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A sustainable approach in the management of Callosobruchus maculatus: essential oil of Protium heptaphyllum and its major compound d-limonene as biopesticides

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Abstract

The present study aimed to investigate the bioactivity of the essential oils of Protium heptaphyllum (Aubl.) (Sapindales: Burseraceae) and its major compound d-limonene in the control of Callosobruchus maculatus (Fabr.) (Coleoptera: Chrysomelidae: Bruchinae). The compounds in the oil were identified by gas chromatography–mass spectrometry (GC–MS). Contact toxicity, fumigation, and repellency tests were conducted. The lethal concentrations for the contact and fumigation tests were determined. In the repellency tests, the lethal concentrations previously determined in the contact test were used. The results showed that d-limonene was the major compound (40.1%) in the essential oil of P. heptaphyllum. d-limonene and P. heptaphyllum oil showed toxicity to C. maculatus in the contact and fumigation tests, and the oil showed higher toxicity (14.23 μL/20 g and 191.28 μL/L air), therefore being more toxic than d-limonene (36.42 μL/20 g and 437.34 μL/L air). The number of insects that emerged in the contact and repellency tests was reduced at all concentrations, which shows that the oil and d-limonene have an ovicidal effect. Repellent effects of P. heptaphyllum and d-limonene on C. maculatus were observed. The essential oil of P. heptaphyllum, which is more toxic than its major compound, has potential as an insecticide against C. maculatus.

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Authors and Affiliations

  1. Graduate Program in Agronomy/Tropical Agriculture, Universidade Federal do Piauí, Campus da Socopo S/N, 64.049-550, Teresina, PI, Brasil

    Rosalba Maria Borges de Andrade Rodrigues, Rodrigo de Carvalho Brito & Emanoela Maria de Jesus Sousa

  2. Department of Biology, Center for Natural Sciences, Universidade Federal do Piauí, Campus Ministro Petrônio Portela S/N, 64, Teresina, PI, 64.049-550, Brasil

    Lucia da Silva Fontes

  3. Department of Chemistry, Center for Natural Sciences, Universidade Federal do Piauí, Campus Ministro Petrônio Portela S/N, Teresina, PI, 64.049-550, Brasil

    Antonia Maria das Graças Lopes Citó & Iolanda Souza do Carmo

  4. Instituto Federal de Educação, Ciência Tecnologia do Mato Grosso do Sul Campus Nova Andradina, Rod. MS-473, km. 23, Nova Andradina, MS, 79750-000, Brasil

    Gutierres Nelson Silva

  5. Instituto Federal de Educação, Ciência e Tecnologia do Maranhão Campus Codó, Povoado Poraque, Zona Rural, Codó, MA, 65400-000, Brasil

    Douglas Rafael e Silva Barbosa

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  1. Rosalba Maria Borges de Andrade Rodrigues
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  2. Lucia da Silva Fontes
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  3. Rodrigo de Carvalho Brito
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  4. Douglas Rafael e Silva Barbosa
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RMBAR; Fontes, LS participated in all stages of manuscript production; AMGLC and ISC contributed to the chromatographic analysis; RCB and EMJS participated in the execution of the experiments; DRSB and GNS contributed with statistical analysis and helped to write the manuscript.

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Correspondence to Douglas Rafael e Silva Barbosa.

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de Andrade Rodrigues, R.M.B., da Silva Fontes, L., de Carvalho Brito, R. et al. A sustainable approach in the management of Callosobruchus maculatus: essential oil of Protium heptaphyllum and its major compound d-limonene as biopesticides. J Plant Dis Prot 129, 831–841 (2022). https://doi.org/10.1007/s41348-022-00617-4

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