Skip to main content
SpringerLink
Log in

Pleopeltis lepidopteris Langsd. & Fisch. (Polypodiaceae), an endemic fern from Brazilian “restingas”: viability of spores under different storage conditions

  • Original Article
  • Published:
Brazilian Journal of Botany Aims and scope Submit manuscript

Abstract

Spores of Pleopeltis lepidopteris (Polypodiaceae) were stored under different temperatures. Samples of spores were stored at room temperature, under refrigeration at 7 ± 1 °C (1 and 8 months), and in liquid nitrogen (1 and 8 months). After storage, spore viability and gametophyte development were analyzed. When stored at room temperature, spores of P. lepidopteris quickly lost viability after storage for 1 month and died after 8 months. When spores were stored dry at 7 °C, they showed higher percentages of germination, but there was a significant decrease in viabilities after 8 months of storage. In contrast, when stored in liquid nitrogen, spores survived and presented a high percentage of germination. Moreover, gametophyte development was accelerated in the cryopreserved spores relative to those grown from freshly collected spores. Thus, for this endemic species of the Brazilian sandbank, cryogenic storage of spores is an effective method for “ex situ” conservation in gene banks.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figs. 1–8

Similar content being viewed by others

References

  • Agrawal DC, Pawar SS, Mascarenhas AF (1993) Cryopreservation of spores of Cyathea spinulosa (Wall. ex Hook.) an endangered fern. J Plant Physiol 142:124–126

    Article  Google Scholar 

  • Alvares CA, Stape JL, Sentelhas PC, Gonçalves JLM, Sparovek G (2013) Köppen’s climate classification map for Brazil. Meteorol Z 22:711–728

    Article  Google Scholar 

  • Aragon CF, Pangua E (2004) Spore viability under different storage conditions in four Asplenium rupicolous L. taxa. Am Fern J 94:28–38

    Article  Google Scholar 

  • Ashcroft CJ, Sheffield E (2000) The effects os spore density on germination and development in Pteridium, monitored using a novel culture technique. Am Fern J 90:91–99

    Article  Google Scholar 

  • Ballesteros D (2010) Conservation of fern spore. In: Fernández H, Kumar A, Ashwani R (eds) Working with ferns. Springer, New York, pp 165–172

    Google Scholar 

  • Ballesteros D, Estrelles E, Ibars AM (2006) Responses of pteridophyte spores to ultrafreezing temperatures for long-term conservation in germplasm banks. Fern Gaz 17:293–302

    Google Scholar 

  • Ballesteros D, Estrelles E, Walters C, Ibars AM (2011) Effect of storage temperature on green spore longevity for the fern Equisetum ramosissimum and Osmunda regalis. Cryo-Lett 32:89–98

    CAS  Google Scholar 

  • Ballesteros D, Estrelles E, Walters C, Ibars AM (2012) Effects of temperature and desiccation on ex situ conservation of nongreen fern spores. Am J Bot 99:721–729

    Article  PubMed  Google Scholar 

  • Begnini RM, Randi AM (2010) Viabilidade de esporos de Dicksonia sellowiana Hook. (Cyatheales, Dicksoniaceae) e Rumohra adiantiformis (Forst.) Ching (Polypodiales, Dryopteridaceae) armazenados sob refrigeração. Insula 38:15–27

    Article  Google Scholar 

  • Benson EE, Lynch PT, Stacey GN (1998) Advances in plant cryopreservation technology: current applications in crop plant biotechnology. Agric Biot News Inf 10:133–141

    Google Scholar 

  • Beri A, Bir SS (1993) Germination of stored spores of Pteris vittata L. Am Fern J 83:73–78

    Article  Google Scholar 

  • Brum FMR, Randi AM (2006) Germination of spores and growth of gametophytes and sporophytes of Rumohra adiantiformis (Forst.) Ching (Dryopteridaceae) after spore cryogenic storage. Braz J Bot 29:489–495

    Article  Google Scholar 

  • Camloh M (1993) In vitro culture of Platycerium bifurcatum gametophytes. Am Fern J 83:79–85

    Article  Google Scholar 

  • Camloh M (1999) Spore age and sterilization affects germination and early gametophyte development of Platycerium bifurcatum. Am Fern J 89:124–132

    Article  Google Scholar 

  • CONAMA (1999) Resolução 261, que trata dos estágios sucessionais de vegetação de restinga para o estado de Santa Catarina, p 12

  • Dancey C, Reidy J (2006) Estatística sem matemática para psicologia: usando SPSS para windows. Artmed, Porto Alegre

    Google Scholar 

  • Dyer AF (1979) The culture of fern gametophytes for experimental investigation. In: Dyer AF (ed) The experimental biology of ferns. Academic Press, Cambridge, pp 253–305

    Google Scholar 

  • Filipin EP, Schmidt EC, Barufi JB, Bouzon ZL, Randi AM (2016) The gametophyte of Pleopeltis lepidopteris (Langsd. and Fisch) de la Sota (Polypodiaceae), a fern from “restinga”, after spore cryopreservation: morphological, ultrastructural and physiological analyses. Int J Plant Sci 177:294–303

    Article  Google Scholar 

  • Labiak PH, Hirai RY (2014) Polypodiaceae in Lista de Espécies da Flora do Brasil. Jardim Botânico do Rio de Janeiro. Disponível em: http://reflora.jbrj.gov.br/jabot/floradobrasil/FB111504>. Acesso em 29 Mai 2014

  • Lacerda LD, Araújo DSD, Maciel NC (1993) Dry coastal ecosystems of the tropical Brazilian coast. In: Van der Mararel E (ed) Dry coastal ecosystems of the world. Elsevier, Amsterdam, pp 477–493

    Google Scholar 

  • Li Y, Shi L (2014) Effect of desiccation level and storage temperature on green spore viability of Osmunda japonica. Cryobiology 68:446–450

    Article  PubMed  Google Scholar 

  • Li Y, Zhang YL, Jiang CD, Wang T, Wang Q, Shi L (2010) Effect of storage temperature on spore viability and early gametophyte development of three vulnerable species of Alsophila (Cyatheaceae). Aust J Bot 58:89–96

    Article  Google Scholar 

  • Lloyd RM, Klekowski EJ Jr (1970) Spore germination and viability in pteridophyta: evolutionary significance of chlorophyllous spores. Biotropica 2:129–137

  • Mikula A, Jata K, Rybczy´nski JJ (2009) Cryopreservation strategies for Cyathea australis (R.Br.) Domin. Cryo-Lett 30:429–439

    CAS  Google Scholar 

  • Pence VC (2000) Survival of chlorophyllous and nonchlorophyllous fern spores through exposure to liquid nitrogen. Am Fern J 90:119–126

    Article  Google Scholar 

  • Pence VC (2004) Ex situ conservation methods for bryophytes and pteridophytes. In: Guerrant EO, Havens K, Maunder M (eds) Ex situ plant conservation: supporting species survival in the wild. Press, Island, pp 206–227

    Google Scholar 

  • Pence VC (2008a) Ex situ conservation of ferns and lycophytes: approaches and techniques. In: Ranker TA, Haufler CH (eds) Biology and evolution of ferns and lycophytes. Cambridge Univ Press, Cambridge, pp 284–300

    Chapter  Google Scholar 

  • Pence VC (2008b) Cryopreservation of bryophytes and ferns. In: Reed BM (ed) Plant cryopreservation: A practical guide. Springer, Berlin, pp 117–140

    Chapter  Google Scholar 

  • Quintanilla LG, Amigo J, Pangua E, Pajarón S (2002) Effect of storage method on spore viability in five threatened fern species. Ann Bot 90:461–467

    Article  PubMed  PubMed Central  Google Scholar 

  • Randi AM (1996) Photosensivity, viability and storage reserves in spores of Acrostichum danaeifolium Langsd. and fisch. (Pteridaceae). Braz J Bot 19:105–108

    Google Scholar 

  • Rocha CFD, Nunes-Freitas AF, Rocha-Pessôa TC, Cogliatti-Carvalho L (2004) Habitat disturbance in Brazilian Coastal sand dune vegetation and present richness and diversity of bromeliad species. Vidalia 2:50–56

    Google Scholar 

  • Rogge GD, Viana AM, Randi AM (2000) Cryopreservation of spores of Dicksonia sellowiana. An endangered tree fern indigenous to South and Central America. Cryo-Lett 21:223–230

    CAS  Google Scholar 

  • Simabukuro EA, Dyer AF, Felippe GM (1998) The effect of sterilization and storage conditions on the viability of the spores of Cyathea delgadii. Am Fern J 88:72–80

    Article  Google Scholar 

  • Smith AR, Tejero-Díez JD (2014) Pleopeltis (Polypodiaceae) una redefinición genérica y novedades nomenclaturales. Bot Sci 92:43–58

    Article  Google Scholar 

  • Smith AR, Pryer KM, Schuettpelz E, Korall P, Schneider H, Wolf PG (2006) A classification for extant ferns. Taxon 55:705–731

    Article  Google Scholar 

  • Tryon RM, Tryon AF (1982) Ferns and allied plants with special reference to tropical America. Springer, New York

    Book  Google Scholar 

  • Viviani D, Randi AM (2008) Effects of pH, temperature and light on spore germination and growth analysis of young sporophytes of Polypodium lepidopteris (Pteridophyta, Polypodiaceae). Rodriguésia 59:751–760

    Google Scholar 

  • Windham MD, Wolf PG, Ranker TA (1986) Factors affecting prolonged spore viability in herbaceous collections of three species of Pellaea. Am Fern J 76:141–148

    Article  Google Scholar 

Download references

Acknowledgments

The authors thank the graduate program in Fungi, Algae and Plants (FAP) of the Departament of Botany of the Federal University of Santa Catarian, Brazil. This study was supported in part by the Coordination of Improvement of Higher Education Personal (CAPES). The authors thank CAPES for providing a scholarship to Elisa Poltronieri Filipin.

Author information

Authors and Affiliations

  1. Post-graduation Program in Biology of Fungos Algae and Plant, Department of Botany (UFSC), Federal University of Santa Catarina, Florianópolis, SC, Brazil

    Elisa Poltronieri Filipin

  2. Post-graduation Program in Biology Cell Biology Embryology and Genetics, Department of Cell Biology, Embryology and Genetics (UFSC), Federal University of Santa Catarina, Florianópolis, SC, Brazil

    Carmen Simioni & Éder Carlos Schmidt

  3. Department of Botany (UFSC), Federal University of Santa Catarina, Florianópolis, SC, Brazil

    Áurea Maria Randi

Authors
  1. Elisa Poltronieri Filipin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Carmen Simioni
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Éder Carlos Schmidt
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Áurea Maria Randi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Áurea Maria Randi.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Filipin, E.P., Simioni, C., Schmidt, É.C. et al. Pleopeltis lepidopteris Langsd. & Fisch. (Polypodiaceae), an endemic fern from Brazilian “restingas”: viability of spores under different storage conditions. Braz. J. Bot 40, 59–65 (2017). https://doi.org/10.1007/s40415-016-0332-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40415-016-0332-5

Keywords

Search

Navigation