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Difficulties in identifying Xanthomonas citri subsp. citri A pathotypes

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Abstract

A total of 350 citrus canker bacterial isolates, representing different citrus growing regions in Saudi Arabia, were identified as Xanthomonas citri subsp. citri (Xcc). DNA alignment of the 16S-rDNA of the three citrus canker organisms (Xcc, pathotypes A, X. fuscans pv. aurantifolii, pathotype B, and C) showed that they can be distinguished by using two SNPs. All the 350 Saudi Xcc strains belonged to the 16-rDNA haplotype of Xcc-A pathotypes. Pathogenicity in leaf assays using differential citrus cultivars, grapefruit (Citrus paradisi) and Mexican lime (C. aurantifolia), divided the Saudi Arabian bacterial isolates into two groups. The first group produced typical symptoms of Xcc, including erumpent canker lesions with water-soaked margins on all hosts. However, the second group caused atypical symptoms on grapefruit leaves. REP-PCR analysis grouped Xcc strains into three clusters that were not correlated with host, pathogenicity or geographic origin. However, the Baha Xcc population had the highest genetic variability, suggesting that this region may be responsible for disseminating Xcc strains to Saudi southern citrus-growing regions through movement of contaminated planting materials. Although we were able to definitely identify the Saudi citrus canker bacteria as Xcc-A pathotypes, its pathotypes still needs more investigation to be recognized. To our knowledge, this also is the first report of occurrence of citrus canker of sweet orange in the Abha region of Saudi Arabia.

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Acknowledgements

The authors would like to extend their sincere appreciation to the Deanship of Scientific Research, King Saud University, Saudi Arabia, for its funding of this research group No. RG-1438-065.

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Authors and Affiliations

  1. Plant Protection Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, 11451, Riyadh, Kingdom of Saudi Arabia

    Yasser E. Ibrahim, Mahmoud H. El Komy, Mahmoud A. Amer, Arya Widyawan, Mohammed A. Al-Saleh & Amgad A. Saleh

  2. Agriculture Research Center, Plant Pathology Research Institute, Giza, Egypt

    Yasser E. Ibrahim, Mahmoud H. El Komy & Mahmoud A. Amer

  3. Agriculture Research Center, Agricultural Genetic Engineering Research Institute, Giza, Egypt

    Amgad A. Saleh

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  1. Yasser E. Ibrahim
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  2. Mahmoud H. El Komy
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  3. Mahmoud A. Amer
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  4. Arya Widyawan
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  5. Mohammed A. Al-Saleh
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  6. Amgad A. Saleh
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Correspondence to Yasser E. Ibrahim.

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Ibrahim, Y.E., El Komy, M.H., Amer, M.A. et al. Difficulties in identifying Xanthomonas citri subsp. citri A pathotypes. J Plant Pathol 101, 927–933 (2019). https://doi.org/10.1007/s42161-019-00294-7

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  • DOI: https://doi.org/10.1007/s42161-019-00294-7

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