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Bacterial resistance in AtNPR1 transgenic sweet orange is mediated by priming and involves EDS1 and PR2

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Abstract

In sweet orange, varietal resistance is crucial to avoid citrus canker, a disease caused by Xanthomonas citri subsp. citri (Xcc) that is a significant problem for some of the world’s leading producers. In the present study, transgenic sweet orange plants (Hamlin) overexpressing the A. thaliana Npr1 (non-expressor of pathogenesis-related [PR] genes) gene were produced and assessed for Xcc resistance and for the levels of expression of disease resistance genes. The leaves of these transgenic plants exhibited fewer lesions and reduced bacterial concentrations compared to non-transformed control. Both in vitro and screenhouse experiments on the transgenic plants yielded similar results, which makes in vitro screening an interesting prospect for the initial identification of citrus canker resistance. The stronger expression of EDS1 and PR2 after pathogen inoculation correlated with a higher tolerance response suggesting a possible priming effect. The use of AtNPR1 is an alternative against citrus canker since it led to higher tolerance in a pathogen-dependent response

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Acknowledgments

The authors wish to thank Simone C. Picchi, Janaynna M. Barbosa-Mendes and Luis F. C. da Silva (Centro de Citricultura Sylvio Moreira, IAC) for assistance with Xcc inoculation and screenhouse plant propagation. We also thank Dr. Adrián A. Vojnov (Instituto de Ciencia y Tecnología Dr. Cesar Milstein, Fundación Pablo Cassará, CONICET, Argentina) for providing the Xcc_GFP. This work was supported by FAPESP (grants 09/00058-3, 01/02616-1, INCT-Citros 08/57909-2) and CNPq (grants 471419/2012-5, INCT-Citros 573848/08-4). M.A.M. and M.A.T. are recipients of research fellowships from CNPq.

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  1. Centro de Citricultura Sylvio Moreira, Instituto Agronômico, Rodovia Anhanguera, km 158, 13490-970, PO Box 04, Cordeirópolis, SP, Brazil

    Raquel L. Boscariol-Camargo, Marco A. Takita & Marcos A. Machado

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  1. Raquel L. Boscariol-Camargo
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Correspondence to Raquel L. Boscariol-Camargo.

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Boscariol-Camargo, R.L., Takita, M.A. & Machado, M.A. Bacterial resistance in AtNPR1 transgenic sweet orange is mediated by priming and involves EDS1 and PR2 . Trop. plant pathol. 41, 341–349 (2016). https://doi.org/10.1007/s40858-016-0108-2

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