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Bioactivity assessment of endophytic fungi associated with Citrus macroptera Montr.: an endangered ethnomedicinal plant used in folk medicines in North-East India

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Abstract

Thirteen endophytic fungal isolates, inhabiting Citrus macroptera growing in Assam, India, were studied for their ability to produce bioactive metabolites. Ethyl acetate extracted secondary metabolites of endophytic Talaromyces assiutensis, exhibited the highest antimicrobial activity against Staphylococcus epidermidis followed by Acremonium falciformae which inhibited Sclerotinia sclerotiorum. Taxonomic identity of T. assiutensis was confirmed by internal transcribed spacer r-DNA sequence analysis. Quantitative estimation of phytochemicals of the ethyl acetate extracted secondary metabolites produced by T. assiutensis, revealed that flavonoid content was the highest followed by alkaloids, saponin and terpenoids. FT-IR spectrophotometry of the extract showed the presence of alcohol, alkane, acid anhydride, alkene, aromatic, alkyl halide and amine as functional groups. Nine major cmpounds, viz., 3-undecene-5-methyl-; 2,5-dihydroxy-3-methyl-2,5-cyclohexadiene-1,4-dione; 3-n-hexylthiolane, S,S-dioxide; Methyl 8-Methylnonanoate; (4H)4a,5,6,7,8,8a-Hexahydrobenzopyran-5-one-3-carboxamide,2; Cyclopentane undecanoic acid, methyl ester; Trans-2,4-Dimethylthiane, S,S-dioxide; 5-Hydroxy-4-hydroxymethyl-1-(1-hydroxy-1-isopropyl)-Cyclohex-3-ene and 3-Nonyn-1-ol were identified from the bioactive metabolites of T. assiutensis using GC–MS. The results indicate that the antimicrobial compounds produced by T. assiutensis may be used in pharmaceutical as well as agricultural industries.

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References

  • Aktar K (2017) Foyzun T (2017) Phytochemistry and Pharmacological Studies of Citrus macroptera: a medicinal plant review. Evid Based Complem Altern Med 7:1–7. https://doi.org/10.1155/2017/9789802

    Article  Google Scholar 

  • Bauer AW, Kirby WMM, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493–496

    Article  CAS  Google Scholar 

  • Bhardwaj A, Agrawal PK (2015) A review fungal endophytes: as a store house of bioactive compound. World J Pharma Sci 3(9):228–237

    Google Scholar 

  • Carroll GC, Carroll FE (1978) Studies on the incidence of coniferous needle endophytes in the Pacific Northwest. Can J Bot 56(24):3034–3043. https://doi.org/10.1139/b78-367

    Article  Google Scholar 

  • Deka D, Jha DK (2018) Antimicrobial Activity of Endophytic Fungi from Leaves and Barks of Litsea cubeba Pers., a Traditionally Important Medicinal Plant of North East India. Jordan J Biol Sci 11(1):73–79

    CAS  Google Scholar 

  • Devi NN, Prabakaran JJ (2014) Bioactive metabolites from an endophytic fungus Penicillium sp. isolated from Centella asiatica. Curr Res Environ App Mycol 4(1):34–43

    Article  Google Scholar 

  • Dhankhar S, Dhankhar S, Kumar M, Ruhil S, Balhara M, Chillar AK (2012) Analysis toward innovative herbal antibacterial & antifungal drugs. Recent Pat Anti-Infect Drug Discovery 7(3):242–248

    Article  CAS  Google Scholar 

  • Dix NJ, Webster J (1995) Fungal ecology. University of Michigan, Chapman & Hall, London

    Book  Google Scholar 

  • Ferguson NM (1956) A text book of pharmacognosy. Mac Milan Company, New Delhi, pp 969–973

    Google Scholar 

  • Ginting RCB, Sukarno N, Widyastuti U, Darusman L, Kanaya S (2013) Diversity of Endophytic Fungi from Red Ginger (Zingiber officinale Rosc.) Plant and Their Inhibitory Effect to Fusarium oxysporum Plant Pathogenic Fungi. Hayati J Biosci 20(3):127-137. http://journal.ipb.ac.id/index.php/hayati

  • Hameed IH, Altameme HJ, Mohammed GJ (2016) Evaluation of antifungal and antibacterial activity and analysis of bioactive phytochemical compounds of Cinnamomum zeylanicum (cinnamon bark) using gas chromatography–mass spectrometry. Orient J Chem 32(4):1769–1788

    Article  CAS  Google Scholar 

  • Handunnetti SM, Kumara RR, Deraniyagala SA, Ratnasooriya WD (2009) Anti-inflammatory activity of Ixora coccinea methanolic leaf extract. Pharmacog Res 1(2):80–90

    Google Scholar 

  • Hata K, Futai K (1995) Endophytic fungi associated with healthy pine needles and needles infested by the pine needle gall midge, Thecodiplosis japonensis. Can J Bot 73(3):384–390

    Article  Google Scholar 

  • Huang WY, Cai YZ, Ling J, Corke H, Sun M (2007) A potential antioxidant resource: endophytic fungi from medicinal plants. Econ Bot 61(1):14–30

    Article  CAS  Google Scholar 

  • Kaaniche F, Hamed A, Abdel-Razek AS, WibbergD Abdissa N, El Euch IZ (2019) Bioactive secondary metabolites from new endophytic fungus Curvularia. sp isolated from Rauwolfia macrophylla. PLoS One 14(6):e0217627. https://doi.org/10.1371/journal.pone.0217627

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Keith LM, Velasquez ME, Zee FT (2006) Identification and characterization of Pestalotiopsis spp. Causing scab disease of Guava, Psidium guajava. Plant Dis 90:16–23

    Article  CAS  Google Scholar 

  • Kusari S, Verma VC, Lamshoft M, Spiteller M (2012) An endophytic fungus from Azadirachta indica A. Juss. that produces azadirachtin. World J Microbiol Biotechnol 28:1287–1294

    Article  CAS  Google Scholar 

  • Maheswari S, Rajagopal K (2013) Biodiversity of endophytic fungi in Kigelia pinnata during two different seasons. Curr Sci 104:515–518

    Google Scholar 

  • Malik SK, Chaudhury R, Dhariwal OP, Kalia RK (2006) Collection and Characterization of Citrus indica Tanaka and C. macroptera Montr.: wild endangered species of Northeastern India. Genet Resour Crop Evol 53(7):1485–1493. https://doi.org/10.1007/s10722-005-7468-7

    Article  Google Scholar 

  • Miao F, Yang R, Chen DD, Wang Y, Qin BF, Yang XJ, Zhou L (2012) Isolation, identification and antimicrobial activities of two secondary metabolites of Talaromyces verruculosus. Molecules 17(12):14091–14098. https://doi.org/10.3390/molecules171214091

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nagamani A, Kunwar SK, Monoharachary C (2006) Handbook of soil fungi. I. K. International Pvt. Ltd., New Delhi

    Google Scholar 

  • Nithyadevi J, Sivakumar R (2015) Phytochemical screening and GC–MS, FT-IR analysis of methanolic extract leaves of Solanum torvum Sw. Int J Res Biosc 3(9):61–66

    Google Scholar 

  • Pervez MR, Musaddiq M, Thakare PV, Kumar A (2015) Characterization of bioactive compound isolated from Myrothecium spp. with UV, FTIR and HPLC Analysis. Indian J Pharm Biol Res 3(1):1–5

    Article  CAS  Google Scholar 

  • Phongpaichit S, Nikom J, Rungjindamai N, Sakayaroj J, Hutadilok-Towatana N, Rukachaisirikul V, Kirtikara K (2007) Biological activities of extracts from endophytic fungi isolated from Garcinia plants. FEMS Immunol Med Microbiol 51:517–525

    Article  CAS  Google Scholar 

  • Prasad DA, Prasad BR, Prasad DK, Shetty P, Kumar KNS (2016) GC–MS Compositional analysis of essential oil of leaf and fruit rind of Citrus maxima (Burm.) Merr. from Coastal Karnataka, India. J App Pharm Sci 6(5):68–72

    Article  Google Scholar 

  • Salvatore MM, DellaGreca M, Nicoletti R, Salvatore F, Vinale F, Naviglio D, Andolfi A (2018) Talarodiolide, a new 12-membered macrodiolide, and gc/ms investigation of culture filtrate and mycelial extracts of Talaromyces pinophilus. Molecules 23:950–959

    Article  Google Scholar 

  • Santos H, Filho P (2003) Doenças de fruteiras tropicais de interesse agroindustrial. Embrapa Informacao Tecnologica Brasilia 11:435–491

    Google Scholar 

  • Schulz B, Wanke V, Draeger S, Aust HJ (1993) Endophytes from herbaceous plants and shrubs: effectiveness of surface sterilization methods. Mycol Res 97:1447–1450

    Article  Google Scholar 

  • Shamsa F, Hamidreza G, Monsef RG, Mohammadreza V (2008) Spectrophotometric determination of total alkaloids in some Iranian medicinal plants. Thai J Pharmaceutical Sci 32:17–20

    CAS  Google Scholar 

  • Sharma D, Pramanik A, Agrawal PK (2016) Evaluation of bioactive secondary metabolites from endophytic fungus Pestalotiopsis neglecta BAB-5510 isolated from leaves of Cupressus torulosa D. Don. 3 Biotech 6:2-10

  • Stierle A, Strobel GA, Stierle D (1993) Taxol and taxane production by Taxomyces andreanae. Science 260:214–216

    Article  CAS  Google Scholar 

  • Strobel G, Daisy B (2003) Bioprospecting for microbial endophytes and their natural products. Microbiol Mol Biol Rev 67(4):491–502

    Article  CAS  Google Scholar 

  • Strobel G, Ford E, Worapong J, Harper JK, Arif AM, Grant DM (2002) Isopestacin, a unique isobenzofuranone from Pestalotiopsis microspora possessing antifungal and antioxidant properties. Phytochem 60:179–183

    Article  CAS  Google Scholar 

  • Tayung K (2008) Studies on Endophytic Microorganisms Associated with Taxus sp. Ph.D. thesis, Gauhati University, Guwahati, Assam, India

  • Teiten MH, Gaascht F, Dicato M, Diederich M (2013) Anticancer bioactivity of compounds from medicinal plants used in European medieval traditions. Biochem Pharmacol 86(9):1239–1247. https://doi.org/10.1016/j.bcp.2013.08.007

    Article  CAS  PubMed  Google Scholar 

  • White TJ, Bruns TD, Lee SB, Taylor JW (1990a) Amplification and direct sequencing of fungal ribosomal RNA. Gen Phylogenet 38:315–322

    Google Scholar 

  • White TJ, Bruns TD, Lee SB, Taylor JW (1990b) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, New York, pp 315–322

    Google Scholar 

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Acknowledgement

Deepanwita Deka is thankful to the University Grant Commission, New Delhi for providing Basic Scientific Research (UGC-BSR) fellowship under Special Assistance Program (UGC-SAP, DRS-I), Department of Botany, Gauhati University, Guwahati.

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  1. Microbial Ecology Laboratory, Department of Botany, Gauhati University, Guwahati, Assam, 781014, India

    Deepanwita Deka & Dhruva Kumar Jha

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  1. Deepanwita Deka
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  2. Dhruva Kumar Jha
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Correspondence to Dhruva Kumar Jha.

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Deka, D., Jha, D.K. Bioactivity assessment of endophytic fungi associated with Citrus macroptera Montr.: an endangered ethnomedicinal plant used in folk medicines in North-East India. Indian Phytopathology 73, 21–33 (2020). https://doi.org/10.1007/s42360-019-00179-w

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