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Identification and characterization of two phytoplasma subgroups (16SrXI-D and 16SrXIV-A) associated with lychee (Litchi chinensis Sonn) in India

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Abstract

Leaf rolling, necrosis and apical bud proliferation symptoms were recorded on lychee plants at Bhatpar Rani (Deoria district) and Gorakhpur (Gorakhpur district) of Eastern Uttar Pradesh, India, in 2013–2014. Symptomatic lychee leaf samples were analyzed for phytoplasma association by nested PCR assays which yielded ~1.2 kb amplicons with R16F2n/R16R2 primer pair from the symptomatic samples of both the places. Pairwise sequence comparison and phylogenetic analysis of 16S rRNA gene sequences of lychee phytoplasma strains from Deoria and Gorakhpur confirmed the association of two groups (16SrXI and 16SrXIV) of phytoplasmas. Virtual analysis of 16S rDNA sequences of both the lychee phytoplasma strains classified them as 16SrXI-D (Deoria) and 16SrXIV-A (Gorakhpur) subgroups, which are new reports to the world.

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Acknowledgements

Authors are thankful to Dr. R. Manimekalai, Sugarcane Breeding Institute, Coimbatore, for her help in preparing the virtual RFLP gel image of the phytoplasma strains in the study and ICAR Extramural Research, New Delhi for financial assistance.

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Authors and Affiliations

  1. Division of Plant Pathology, Indian Agriculture Research Institute, Pusa Campus, New Delhi, 110012, India

    Govind P. Rao &  Madhupriya

  2. Central Lab, U P Council of Sugarcane Research, Shajhanpur, UP, 242001, India

    Ajay K. Tiwari

  3. Department of Botany, DDU Gorakhpur University, Gorakhpur, UP, 273008, India

    Durgesh Dubey

Authors
  1. Govind P. Rao
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  2. Ajay K. Tiwari
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  3. Madhupriya
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  4. Durgesh Dubey
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Correspondence to Ajay K. Tiwari.

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Rao, G.P., Tiwari, A.K., Madhupriya et al. Identification and characterization of two phytoplasma subgroups (16SrXI-D and 16SrXIV-A) associated with lychee (Litchi chinensis Sonn) in India. J Plant Dis Prot 124, 235–239 (2017). https://doi.org/10.1007/s41348-017-0084-x

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  • DOI: https://doi.org/10.1007/s41348-017-0084-x

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