Abstract
The CRISPR-Cas9 system, naturally a defense mechanism in prokaryotes, has been repurposed as an RNA-guided DNA targeting platform. It has been widely used for genome editing and transcriptome modulation, and has shown great promise in correcting mutations in human genetic diseases. Off-target effects are a critical issue for all of these applications. Here we review the current status on the target specificity of the CRISPR-Cas9 system.
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Wu, X., Kriz, A.J. & Sharp, P.A. Target specificity of the CRISPR-Cas9 system. Quant Biol 2, 59–70 (2014). https://doi.org/10.1007/s40484-014-0030-x
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DOI: https://doi.org/10.1007/s40484-014-0030-x