Abstract
Cassava mosaic disease (CMD), a serious threat to cassava production, is caused by two begomovirus species in Asia, Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus (SLCMV). Cassava mosaic disease has recently spread into Southeast Asia and it is urgent to establish an efficient system to monitor its spread. Cassava fields were surveyed for CMD on the web using a smartphone-based farming support application, AGRIBUDDY, to obtain photos of CMD and their time, location, and contact information posted to the AGRIBUDDY cloud by farmers. To diagnose a pathogen, we designed novel PCR primers for CMD identification. By combining these two techniques, we detected SLCMV occurrence in Kampong Thom, Cambodia.
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References
Barbedo JGA, Koenigkan LV, Santos TT (2016) Identifying multiple plant diseases using digital image processing. Biosystems Engineering 147:104–116
Camargo A, Smith JS (2009) An image-processing based algorithm to automatically identify plant disease visual symptoms. Biosystems Engineering 102:9–21
Czosnek H, Hariton-Shalev A, Sobol I, Gorovits R, Ghanim M (2017) The incredible journey of begomoviruses in their whitefly vector. Viruses 9:273
Kushwaha N, Singh AK, Chattopadhyay B, Chakraborty S (2010) Recent advances in geminivirus detection and future perspectives. The Journal of Plant Protection Sciences 2:1–18
Mahlein AK (2016) Plant disease detection by imaging sensors: parallels and specific demands for precision agriculture and plant phenotyping. Plant Disease 100:241–251
Polder G, van der Heijden GWAM, van Doorn J, Baltissen TAHMC (2014) Automatic detection of Tulip breaking virus (TBV) in tulip fields using machine vision. Biosystems Engineering 117:35–42
Raji SN, Subhash N, Ravi V, Saravanan R, Mohanan CN, Kumar TM, Nita S (2016) Detection and classification of mosaic virus disease in cassava plants by proximal sensing of photochemical reflectance index. Journal of the Indian Society of Remote Sensing 44:875–883
Saunders K, Saim N, Mali VR, Malathi VG, Briddon R, Markham PG, Stanley J (2002) Characterisation of Sri Lankan cassava mosaic virus and Indian cassava mosaic virus: evidence for acquisition of a DNA-B component by a monopartite begomovirus. Virology 293:63–74
Sseruwagi P, Sserubombwe WS, Legg JP, Ndunguru J, Thresh JM (2004) Methods of surveying the incidence and severity of cassava mosaic disease and whitefly vector populations on cassava in Africa: a review. Virus Research 100:129–142
Wang HL, Cui XY, Wang XW, Liu SS, Zhang ZH, Zhou XP (2016) First report of Sri Lankan cassava mosaic virus infecting cassava in Cambodia. Plant Disease 100:1029
Zerbini FM, Briddon RW, Idris A, Martin DP, Moriones E, Navas-Castillo J, Rivera-Bustamante R, Roumagnac P, Varsani A, ICTV Report Consortium (2017) ICTV virus taxonomy profile: Geminiviridae. Journal of General Virology 98:131–133
Acknowledgements
This research was supported by the Science and Technology Research Partnership for Sustainable Development (SATREPS), Japan Science and Technology Agency (JST) and Japan International Cooperation Agency (JICA).
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Uke, A., Khin, S., Kitaura, K. et al. Combination of an image-posting system and molecular diagnosis for detecting Sri Lankan cassava mosaic virus. Trop. plant pathol. 44, 238–243 (2019). https://doi.org/10.1007/s40858-019-00274-3
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DOI: https://doi.org/10.1007/s40858-019-00274-3