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Pressurized Liquid Extraction of Pigments from Chlamydomonas sp. and Chemical Characterization by HPLC–MS/MS

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Abstract

Pressurized liquid extraction using green solvents (ethanol and water) was applied to extract photosynthetic pigments from the microalgae Chlamydomonas sp. An experimental design is employed to determine the effect of the extraction conditions on the extraction yield and the antioxidant capacity of the different extracts determined using 1,1-diphenyl-2-picryl-hydrazyl and TEAC tests. All the extracts show significant antioxidant activity in all assays. Besides, in this work, the carotenoid and chlorophyll composition of the Chlamydomonas extracts was analyzed by HPLC–DAD–APCI–MS/MS for the first time, being the main compounds lutein and pheophythin a´.

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Acknowledgements

This work was supported by EU MIRACLES project (Seventh Framework Programme KBBE- Grant Agreement No. 613588, Multi-product Integrated bioRefinery of Algae: from Carbon dioxide and Light Energy to high-value Specialties).

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Authors and Affiliations

  1. Applied Analytical Chemistry, University of Duisburg-Essen, Universitaetsstr. 5, 45141, Essen, Germany

    Lidia Montero

  2. Laboratory of Foodomics, Bioactivity and Food Analysis Department, Institute of Food Science Research CIAL (CSIC-UAM), Campus de Cantoblanco, C/Nicolás Cabrera 9, 28049, Madrid, Spain

    Mahshid Sedghi, Alejandro Cifuentes, Jose A. Mendiola & Elena Ibáñez

  3. Spanish Bank of Algae, Instituto de Oceanografía y Cambio Global, Fundación Canaria Parque Científico-Tecnológico, Universidad de Las Palmas de Gran Canaria, Muelle de Taliarte s/n, 35214, Telde (Canary Islands), Spain

    Yolanda García & Carlos Almeida

  4. Wageningen Food and Biobased Research, Wageningen University and Research, PO Box 17, 6700 AA, Wageningen, The Netherlands

    Carl Safi & Nicole Engelen-Smit

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  1. Lidia Montero
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Correspondence to Jose A. Mendiola.

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Montero, L., Sedghi, M., García, Y. et al. Pressurized Liquid Extraction of Pigments from Chlamydomonas sp. and Chemical Characterization by HPLC–MS/MS. J. Anal. Test. 2, 149–157 (2018). https://doi.org/10.1007/s41664-018-0062-8

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  • DOI: https://doi.org/10.1007/s41664-018-0062-8

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