarajan K, Murugaesan M (1977) Damage by pests and phyllody to Sesamum indicum in relation to time sowing. Madras Agric J 64:298–301

    Google Scholar 

  • Ahrens U, Seemüller E (1992) Detection of DNA of plant pathogenic mycoplasma-like organisms by a polymerase chain reaction that amplifies a sequence of the 16SrRNA gene. Phytopathol 82:828–832

    Article  CAS  Google Scholar 

  • Akhtar KP, Dickinson M, Sarwar G, Jamil FF, Haq MA (2008) First report on the association of a 16SrII phytoplasma with sesame phyllody in Pakistan. Plant Pathol 57:771

    Google Scholar 

  • Al Sakeiti MA, Al-Subhi AM, Saady NA, Deadman ML (2005) First report of witches’ broom disease of sesame (Sesamum indicum) in Oman. Plant Dis 89:530

    Article  CAS  Google Scholar 

  • Catal M, Ikten C, Yol E, Ustun R, Uzun B (2013) First report of 16SrIX group phytoplasma associated with sesame phyllody in Turkey. Plant Dis 97:835

    Article  CAS  Google Scholar 

  • Cengiz I, Mursel C, Engin Y, Rustem U, Seymus F, Cengiz T, Bulent U (2014) Molecular identification, characterization and transmission of phytoplasmas associated with sesame phyllody in Turkey. Eur J Plant Pathol 139:217–229

    Article  Google Scholar 

  • Deng S, Hiruki C (1991) Amplification of 16SrRNA genes from culturable and nonculturable mollicutes. J Microbiol Methods 14:53–61

    Article  CAS  Google Scholar 

  • FAOSTAT (2013) faostat3.fao.org/faostat-gateway/go/to/search/oilseeds/E

  • Gundersen DE, Lee IM (1996) Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathol Mediterr 35:144–151

    CAS  Google Scholar 

  • Khan MS, Raj SK, Snehi SK (2007) First report of molecular identification of ‘Candidatus Phytoplasma asteris’ affecting sesame (Sesamum indicum) cultivation in India. J Plant Pathol 89:301–305

    Google Scholar 

  • Kumar S, Singh V, Lakhanpal S (2011) Occurrence of spiroplasma and phytoplasma in sesame affected with yellowing disease in India. Phytopathogenic Mollicutes 1:47–49

    Article  Google Scholar 

  • Madhupriya, Rao GP, Kumar A, Baranwal VK (2015) Classification of sesame phytoplasma strain in India at 16Sr subgroup level. J Plant Pathol 3:523–528

    Google Scholar 

  • Maiti S, Hegde MR, Chattopadhyay SB (1988) Handbook of oil seed crops. Oxford and IBH Publishing Co. (Pvt.). Ltd, New Delhi, p 317

    Google Scholar 

  • Manjunatha N, Prameela HA, Rangaswamy KT, Palanna KB, Wickramaaracgchi W (2012) Phyllody phytoplasmal infecting sesame in South India. Phytopathogenic Mollicutes 2:29–32

    Article  Google Scholar 

  • Nabi S, Madhupriya, Dubey DK, Rao GP, Baranwal VK, Sharma P (2015a) Characterization of phytoplasmas associated with sesame (Sesamum indicum) phyllody disease in North India utilizing multilocus genes and RFLP analysis. Indian Phytopathol 68(1):112–119

    Google Scholar 

  • Nabi S, Madhupriya, Dubey DK, Rao GP, Baranwal VK, Sharma P (2015b) Molecular characterization of ‘Candidatus Phytoplasma asteris’ subgroup I–B associated with sesame phyllody disease and identification of its natural vector and weed reservoir in India. Australas Plant Pathol 44:289–297

    Article  Google Scholar 

  • Rao GP, Nabi SU, Madhupriya (2015) Overview on a century progress in research on sesame phyllody disease. Phytopathogenic Mollicutes 5(2):74–83

    Article  Google Scholar 

  • Sambrook J, Russel DW (2001) Molecular cloning; a laboratory manual, 2nd edn. Cold Spring Harbour Laboratory Press, Harbor

    Google Scholar 

  • Sarwar G, Haq MA (2006) Evaluation of sesame germplasm for genetic parameters and disease resistance. J Agric Res 44:89–96

    Google Scholar 

  • Sertkaya G, Martini M, Musetti R, Osler R (2007) Detection and molecular characterization of phytoplasmas infecting sesame and solanaceous crops in Turkey. Bull Insectol 60:141–142

    Google Scholar 

  • Singh V, Kumar S, Reddy BNS, Naik MK, Bhat KV, Lakhanpaul S (2016) Devastation of sesame (Sesamum indicum L.) crops in different agroclimatic zones of India by genetically diverse subgroups of phytoplasma. Crop Prot 86:24–30

    Article  CAS  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2013) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28(10):2731–2739

    Article  Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties, and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  CAS  Google Scholar 

  • Vasudeva RS, Sahambi HS (1955) Phyllody in sesamum (Sesamum orientale L.). Indian Phytopathol 8:124–129

    Google Scholar 

  • Win NKK, Back C, Jung HY (2010) Phyllody Phytoplasma infecting sesame in Myanmar belongs to group 16SrI and subgroup 16SrI-B. Tropical Plant Pathol 35:310–313

    Article  Google Scholar 

  • Zhao Y, Wei W, Lee IM, Shao J, Suo X, Davis RE (2009) Construction of an interactive online phytoplasma classification tool, iPhyClassifier, and its application in analysis of the peach X-disease phytoplasmal group (16SrIII). Int J Syst Evol Microbiol 59:2582-259

    Google Scholar 

Download references

Acknowledgement

Authors are grateful to Dean, Postgraduate studies, UAS, Raichur for providing necessary research facilities and are also thankful to the Director, ICAR-NBPGR, New Delhi for sharing laboratory facilities.

Author information

Authors and Affiliations

  1. Department of Plant Pathology, University of Agricultural Sciences, Raichur, 584 103, India

    Pramesh Devanna, M. K. Naik & Reddy Bharath

  2. ICAR-National Research Centre for DNA Finger Printing, New Delhi, 110 012, India

    K. V. Bhat

  3. ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India

    P. Madupriya

Authors
  1. Pramesh Devanna
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. K. Naik
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Reddy Bharath
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. V. Bhat
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P. Madupriya
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Pramesh Devanna.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Devanna, P., Naik, M.K., Bharath, R. et al. Characterization of 16SrII group phytoplasma associated with sesame phyllody disease in different cropping seasons. Indian Phytopathology 73, 563–568 (2020). https://doi.org/10.1007/s42360-020-00212-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s42360-020-00212-3

Keywords

Search

Navigation