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Effects of Myrothecium verrucaria ZW-2 fermentation filtrates on various plant-parasitic nematodes

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Abstract

This study shows for the first time the effect of the fermentation filtrate of Myrothecium verrucaria, strain ZW-2, on four different species of plant-parasitic nematodes. Our results suggested that the fermentation filtrate of M. verrucaria inhibited hatching of Meloidogyne incognita and Heterodera glycines eggs. The lowest egg hatching rates of H. glycines and M. incognita were 6.3% and 2.0%, respectively, after 15 days of incubation with a fermentation filtrate of M. verrucaria. A 2-week fermentation filtrate had lethal effects on of M. incognita (second stage juveniles), H. glycines and Bursaphelenchus xylophilus. After 72 h of incubation, the following mortality rates were observed: M. incognita J2s, 100%; Hirschmanniella spp., 8.4%; H. glycines J2s, 82.4%; and B. xylophilus, 96.1%. A pot test showed that the mean suppressive rates of H. glycines after one- and two-week fermentation treatments were 78.8% and 91.2%, respectively. There were 0.8 nematodes per tomato root system at seven days after inoculation (DAI) and 4.4 galls and 933.3 eggs per root system at 30 DAI, which were significantly lower than those of the tap water controls (8.0 nematodes, 31.4 galls and 14,622.5 eggs per root system). Our studies confirmed that the fermentation filtrate of strain ZW-2 has nematicidal effects against three plant-parasitic nematodes.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (31660511, 31460464) and the Special Fund for Agro-Scientific Research in the Public Interest (201503114). We would like to thank American Journal Experts (https://www.aje.com) for editing and reviewing this manuscript for English language.

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  1. Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety, Agricultural College of Guangxi University, Nanning, 530004, China

    Hai Yan Wu, Lu Yuan Zhang & Xun Bo Zhou

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  1. Hai Yan Wu
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  2. Lu Yuan Zhang
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Correspondence to Xun Bo Zhou.

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Wu, H.Y., Zhang, L.Y. & Zhou, X.B. Effects of Myrothecium verrucaria ZW-2 fermentation filtrates on various plant-parasitic nematodes. J Plant Dis Prot 127, 545–552 (2020). https://doi.org/10.1007/s41348-020-00336-8

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