Abstract
Insomnia is associated with anxiety and memory deficits. α-Asarone, an active principle of Acorus species, which has anxiolytic and memory-restoring properties, was recently identified as a potential hypnotic with the least side effects. The current study was conducted in rats to find out whether the hypnotic dose of α-asarone can produce anxiolytic and memory restoring effects. α-Asarone (10 mg/kg, i.p.) and its vehicle were administered to rats, deprived of sleep by gentle handling for 5 h for 5 days. Spatial memory was assessed in the radial arm maze. Anxiety was tested using elevated plus maze and open field test. In addition to using the vehicle of the drug as a control, midazolam (2 mg/kg, i.p.), a known anxiolytic, was also used as a positive control. Increased anxiety levels produced after sleep deprivation were reduced after α-asarone administration. The anxiolytic effect was comparable to that produced by midazolam. Increase in the reference and working memory errors, resulting from sleep deprivation, was reduced after α-asarone injection. It also produced an improvement in the percentage of correct choices. Decreased lipid peroxidation and increased activities of catalase and glutathione reductase facilitated the improvement in cognitive functioning. α-Asarone, is not only a good therapeutic agent for insomnia and associated anxiety, it can also reduce insomnia-associated memory deficits. This is the first report on the anxiolytic and cognition enhancing property of α-asarone in sleep-deprived rats.
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Abbreviations
- SD:
-
Sleep deprivation
- EPM:
-
Elevated plus maze
- OFT:
-
Open field test
- RAM:
-
Radial arm maze
- MDA:
-
Malondialdehyde
- GSH-R:
-
Glutathione reductase
- CAT:
-
Catalase
- SOD:
-
Superoxide dismutase
- GSH-Px:
-
Glutathione peroxide
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Acknowledgements
The work was supported by the research grant from the Council of Scientific and Industrial Research, New Delhi, India (CSIR Sanction No: 37(1543)/12-EMR II). AR was supported by CSIR Junior Research Fellowship. We acknowledge Dr. Lakshmi R for her assistance in the RAM test.
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None of the authors has any financial interest or conflicts of interest related to this work.
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All the procedures employed in this study were approved by the Institutional Animal Ethics Committee of the Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, Kerala.
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Wistar rats were obtained from the Division of Laboratory Animal Sciences, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, Kerala.
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Radhakrishnan, A., Jayakumari, N., Kumar, V.M. et al. α-Asarone in management of sleep deprivation induced memory deficits and anxiety in rat model. Sleep Biol. Rhythms 17, 37–47 (2019). https://doi.org/10.1007/s41105-018-0181-7
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DOI: https://doi.org/10.1007/s41105-018-0181-7