Abstract
Solanum lycopersicum is among the most important crops in Brazil. This crop is affected by a large range of fungal diseases that are recognized as major limitations for tomato production. Recently, plants grown in a greenhouse in Viçosa, Minas Gerais, Brazil, were found to bear severe blight symptoms. A pycnidial coelomycete was repeatedly found in association with necrotic tissues. The fungus had its morphology recognized as equivalent to that of Phoma and related genera. A phylogenetic analysis based on nrDNA (ITS) and partial β-tubulin (TUB) sequences led to the conclusion that the fungus involved was Phoma destructiva. Pathogenicity tests showed that, after 5 days, blight symptoms developed on leaves, flowers and stems of plants belonging to thirteen different tomato varieties tested. This fungal species is mostly known for causing post-harvest tomato rot, which is only regarded as a secondary disease in Brazil. This is in disagreement with the observations made in this work. Here, the disease symptoms caused by the fungus were very severe, fully justifying the scientific name of the pathogen. Under favorable environmental conditions, aggressive strains of P. destructiva, such as the one isolated in this study, may become significant threats to tomato plantations in Brazil.
References
Aveskamp MM, de Gruyter J, Woudenberg JHC, Verkley GJM, Crous PW (2010) Highlights of the Didymellaceae: a polyphasic approach to characterize Phoma and related pleosporalean genera. Studies in Mycology 65:1–60
Batista MF, Alves MLB (1981) Doenças do tomateiro na região de Manaus. Fitopatologia Brasileira 6:573–574
Chen Q, Jiang JR, Zhang GZ, Cai L, Crous PW (2015) Resolving the Phoma enigma. Studies in Mycology 82:137–217
Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32:1792–1797
Farr DF, Rossman AY (2016) Fungal databases, systematic mycology and microbiology laboratory, ARS, USDA. Available at: http://nt.ars-grin.gov/fungaldatabases/. Accessed 10 June 2016
Glass NL, Donaldson GC (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous Ascomycetes. Applied and Environmental Microbiology 61:1323–1330
Gruyter J, Boerema GH, van der Aa HA (2002) Contributions towards a monograph of Phoma (Coelomycetes)—VI-2. Section Phyllostictoides: outline of its taxa. Persoonia 18:1–53
Gruyter J, Woudenberg JHC, Aveskamp MM, Verkley GJM, Groenewald JZ, Crous PW (2012) Redisposition of Phoma-like anamorphs in Pleosporales. Studies in Mycology 75:1–36
Hepperle D (2011) DNA Dragon 1.4.1 - DNA Sequence Contig Assembler Software. Available at: www.dna-dragon.com. Accessed 16 June 2016
Inouie-Nagata AK, Lopes CA, Reis A, Pereira RB, Quezado-Duval JB, Lima MF (2016) Doenças do Tomateiro. In: Amorim L, Rezende JAM, Bergamin Filho A, Camargo LEA (eds) Manual de Fitopatologia. Vol. 2. Doenças das Plantas Cultivadas. Ceres, São Paulo, pp 697–732
Jones JB, Zitter TA, Momol TM, Miller SA (2014) Compendium of tomato diseases and pests, 2nd edn. APS Press, St. Paul
Kimati H, Amorim L, Bergamin Filho A, Camargo LEA, Rezende JAM (2005) Manual de Fitopatologia: Doenças das Plantas Cultivadas, vol 2, 2nd edn. Editora Agronômica Ceres Ltda, São Paulo
Lopes CA, de Ávila AC (2005) Doenças do tomateiro. Embrapa Hortaliças, Brasília
Miller MA, Pfeiffer W, Schwartz T (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the Gateway Computing Environments Workshop (GCE). New Orleans. pp. 1–8
O’Donnell K, Cigelnik E (1997) Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are non-orthologous. Molecular Phylogenetics and Evolution 7:103–116
Posada D, Buckley TR (2004) Model selection and model averaging in phylogenetics: advantages of Akaike information criterion and Bayesian approaches over likelihood ratio tests. Systematic Biology 53:793–808
Pinho DB, Firmino AL, Pereira OL, Ferreira Junior WG (2012) An efficient protocol for DNA extraction from Meliolales and the description of Meliola centellae sp. nov. Mycotaxon 122:333–345
Rashid TS, Sijam K, Nasehi A, Kadir J (2016) Occurence of Phoma blight caused by Phoma destructiva on tomato (Solanum lycopersicum) in Malaysia. Plant Disease 100:1241–1242
Robl D, Thimoteo SS, de Souza GCCF, Beux MR, Dalzoto PR, Pinheiro RL, Pimentel IC (2014) Occurrence of Candida orthopsilosis in Brazilian tomato fruits (Lycopersicum esculentum Mill.) Brazilian Journal of Microbiology 45:105–109
Ronquist F, Heulsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574
Sung GH, Sung JM, Hywel Jones NL, Spatafora JW (2007) A multi-gene phylogeny of Clavicipitaceae (Ascomycota, Fungi): identification of localized incongruence using a combinational bootstrap approach. Molecular Phylogenetics and Evolution 44:1204–1223
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28:2731–2739
Vale FXR, Jesus Junior WC, Rodrigues FA, Costa H, Souza CA (2007) Manejo de doenças fúngicas em tomateiro. In: da Silva JHL, Vale FXR. (Org.) Tomate: tecnologia de produção. Suprema, Visconde do Rio Branco, pp 159–197
White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315–322
Acknowledgements
The authors wish to thank Fundação de Amparo à Pesquisa do Estado de Minas Gerais - FAPEMIG, Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES for financial support of the work.
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Section Editor: Trazilbo J. Paula Jr.
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Colmán, A.A., Alves, J.L., da Silva, M. et al. Phoma destructiva causing blight of tomato plants: a new fungal threat for tomato plantations in Brazil?. Trop. plant pathol. 43, 257–262 (2018). https://doi.org/10.1007/s40858-017-0200-2
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DOI: https://doi.org/10.1007/s40858-017-0200-2