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Statistical Optimization of Cellulase Production by Thermomyces dupontii

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Abstract

In the present investigation, significant physical parameters such as incubation time, temperature and pH were optimized for cellulase production by wild and mutant strains of thermophilic Thermomyces dupontii. For a comparative analysis, three independent variables which were selected from OVAT were subjected to RSM. As per response surface methodology (RSM) model, central composite design was used in order to analyze the interactive effects of significant physical factors on cellulase productivity. The second polynomial regression model with a determination coefficient R2 value 0.9503 and 0.9711 was found to be adequate and significant. 3D response surface plots and contours showed interaction among three variables. In contrast to wild T. dupontii, mutant strain showed 1.7-fold increase in FPase activity (43 U/ml/min) at 55 °C and pH 5.5 in 72 h. The result indicates thermophilic mutant T. dupontii has a potential for industrial use, because enzyme obtained from thermophile is more appropriate for industrial process where high temperature is involved.

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Authors and Affiliations

  1. Department of Biotechnology, Lahore Collage for Women University, Lahore, Pakistan

    Kinza Nisar, Roheena Abdullah, Afshan Kaleem & Mehwish Iqtedar

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  1. Kinza Nisar
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  2. Roheena Abdullah
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  3. Afshan Kaleem
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  4. Mehwish Iqtedar
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Correspondence to Roheena Abdullah.

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Nisar, K., Abdullah, R., Kaleem, A. et al. Statistical Optimization of Cellulase Production by Thermomyces dupontii. Iran J Sci Technol Trans Sci 44, 1269–1277 (2020). https://doi.org/10.1007/s40995-020-00932-1

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  • DOI: https://doi.org/10.1007/s40995-020-00932-1

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